Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital

• Autor(a) principal: Corrêa,Laís Lisboa
• Data de Publicação: 2012
• Outros Autores: Botelho,Larissa Alvarenga Batista, Barbosa,Lívia Carvalho, Mattos,Claudio Simões, Carballido,Jupira Miron, Castro,Carmem Lúcia Teixeira de, Mondino,Pedro Juan Jose, Paula,Geraldo Renato de, Mondino,Silvia Susana Bona de, Mendonça-Souza,Claudia Rezende Vieira de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Infectious Diseases
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600004
• topic: Acinetobacter spp; OXA-23; Carbapenem resistance

INTRODUCTION: Acinetobacter spp. have emerged as notorious pathogens involved in healthcareassociated infections. Carbapenems are important antimicrobial agents for treating infections due to multidrug resistant Acinetobacter spp. Different mechanisms may confer resistance to these drugs in the genus, particularly production of class D carbapenemases. OXA-23-like family has been pointed out as one of the predominant carbapenamases among Acinetobacter. The present work aimed to investigate the occurrence of OXA-23-like carbapenemases among Acinetobacter isolates recovered from patients of a university hospital in Niterói, RJ, Brazil. METHODS: Antimicrobial susceptibility profiles were determined by disk-diffusion. Imipenem resistant isolates were submitted to Modified Hodge Test in order to screen for carbapenemase production, and later to polymerase chain reaction (PCR) to investigate the presence of blaOXA-23. RESULTS: Imipenem and meropenem resistance rates were 71.4% and 69.7%, respectively. The Modified Hodge Test revealed carbapenemase production among 76 (89.4%) of the 85 imipenem resistant isolates analyzed; according to PCR results, 81 isolates (95.4%) carried the blaOXA-23 gene. CONCLUSIONS: OXA-23-like enzymes may be an important mechanism of carbapenem resistance among isolates present in the hospital studied.