Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients
Main Author: | |
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Publication Date: | 2016 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Brazilian Journal of Infectious Diseases |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500468 |
Summary: | Abstract Objectives To compare cultured microorganisms identified on endotracheal tubes biofilms through sonication technique with traditional tracheal aspirate collected at extubation of pediatric intensive care unit patients. Methods Demographic and epidemiological data were analyzed to identify factors possibly related with the microbiological profile of the two collection methods. Associations between categorical and continuous variables were analyzed using the chi-square or Fisher's exact test, or Student's t test. p-Value <0.05 were considered significant. Results Thirty endotracheal tubes and tracheal aspirates samples from 27 subjects were analyzed. Only one patient presented the clinical diagnosis of ventilator-associated pneumonia. Overall, 50% of bacteria were Gram-negative bacilli, followed by Gram-positive bacteria in 37%, and fungi in 10%. No statistically significant difference on the distribution of Gram-positive or Gram-negative bacteria (p = 0.996), and fungi (p = 0.985) were observed between the collection methods. Pseudomonas spp. was the most frequent microorganism identified (23.8%), followed by Streptococcus spp. (18.5%), Acinetobacter spp. (15.9%), coagulase-negative staphylococci (11.2%), and Klebsiella spp. (8.6%). Concordant results between methods amounted to 83.3%. Pseudomonas aeruginosa and Acinetobacter baumannii showed carbapenem resistance in 50% and 43.7% of the isolates, respectively. In general, cultures after endotracheal tubes sonication (non-centrifuged sonication fluid and centrifuged sonication fluid) yielded bacteria with higher rates of antimicrobial resistance compared to tracheal aspirates cultures. Additionally, in 12 subjects (40%), we observed discrepancies regarding microbiologic profiles of cultures performed using the collection methods. Conclusions Our study demonstrated that sonication technique can be applied to ET biofilms to identify microorganisms attached to their surface with a great variety of species identified. However, we did not find significant differences in comparison with the traditional tracheal aspirate culture approach. |
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Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patientsSonicationEndotracheal tubeBiofilmTracheal aspirateAbstract Objectives To compare cultured microorganisms identified on endotracheal tubes biofilms through sonication technique with traditional tracheal aspirate collected at extubation of pediatric intensive care unit patients. Methods Demographic and epidemiological data were analyzed to identify factors possibly related with the microbiological profile of the two collection methods. Associations between categorical and continuous variables were analyzed using the chi-square or Fisher's exact test, or Student's t test. p-Value <0.05 were considered significant. Results Thirty endotracheal tubes and tracheal aspirates samples from 27 subjects were analyzed. Only one patient presented the clinical diagnosis of ventilator-associated pneumonia. Overall, 50% of bacteria were Gram-negative bacilli, followed by Gram-positive bacteria in 37%, and fungi in 10%. No statistically significant difference on the distribution of Gram-positive or Gram-negative bacteria (p = 0.996), and fungi (p = 0.985) were observed between the collection methods. Pseudomonas spp. was the most frequent microorganism identified (23.8%), followed by Streptococcus spp. (18.5%), Acinetobacter spp. (15.9%), coagulase-negative staphylococci (11.2%), and Klebsiella spp. (8.6%). Concordant results between methods amounted to 83.3%. Pseudomonas aeruginosa and Acinetobacter baumannii showed carbapenem resistance in 50% and 43.7% of the isolates, respectively. In general, cultures after endotracheal tubes sonication (non-centrifuged sonication fluid and centrifuged sonication fluid) yielded bacteria with higher rates of antimicrobial resistance compared to tracheal aspirates cultures. Additionally, in 12 subjects (40%), we observed discrepancies regarding microbiologic profiles of cultures performed using the collection methods. Conclusions Our study demonstrated that sonication technique can be applied to ET biofilms to identify microorganisms attached to their surface with a great variety of species identified. However, we did not find significant differences in comparison with the traditional tracheal aspirate culture approach.Brazilian Society of Infectious Diseases2016-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500468Brazilian Journal of Infectious Diseases v.20 n.5 2016reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2016.07.003info:eu-repo/semantics/openAccessFerreira,Thiago de OliveiraKoto,Rafael YoshioLeite,Gabriel Fialkovitz da CostaKlautau,Giselle BurlamaquiNigro,StanleySilva,Cely Barreto daSouza,Ana Paula Idalgo da FonsecaMimica,Marcelo JenneCesar,Regina GrigolliSalles,Mauro José Costaeng2016-10-26T00:00:00Zoai:scielo:S1413-86702016000500468Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2016-10-26T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients |
title |
Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients |
spellingShingle |
Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients Ferreira,Thiago de Oliveira Sonication Endotracheal tube Biofilm Tracheal aspirate |
title_short |
Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients |
title_full |
Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients |
title_fullStr |
Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients |
title_full_unstemmed |
Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients |
title_sort |
Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients |
author |
Ferreira,Thiago de Oliveira |
author_facet |
Ferreira,Thiago de Oliveira Koto,Rafael Yoshio Leite,Gabriel Fialkovitz da Costa Klautau,Giselle Burlamaqui Nigro,Stanley Silva,Cely Barreto da Souza,Ana Paula Idalgo da Fonseca Mimica,Marcelo Jenne Cesar,Regina Grigolli Salles,Mauro José Costa |
author_role |
author |
author2 |
Koto,Rafael Yoshio Leite,Gabriel Fialkovitz da Costa Klautau,Giselle Burlamaqui Nigro,Stanley Silva,Cely Barreto da Souza,Ana Paula Idalgo da Fonseca Mimica,Marcelo Jenne Cesar,Regina Grigolli Salles,Mauro José Costa |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Thiago de Oliveira Koto,Rafael Yoshio Leite,Gabriel Fialkovitz da Costa Klautau,Giselle Burlamaqui Nigro,Stanley Silva,Cely Barreto da Souza,Ana Paula Idalgo da Fonseca Mimica,Marcelo Jenne Cesar,Regina Grigolli Salles,Mauro José Costa |
dc.subject.por.fl_str_mv |
Sonication Endotracheal tube Biofilm Tracheal aspirate |
topic |
Sonication Endotracheal tube Biofilm Tracheal aspirate |
description |
Abstract Objectives To compare cultured microorganisms identified on endotracheal tubes biofilms through sonication technique with traditional tracheal aspirate collected at extubation of pediatric intensive care unit patients. Methods Demographic and epidemiological data were analyzed to identify factors possibly related with the microbiological profile of the two collection methods. Associations between categorical and continuous variables were analyzed using the chi-square or Fisher's exact test, or Student's t test. p-Value <0.05 were considered significant. Results Thirty endotracheal tubes and tracheal aspirates samples from 27 subjects were analyzed. Only one patient presented the clinical diagnosis of ventilator-associated pneumonia. Overall, 50% of bacteria were Gram-negative bacilli, followed by Gram-positive bacteria in 37%, and fungi in 10%. No statistically significant difference on the distribution of Gram-positive or Gram-negative bacteria (p = 0.996), and fungi (p = 0.985) were observed between the collection methods. Pseudomonas spp. was the most frequent microorganism identified (23.8%), followed by Streptococcus spp. (18.5%), Acinetobacter spp. (15.9%), coagulase-negative staphylococci (11.2%), and Klebsiella spp. (8.6%). Concordant results between methods amounted to 83.3%. Pseudomonas aeruginosa and Acinetobacter baumannii showed carbapenem resistance in 50% and 43.7% of the isolates, respectively. In general, cultures after endotracheal tubes sonication (non-centrifuged sonication fluid and centrifuged sonication fluid) yielded bacteria with higher rates of antimicrobial resistance compared to tracheal aspirates cultures. Additionally, in 12 subjects (40%), we observed discrepancies regarding microbiologic profiles of cultures performed using the collection methods. Conclusions Our study demonstrated that sonication technique can be applied to ET biofilms to identify microorganisms attached to their surface with a great variety of species identified. However, we did not find significant differences in comparison with the traditional tracheal aspirate culture approach. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500468 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500468 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjid.2016.07.003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.20 n.5 2016 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
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1754209243783757824 |