Enrichment methodology to increase the positivity of cultures from body fluids

• Main Author: Daur,Alessandra Valle
• Publication Date: 2006
• Other Authors: Klimak Jr.,Francisco, Cogo,Laura Lúcia, Botão,Gislene Diógenes, Monteiro,Cristina Leise Bastos, Dalla Costa,Libera Maria
• Format: Article
• Language: eng
• Source: Brazilian Journal of Infectious Diseases
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600002
• topic: Body fluids; etiological agents; enrichment

Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert® blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents.