Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom
| Autor(a) principal: | |
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| Data de Publicação: | 2016 |
| Outros Autores: | , |
| Tipo de documento: | Artigo |
| Título da fonte: | Repositório da Produção Científica e Intelectual da Unicamp |
| Texto Completo: | https://hdl.handle.net/20.500.12733/8783 |
Resumo: | Agradecimentos: The authors express their gratitude to CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnologico), FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo) for financial support |
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Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venomVenenos de serpentesSnake venomsPhospholipase A2MyotoxinCrotalus oreganus lutosusBiological activitiesArtigo originalAgradecimentos: The authors express their gratitude to CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnologico), FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo) for financial supportAbstract: Commonly, phospholipases A2 (PLA2s) play key roles in the pathogenesis of the local tissue damage characteristic of crotaline and viperine snake envenomations. Crotalus oreganus lutosus snake venom has not been extensively studied; therefore, the characterization of its components represents a valuable biotechnological tool for studying pathophysiological processes of envenoming and for gaining a deeper understanding of its biological effects. In this study, for the first time, a basic PLA2 myotoxin, ColTx-I, was purified from C. o. lutosus through two chromatographic steps. ColTx-I is monomeric with calculated molecular mass weight (Mw) of 14,145 Da and a primary structure closely related to basic PLA2s from viperid venoms. The pure enzyme has a specific activity of 15.87 ± 0.65 nmol/min/mg at optimal conditions (pH 8.0 and 37 °C). ColTx-I activity was found to be dependent on Ca2+, as its substitution by other ionic species as well as the addition of chelating agents significantly reduced its phospholipase activity. In vivo, ColTx-I triggered dose-dependent inflammatory responses, measured using the paw edema model, with an increase in IL-6 levels, systemic and local myotoxicity, characterized by elevated plasma creatine kinase activity. ColTx-I induced a complex series of degenerative events associated with edema, inflammatory infiltrate and skeletal muscle necrosis. These biochemical and functional results suggest that ColTx-I, a myotoxic and inflammatory mediator, plays a relevant role in C. o. lutosus envenomation. Thus, detailed studies on its mechanism of action, such as evaluating the synergism between ColTx-I and other venom components may reveal targets for the development of more specific and effective therapiesCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPFechadoUNIVERSIDADE ESTADUAL DE CAMPINASAlmeida, José Rafael de, 1989-Resende, Letícia Monteiro de, 1990-Marangoni, Sergio, 1951-2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/8783ALMEIDA, José Rafael de; RESENDE, Letícia Monteiro de; MARANGONI, Sergio. Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom. Toxicon: an interdisciplinary journal on the toxins derived from animals, plants and microorganisms. Oxford : Elsevier, 2016. Vol. 117 (July, 2016), p. 1-12. Disponível em: https://hdl.handle.net/20.500.12733/8783. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1168292reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2023-04-25T17:29:00Zoai:https://www.repositorio.unicamp.br/:1168292Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2023-04-25T17:29Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false |
| dc.title.none.fl_str_mv |
Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom |
| title |
Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom |
| spellingShingle |
Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom Almeida, José Rafael de, 1989- Venenos de serpentes Snake venoms Phospholipase A2 Myotoxin Crotalus oreganus lutosus Biological activities Artigo original |
| title_short |
Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom |
| title_full |
Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom |
| title_fullStr |
Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom |
| title_full_unstemmed |
Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom |
| title_sort |
Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom |
| author |
Almeida, José Rafael de, 1989- |
| author_facet |
Almeida, José Rafael de, 1989- Resende, Letícia Monteiro de, 1990- Marangoni, Sergio, 1951- |
| author_role |
author |
| author2 |
Resende, Letícia Monteiro de, 1990- Marangoni, Sergio, 1951- |
| author2_role |
author author |
| dc.contributor.none.fl_str_mv |
UNIVERSIDADE ESTADUAL DE CAMPINAS |
| dc.contributor.author.fl_str_mv |
Almeida, José Rafael de, 1989- Resende, Letícia Monteiro de, 1990- Marangoni, Sergio, 1951- |
| dc.subject.por.fl_str_mv |
Venenos de serpentes Snake venoms Phospholipase A2 Myotoxin Crotalus oreganus lutosus Biological activities Artigo original |
| topic |
Venenos de serpentes Snake venoms Phospholipase A2 Myotoxin Crotalus oreganus lutosus Biological activities Artigo original |
| description |
Agradecimentos: The authors express their gratitude to CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnologico), FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo) for financial support |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12733/8783 ALMEIDA, José Rafael de; RESENDE, Letícia Monteiro de; MARANGONI, Sergio. Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom. Toxicon: an interdisciplinary journal on the toxins derived from animals, plants and microorganisms. Oxford : Elsevier, 2016. Vol. 117 (July, 2016), p. 1-12. Disponível em: https://hdl.handle.net/20.500.12733/8783. Acesso em: 24 mai. 2023. |
| url |
https://hdl.handle.net/20.500.12733/8783 |
| identifier_str_mv |
ALMEIDA, José Rafael de; RESENDE, Letícia Monteiro de; MARANGONI, Sergio. Biochemical and functional studies of ColTx-I, a new myotoxic phospholipase A2 isolated from crotalus oreganus lutosus (great basin rattlesnake) snake venom. Toxicon: an interdisciplinary journal on the toxins derived from animals, plants and microorganisms. Oxford : Elsevier, 2016. Vol. 117 (July, 2016), p. 1-12. Disponível em: https://hdl.handle.net/20.500.12733/8783. Acesso em: 24 mai. 2023. |
| dc.language.iso.fl_str_mv |
Inglês |
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Inglês |
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https://repositorio.unicamp.br/acervo/detalhe/1168292 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório da Produção Científica e Intelectual da Unicamp instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
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Universidade Estadual de Campinas (UNICAMP) |
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UNICAMP |
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UNICAMP |
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Repositório da Produção Científica e Intelectual da Unicamp |
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Repositório da Produção Científica e Intelectual da Unicamp |
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Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP) |
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reposip@unicamp.br |
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