First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Título da fonte: | Repositório da Produção Científica e Intelectual da Unicamp |
Texto Completo: | https://hdl.handle.net/20.500.12733/1979 |
Resumo: | Abstract: Maggot debridement therapy (MDT) consists on the intentional and controlled application of sterilized larvae of the order Diptera on necrotic skin lesions with the purpose of cleaning necrotic tissue and removing pathogenic bacteria. During MDT, a marked antimicrobial activity has been reported in literature specially associated with antibacterial substances from Lucilia sericata (Meigen); however, regarding Cochliomyia macellaria (Fabricius), little is known. This study aimed to evaluate in vitro inhibition of bacterial growth of Pseudomonas aeruginosa and Staphylococcus aureus in contact with excretions and secretions (ES) from C. macellaria larvae. Larval ES were extracted in sterile distilled water and divided in three groups: ES, containing 400 µL of autoclaved ES; ES+BAC, containing 400 µL of autoclaved ES+0.5-µL bacterial inoculum; and CONT-BAC, containing 400 µL of sterile distilled water +0.5 µL of bacterial inoculum. Aliquots of each experimental group were plated by spreading onto Petri dishes. Seedings were made at 0, 1, 2, 4, and 12 h after the extraction of ES. In ES+BAC groups, inhibition of S. aureus was verified between times 1 and 2 h and P. aeruginosa was inhibited between 0 and 4 h. There was no growth observed in any ES group. In the CONT-BAC groups, the number of colonies from time 4 h became countless for S. aureus and decreased for P. aeruginosa. As reported in the literature, we note here that ES have excellent bactericidal activity for both gram-positive and gram-negative bacteria, and this study shows for the first time the action of the bactericidal activity of exosecretions of C. macellaria against S. aureus and P. aeruginosa |
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First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosaStaphylococcus aureusPseudomonas aeruginosaStaphylococcus aureusPseudomonas aeruginosaArtigo originalAbstract: Maggot debridement therapy (MDT) consists on the intentional and controlled application of sterilized larvae of the order Diptera on necrotic skin lesions with the purpose of cleaning necrotic tissue and removing pathogenic bacteria. During MDT, a marked antimicrobial activity has been reported in literature specially associated with antibacterial substances from Lucilia sericata (Meigen); however, regarding Cochliomyia macellaria (Fabricius), little is known. This study aimed to evaluate in vitro inhibition of bacterial growth of Pseudomonas aeruginosa and Staphylococcus aureus in contact with excretions and secretions (ES) from C. macellaria larvae. Larval ES were extracted in sterile distilled water and divided in three groups: ES, containing 400 µL of autoclaved ES; ES+BAC, containing 400 µL of autoclaved ES+0.5-µL bacterial inoculum; and CONT-BAC, containing 400 µL of sterile distilled water +0.5 µL of bacterial inoculum. Aliquots of each experimental group were plated by spreading onto Petri dishes. Seedings were made at 0, 1, 2, 4, and 12 h after the extraction of ES. In ES+BAC groups, inhibition of S. aureus was verified between times 1 and 2 h and P. aeruginosa was inhibited between 0 and 4 h. There was no growth observed in any ES group. In the CONT-BAC groups, the number of colonies from time 4 h became countless for S. aureus and decreased for P. aeruginosa. As reported in the literature, we note here that ES have excellent bactericidal activity for both gram-positive and gram-negative bacteria, and this study shows for the first time the action of the bactericidal activity of exosecretions of C. macellaria against S. aureus and P. aeruginosaFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFechadoUNIVERSIDADE ESTADUAL DE CAMPINASAquino, Marina Ferrari Klemm de, 1993-Nassu, Mariana Prado, 1988-Leite, Domingos da Silva, 1960-Thyssen, Patricia Jacqueline, 1973-2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/1979AQUINO, Marina Ferrari Klemm de et al. First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa. Neotropical entomology. Dordrecht : Springer, 2017. Vol. 46 (Feb., 2017), p. 125–129. Disponível em: https://hdl.handle.net/20.500.12733/1979. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1167505reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2021-12-10T11:47:18Zoai:https://www.repositorio.unicamp.br/:1167505Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2021-12-10T11:47:18Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa |
title |
First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa |
spellingShingle |
First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa Aquino, Marina Ferrari Klemm de, 1993- Staphylococcus aureus Pseudomonas aeruginosa Staphylococcus aureus Pseudomonas aeruginosa Artigo original |
title_short |
First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa |
title_full |
First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa |
title_fullStr |
First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa |
title_full_unstemmed |
First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa |
title_sort |
First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa |
author |
Aquino, Marina Ferrari Klemm de, 1993- |
author_facet |
Aquino, Marina Ferrari Klemm de, 1993- Nassu, Mariana Prado, 1988- Leite, Domingos da Silva, 1960- Thyssen, Patricia Jacqueline, 1973- |
author_role |
author |
author2 |
Nassu, Mariana Prado, 1988- Leite, Domingos da Silva, 1960- Thyssen, Patricia Jacqueline, 1973- |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
UNIVERSIDADE ESTADUAL DE CAMPINAS |
dc.contributor.author.fl_str_mv |
Aquino, Marina Ferrari Klemm de, 1993- Nassu, Mariana Prado, 1988- Leite, Domingos da Silva, 1960- Thyssen, Patricia Jacqueline, 1973- |
dc.subject.por.fl_str_mv |
Staphylococcus aureus Pseudomonas aeruginosa Staphylococcus aureus Pseudomonas aeruginosa Artigo original |
topic |
Staphylococcus aureus Pseudomonas aeruginosa Staphylococcus aureus Pseudomonas aeruginosa Artigo original |
description |
Abstract: Maggot debridement therapy (MDT) consists on the intentional and controlled application of sterilized larvae of the order Diptera on necrotic skin lesions with the purpose of cleaning necrotic tissue and removing pathogenic bacteria. During MDT, a marked antimicrobial activity has been reported in literature specially associated with antibacterial substances from Lucilia sericata (Meigen); however, regarding Cochliomyia macellaria (Fabricius), little is known. This study aimed to evaluate in vitro inhibition of bacterial growth of Pseudomonas aeruginosa and Staphylococcus aureus in contact with excretions and secretions (ES) from C. macellaria larvae. Larval ES were extracted in sterile distilled water and divided in three groups: ES, containing 400 µL of autoclaved ES; ES+BAC, containing 400 µL of autoclaved ES+0.5-µL bacterial inoculum; and CONT-BAC, containing 400 µL of sterile distilled water +0.5 µL of bacterial inoculum. Aliquots of each experimental group were plated by spreading onto Petri dishes. Seedings were made at 0, 1, 2, 4, and 12 h after the extraction of ES. In ES+BAC groups, inhibition of S. aureus was verified between times 1 and 2 h and P. aeruginosa was inhibited between 0 and 4 h. There was no growth observed in any ES group. In the CONT-BAC groups, the number of colonies from time 4 h became countless for S. aureus and decreased for P. aeruginosa. As reported in the literature, we note here that ES have excellent bactericidal activity for both gram-positive and gram-negative bacteria, and this study shows for the first time the action of the bactericidal activity of exosecretions of C. macellaria against S. aureus and P. aeruginosa |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12733/1979 AQUINO, Marina Ferrari Klemm de et al. First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa. Neotropical entomology. Dordrecht : Springer, 2017. Vol. 46 (Feb., 2017), p. 125–129. Disponível em: https://hdl.handle.net/20.500.12733/1979. Acesso em: 24 mai. 2023. |
url |
https://hdl.handle.net/20.500.12733/1979 |
identifier_str_mv |
AQUINO, Marina Ferrari Klemm de et al. First record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) Inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa. Neotropical entomology. Dordrecht : Springer, 2017. Vol. 46 (Feb., 2017), p. 125–129. Disponível em: https://hdl.handle.net/20.500.12733/1979. Acesso em: 24 mai. 2023. |
dc.language.iso.fl_str_mv |
Inglês |
language_invalid_str_mv |
Inglês |
dc.relation.none.fl_str_mv |
https://repositorio.unicamp.br/acervo/detalhe/1167505 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório da Produção Científica e Intelectual da Unicamp instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
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Universidade Estadual de Campinas (UNICAMP) |
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UNICAMP |
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UNICAMP |
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Repositório da Produção Científica e Intelectual da Unicamp |
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Repositório da Produção Científica e Intelectual da Unicamp |
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Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP) |
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reposip@unicamp.br |
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