Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Título da fonte: | Repositório da Produção Científica e Intelectual da Unicamp |
| Texto Completo: | https://hdl.handle.net/20.500.12733/645 |
Resumo: | Agradecimentos: The authors would like to thank Raquel S. Foglio (Hematology and Hemotherapy Center, University of Campinas, São Paulo, Brazil) for the English revision. The authors also thank CNPq, FAPESP, and INCT-Sangue for the financial support. The Hematology and Hemotherapy Center—UNICAMP forms part of the National Institute of Blood, Brazil (INCT de Sangue—CNPq/MCT) |
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Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesionSíndromes mielodisplásicasCélulas - InteraçãoInibidores de proteasesMyelodysplastic syndromesCell interactionProtease inhibitorsArtigo originalAgradecimentos: The authors would like to thank Raquel S. Foglio (Hematology and Hemotherapy Center, University of Campinas, São Paulo, Brazil) for the English revision. The authors also thank CNPq, FAPESP, and INCT-Sangue for the financial support. The Hematology and Hemotherapy Center—UNICAMP forms part of the National Institute of Blood, Brazil (INCT de Sangue—CNPq/MCT)Abstract: Myelodysplastic syndromes (MDS) are clonal disorders involving hematopoietic stem cells (HSC) characterized by ineffective hematopoiesis. In addition to HSC defects, a defective hematopoiesis supporting capacity of mesenchymal stromal cells (MSCs) in the microenvironment niche has been implicated in MDS pathophysiology. The interaction between the dysfunctional MSCs MDS and HSC regulates diverse adhesion-related processes, such as progenitor cell survival, proliferation, differentiation, and self-renewal. As previously reported, a microarray analysis identified serine protease inhibitor kunitz-type 2 (SPINT2), an inhibitor of hepatocyte growth factor (HGF) activation, to be downregulated in MSCs from MDS patients. To define the role of SPINT2 in MDS hematopoietic microenvironment, an analysis of the effect of SPINT2 silencing in MSCs was carried out. We herein reported significantly lower levels of SPINT2 whereas HGF was expressed at higher levels in MSCs from MDS patients compared with healthy controls. SPINT2 underexpression results in an increased expression, production, and secretion of HGF and stromal cell-derived factor 1 (SDF-1) by MSCs. An increased adhesion of normal HSC or malignant cells onto MSCs silenced for SPINT2 was also observed. The altered MSCs adhesion in SPINT2-knockdown cells was correlated with increased CD49b and CD49d expression and with a decrease in CD49e expression. Our results suggest that the SPINT2 underexpression in the MSC from MDS patients is probably involved in the adhesion of progenitors to the bone marrow niche, through an increased HGF and SDF-1 signaling pathwayCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPFechadoUNIVERSIDADE ESTADUAL DE CAMPINASRoversi, Fernanda Marconi, 1981-Lopes, Matheus Rodrigues, 1986-Machado Neto, João Agostinho, 1987-Longhini, Ana Leda Figueiredo, 1978-Duarte, Adriana da Silva Santos, 1971-Baratti, Mariana Ozello, 1980-Palodetto, Bruna, 1987-Corrocher, Flávia Adolfo, 1987-Souza, Fernando Vieira Pericole de, 1981-Campos, Paula de Melo, 1983-Favaro, Patricia Maria Bergamo, 1975-Traina, FabíolaSaad, Sara Teresinha Olalla, 1956-2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/645ROVERSI, Fernanda Marconi et al. Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion. Stem cells and development. New Rochelle, NY : Mary Ann Liebert, 2014. Vol. 23, n. 10 (May, 2014), p. 1109-1120. Disponível em: https://hdl.handle.net/20.500.12733/645. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1231924reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2021-11-16T16:04:52Zoai:https://www.repositorio.unicamp.br/:1231924Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2021-11-16T16:04:52Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false |
| dc.title.none.fl_str_mv |
Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion |
| title |
Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion |
| spellingShingle |
Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion Roversi, Fernanda Marconi, 1981- Síndromes mielodisplásicas Células - Interação Inibidores de proteases Myelodysplastic syndromes Cell interaction Protease inhibitors Artigo original |
| title_short |
Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion |
| title_full |
Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion |
| title_fullStr |
Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion |
| title_full_unstemmed |
Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion |
| title_sort |
Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion |
| author |
Roversi, Fernanda Marconi, 1981- |
| author_facet |
Roversi, Fernanda Marconi, 1981- Lopes, Matheus Rodrigues, 1986- Machado Neto, João Agostinho, 1987- Longhini, Ana Leda Figueiredo, 1978- Duarte, Adriana da Silva Santos, 1971- Baratti, Mariana Ozello, 1980- Palodetto, Bruna, 1987- Corrocher, Flávia Adolfo, 1987- Souza, Fernando Vieira Pericole de, 1981- Campos, Paula de Melo, 1983- Favaro, Patricia Maria Bergamo, 1975- Traina, Fabíola Saad, Sara Teresinha Olalla, 1956- |
| author_role |
author |
| author2 |
Lopes, Matheus Rodrigues, 1986- Machado Neto, João Agostinho, 1987- Longhini, Ana Leda Figueiredo, 1978- Duarte, Adriana da Silva Santos, 1971- Baratti, Mariana Ozello, 1980- Palodetto, Bruna, 1987- Corrocher, Flávia Adolfo, 1987- Souza, Fernando Vieira Pericole de, 1981- Campos, Paula de Melo, 1983- Favaro, Patricia Maria Bergamo, 1975- Traina, Fabíola Saad, Sara Teresinha Olalla, 1956- |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
UNIVERSIDADE ESTADUAL DE CAMPINAS |
| dc.contributor.author.fl_str_mv |
Roversi, Fernanda Marconi, 1981- Lopes, Matheus Rodrigues, 1986- Machado Neto, João Agostinho, 1987- Longhini, Ana Leda Figueiredo, 1978- Duarte, Adriana da Silva Santos, 1971- Baratti, Mariana Ozello, 1980- Palodetto, Bruna, 1987- Corrocher, Flávia Adolfo, 1987- Souza, Fernando Vieira Pericole de, 1981- Campos, Paula de Melo, 1983- Favaro, Patricia Maria Bergamo, 1975- Traina, Fabíola Saad, Sara Teresinha Olalla, 1956- |
| dc.subject.por.fl_str_mv |
Síndromes mielodisplásicas Células - Interação Inibidores de proteases Myelodysplastic syndromes Cell interaction Protease inhibitors Artigo original |
| topic |
Síndromes mielodisplásicas Células - Interação Inibidores de proteases Myelodysplastic syndromes Cell interaction Protease inhibitors Artigo original |
| description |
Agradecimentos: The authors would like to thank Raquel S. Foglio (Hematology and Hemotherapy Center, University of Campinas, São Paulo, Brazil) for the English revision. The authors also thank CNPq, FAPESP, and INCT-Sangue for the financial support. The Hematology and Hemotherapy Center—UNICAMP forms part of the National Institute of Blood, Brazil (INCT de Sangue—CNPq/MCT) |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12733/645 ROVERSI, Fernanda Marconi et al. Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion. Stem cells and development. New Rochelle, NY : Mary Ann Liebert, 2014. Vol. 23, n. 10 (May, 2014), p. 1109-1120. Disponível em: https://hdl.handle.net/20.500.12733/645. Acesso em: 24 mai. 2023. |
| url |
https://hdl.handle.net/20.500.12733/645 |
| identifier_str_mv |
ROVERSI, Fernanda Marconi et al. Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion. Stem cells and development. New Rochelle, NY : Mary Ann Liebert, 2014. Vol. 23, n. 10 (May, 2014), p. 1109-1120. Disponível em: https://hdl.handle.net/20.500.12733/645. Acesso em: 24 mai. 2023. |
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Inglês |
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Inglês |
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https://repositorio.unicamp.br/acervo/detalhe/1231924 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório da Produção Científica e Intelectual da Unicamp instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
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Universidade Estadual de Campinas (UNICAMP) |
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UNICAMP |
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UNICAMP |
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Repositório da Produção Científica e Intelectual da Unicamp |
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Repositório da Produção Científica e Intelectual da Unicamp |
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Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP) |
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reposip@unicamp.br |
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