Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil

Detalhes bibliográficos
Autor(a) principal: Pitassi, Luiza Helena Urso, 1971-
Data de Publicação: 2015
Outros Autores: Drummond, Marina Rovani, 1985-, Lania, Bruno Grosselli, 1987-, Barjas-Castro, Maria Lourdes, Velho, Paulo Eduardo Neves Ferreira, 1966-
Tipo de documento: Artigo
Título da fonte: Repositório da Produção Científica e Intelectual da Unicamp
Texto Completo: https://hdl.handle.net/20.500.12733/9447
Resumo: Agradecimentos: We thank the study patients, Dr. Steven Henriksen and Dr. Dominique Griffon for providing the research infrastructure and support needed at Western University of Health Sciences and Dr. Hannah Mirrashed, John Greenwood, Marizete Salvadego and Fabiana Cristina dos Santos for technical assistance
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spelling Bartonella spp. Bacteremia in Blood donors from Campinas, BrazilBartonellaDoenças transmissíveisBartonellaCommunicable diseasesVinsonii Subsp BerkhoffiiMolecular PrevalenceEgrowth-MediumHenselaeCatsDogsSensitivityQuintanaPatientArtigo de pesquisaAgradecimentos: We thank the study patients, Dr. Steven Henriksen and Dr. Dominique Griffon for providing the research infrastructure and support needed at Western University of Health Sciences and Dr. Hannah Mirrashed, John Greenwood, Marizete Salvadego and Fabiana Cristina dos Santos for technical assistanceAbstract: Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blood donors is unknown, and screening of blood supplies for this pathogen is not routinely performed. We investigated Bartonella spp. prevalence in 500 blood donors from Campinas, Brazil, based on a cross-sectional design. Blood samples were inoculated into an enrichment liquid growth medium and sub-inoculated onto blood agar. Liquid culture samples and Gram-negative isolates were tested using a genus specific ITS PCR with amplicons sequenced for species identification. Bartonella henselae and Bartonella quintana antibodies were assayed by indirect immunofluorescence. B. henselae was isolated from six donors (1.2%). Sixteen donors (3.2%) were Bartonella-PCR positive after culture in liquid or on solid media, with 15 donors infected with B. henselae and one donor infected with Bartonella clarridgeiae. Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively. Serology was not associated with infection, with only three of 16 Bartonella-infected subjects seropositive for B. henselae or B. quintana. Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America. Negative serology does not rule out Bartonella spp. infection in healthy subjects. Using a combination of liquid and solid cultures, PCR, and DNA sequencing, this study documents for the first time that Bartonella spp. bacteremia occurs in asymptomatic blood donors. Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusionsAbertoUNIVERSIDADE ESTADUAL DE CAMPINASPitassi, Luiza Helena Urso, 1971-Drummond, Marina Rovani, 1985-Lania, Bruno Grosselli, 1987-Barjas-Castro, Maria LourdesVelho, Paulo Eduardo Neves Ferreira, 1966-2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/9447PITASSI, Luiza Helena Urso et al. Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil. PLoS neglected tropical diseases. San Francisco, CA : Public Library of Science, 2015. Vol. 9, n. 1 (Jan., 2015), n. art. e0003467. Disponível em: https://hdl.handle.net/20.500.12733/9447. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1168645reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2023-05-15T17:16:53Zoai:https://www.repositorio.unicamp.br/:1168645Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2023-05-15T17:16:53Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil
title Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil
spellingShingle Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil
Pitassi, Luiza Helena Urso, 1971-
Bartonella
Doenças transmissíveis
Bartonella
Communicable diseases
Vinsonii Subsp Berkhoffii
Molecular Prevalence
Egrowth-Medium
Henselae
Cats
Dogs
Sensitivity
Quintana
Patient
Artigo de pesquisa
title_short Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil
title_full Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil
title_fullStr Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil
title_full_unstemmed Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil
title_sort Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil
author Pitassi, Luiza Helena Urso, 1971-
author_facet Pitassi, Luiza Helena Urso, 1971-
Drummond, Marina Rovani, 1985-
Lania, Bruno Grosselli, 1987-
Barjas-Castro, Maria Lourdes
Velho, Paulo Eduardo Neves Ferreira, 1966-
author_role author
author2 Drummond, Marina Rovani, 1985-
Lania, Bruno Grosselli, 1987-
Barjas-Castro, Maria Lourdes
Velho, Paulo Eduardo Neves Ferreira, 1966-
author2_role author
author
author
author
dc.contributor.none.fl_str_mv UNIVERSIDADE ESTADUAL DE CAMPINAS
dc.contributor.author.fl_str_mv Pitassi, Luiza Helena Urso, 1971-
Drummond, Marina Rovani, 1985-
Lania, Bruno Grosselli, 1987-
Barjas-Castro, Maria Lourdes
Velho, Paulo Eduardo Neves Ferreira, 1966-
dc.subject.por.fl_str_mv Bartonella
Doenças transmissíveis
Bartonella
Communicable diseases
Vinsonii Subsp Berkhoffii
Molecular Prevalence
Egrowth-Medium
Henselae
Cats
Dogs
Sensitivity
Quintana
Patient
Artigo de pesquisa
topic Bartonella
Doenças transmissíveis
Bartonella
Communicable diseases
Vinsonii Subsp Berkhoffii
Molecular Prevalence
Egrowth-Medium
Henselae
Cats
Dogs
Sensitivity
Quintana
Patient
Artigo de pesquisa
description Agradecimentos: We thank the study patients, Dr. Steven Henriksen and Dr. Dominique Griffon for providing the research infrastructure and support needed at Western University of Health Sciences and Dr. Hannah Mirrashed, John Greenwood, Marizete Salvadego and Fabiana Cristina dos Santos for technical assistance
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/20.500.12733/9447
PITASSI, Luiza Helena Urso et al. Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil. PLoS neglected tropical diseases. San Francisco, CA : Public Library of Science, 2015. Vol. 9, n. 1 (Jan., 2015), n. art. e0003467. Disponível em: https://hdl.handle.net/20.500.12733/9447. Acesso em: 24 mai. 2023.
url https://hdl.handle.net/20.500.12733/9447
identifier_str_mv PITASSI, Luiza Helena Urso et al. Bartonella spp. Bacteremia in Blood donors from Campinas, Brazil. PLoS neglected tropical diseases. San Francisco, CA : Public Library of Science, 2015. Vol. 9, n. 1 (Jan., 2015), n. art. e0003467. Disponível em: https://hdl.handle.net/20.500.12733/9447. Acesso em: 24 mai. 2023.
dc.language.iso.fl_str_mv Inglês
language_invalid_str_mv Inglês
dc.relation.none.fl_str_mv https://repositorio.unicamp.br/acervo/detalhe/1168645
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório da Produção Científica e Intelectual da Unicamp
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Repositório da Produção Científica e Intelectual da Unicamp
collection Repositório da Produção Científica e Intelectual da Unicamp
repository.name.fl_str_mv Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv reposip@unicamp.br
_version_ 1766887291865792512

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