Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?

Detalhes bibliográficos
Autor(a) principal: Checon, Helio Herminio, 1987-
Data de Publicação: 2017
Outros Autores: Garraffoni, André Rinaldo Senna, 1977-
Tipo de documento: Artigo
Título da fonte: Repositório da Produção Científica e Intelectual da Unicamp
Texto Completo: https://hdl.handle.net/20.500.12733/8822
Resumo: Agradecimentos: The authors would like to thank Dr. Paula Munhoz Omena and Yasmina Shah Esmaeili, Msc, for comments on the first draft of the manuscript and Yasmina Shah Esmaeili, Msc, and Kayla Ann Margaret Wirthwein, who edited the English text. Three anonymous reviewers are also acknowledged for offering suggestions that greatly improved the paper
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spelling Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?GastrotrichaCopépodeTardigradoGastrotrichaCopepodaTardigradaSampling methodsArtigo de pesquisaAgradecimentos: The authors would like to thank Dr. Paula Munhoz Omena and Yasmina Shah Esmaeili, Msc, for comments on the first draft of the manuscript and Yasmina Shah Esmaeili, Msc, and Kayla Ann Margaret Wirthwein, who edited the English text. Three anonymous reviewers are also acknowledged for offering suggestions that greatly improved the paperAbstract: Sampling freshwater biological diversity is a challenge when it comes down to techniques for meiofauna fixation and preservation because this polyphyletic group of taxa is highly diverse. The aim of this study is to test the performance of three anesthetics (CO2, MgCl2 and low temperature) and three fixatives (formaldehyde 4 %; buffered formaldehyde 4 and 70 % ethanol) in the preservation of "soft" (gastrotrichs and rotifers) and "hard" (tardigrades and copepods) freshwater benthic meiofaunal assemblages. Due to these different morphological structures, we expected that treatment performance would vary among taxa in the quality of specimen fixation. Results revealed that the meiofaunal abundances of samples sorted alive or after the treatments with a coupling of anesthetics and fixatives were not different. However, preservation of specimens varied substantially among "soft" and "hard" meiofauna and among treatments. The use of 4 % buffered formaldehyde is highly recommended for freshwater meiofauna, while unbuffered formaldehyde should be avoided. Studies that have "soft" meiofauna as target organisms are recommended to use some type of anesthetic, although it is necessary to use a specific one for each taxon as they respond in different ways to different anestheticsFechadoUNIVERSIDADE ESTADUAL DE CAMPINASChecon, Helio Herminio, 1987-Garraffoni, André Rinaldo Senna, 1977-2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/8822CHECON, Helio Herminio; GARRAFFONI, André Rinaldo Senna. Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna: what is the best method for specimen preservation?. Limnology. Tokyo : Springer, 2017. Vol. 18 (2017), p. 209-218. Disponível em: https://hdl.handle.net/20.500.12733/8822. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1169417reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2023-04-26T16:01:55Zoai:https://www.repositorio.unicamp.br/:1169417Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2023-04-26T16:01:55Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?
title Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?
spellingShingle Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?
Checon, Helio Herminio, 1987-
Gastrotricha
Copépode
Tardigrado
Gastrotricha
Copepoda
Tardigrada
Sampling methods
Artigo de pesquisa
title_short Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?
title_full Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?
title_fullStr Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?
title_full_unstemmed Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?
title_sort Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna : what is the best method for specimen preservation?
author Checon, Helio Herminio, 1987-
author_facet Checon, Helio Herminio, 1987-
Garraffoni, André Rinaldo Senna, 1977-
author_role author
author2 Garraffoni, André Rinaldo Senna, 1977-
author2_role author
dc.contributor.none.fl_str_mv UNIVERSIDADE ESTADUAL DE CAMPINAS
dc.contributor.author.fl_str_mv Checon, Helio Herminio, 1987-
Garraffoni, André Rinaldo Senna, 1977-
dc.subject.por.fl_str_mv Gastrotricha
Copépode
Tardigrado
Gastrotricha
Copepoda
Tardigrada
Sampling methods
Artigo de pesquisa
topic Gastrotricha
Copépode
Tardigrado
Gastrotricha
Copepoda
Tardigrada
Sampling methods
Artigo de pesquisa
description Agradecimentos: The authors would like to thank Dr. Paula Munhoz Omena and Yasmina Shah Esmaeili, Msc, for comments on the first draft of the manuscript and Yasmina Shah Esmaeili, Msc, and Kayla Ann Margaret Wirthwein, who edited the English text. Three anonymous reviewers are also acknowledged for offering suggestions that greatly improved the paper
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/20.500.12733/8822
CHECON, Helio Herminio; GARRAFFONI, André Rinaldo Senna. Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna: what is the best method for specimen preservation?. Limnology. Tokyo : Springer, 2017. Vol. 18 (2017), p. 209-218. Disponível em: https://hdl.handle.net/20.500.12733/8822. Acesso em: 24 mai. 2023.
url https://hdl.handle.net/20.500.12733/8822
identifier_str_mv CHECON, Helio Herminio; GARRAFFONI, André Rinaldo Senna. Evaluating the anesthetization and fixation efficacy of "soft" and "hard" freshwater benthic meiofauna: what is the best method for specimen preservation?. Limnology. Tokyo : Springer, 2017. Vol. 18 (2017), p. 209-218. Disponível em: https://hdl.handle.net/20.500.12733/8822. Acesso em: 24 mai. 2023.
dc.language.iso.fl_str_mv Inglês
language_invalid_str_mv Inglês
dc.relation.none.fl_str_mv https://repositorio.unicamp.br/acervo/detalhe/1169417
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório da Produção Científica e Intelectual da Unicamp
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Repositório da Produção Científica e Intelectual da Unicamp
collection Repositório da Produção Científica e Intelectual da Unicamp
repository.name.fl_str_mv Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv reposip@unicamp.br
_version_ 1766887292865085440

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