In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

Detalhes bibliográficos
Autor(a) principal: Bakhtiar,Ziba
Data de Publicação: 2016
Outros Autores: Mirjalili,Mohammad Hossein, Sonboli,Ali
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000100048
Resumo: Abstract This is the first attempt towards an efficient regeneration protocol for an endangered and valuable medicinal plant, Thymus persicus using in vitro callus induction and indirect organogenesis. Callus induction was performed on MS medium supplemented with different concentrations of NAA and 2,4-D, alone or in combination with BAP and KN. Maximum callus induction (100%) was achieved from internode explants cultured on MS medium fortified with 2.0 mg L-1 NAA and 0.5 mg L-1 KN. The highest frequency of shoot multiplication (96%) was observed with 2.0 mg L-1 BAP+1.0 mg L-1 NAA. The maximum number of rootlets (16.6 ± 1.4) was induced on half-strength MS medium with 0.5 and 1.0 mg L-1 IBA. Rooted plantlets were then successfully grown and acclimatized in the greenhouse with a 70-85% survival rate. The benefits of the protocol described here include all-year-round application, germplasm conservation, suitability for commercial production and also for the biotechnological production of pentacyclic triterpenoids.
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spelling In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plantCallus cultureregenerationorganogenesispentacyclic triterpenoidsAbstract This is the first attempt towards an efficient regeneration protocol for an endangered and valuable medicinal plant, Thymus persicus using in vitro callus induction and indirect organogenesis. Callus induction was performed on MS medium supplemented with different concentrations of NAA and 2,4-D, alone or in combination with BAP and KN. Maximum callus induction (100%) was achieved from internode explants cultured on MS medium fortified with 2.0 mg L-1 NAA and 0.5 mg L-1 KN. The highest frequency of shoot multiplication (96%) was observed with 2.0 mg L-1 BAP+1.0 mg L-1 NAA. The maximum number of rootlets (16.6 ± 1.4) was induced on half-strength MS medium with 0.5 and 1.0 mg L-1 IBA. Rooted plantlets were then successfully grown and acclimatized in the greenhouse with a 70-85% survival rate. The benefits of the protocol described here include all-year-round application, germplasm conservation, suitability for commercial production and also for the biotechnological production of pentacyclic triterpenoids.Crop Breeding and Applied Biotechnology2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000100048Crop Breeding and Applied Biotechnology v.16 n.1 2016reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332016v16n1a8info:eu-repo/semantics/openAccessBakhtiar,ZibaMirjalili,Mohammad HosseinSonboli,Alieng2016-04-19T00:00:00Zoai:scielo:S1984-70332016000100048Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2016-04-19T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant
title In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant
spellingShingle In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant
Bakhtiar,Ziba
Callus culture
regeneration
organogenesis
pentacyclic triterpenoids
title_short In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant
title_full In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant
title_fullStr In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant
title_full_unstemmed In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant
title_sort In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant
author Bakhtiar,Ziba
author_facet Bakhtiar,Ziba
Mirjalili,Mohammad Hossein
Sonboli,Ali
author_role author
author2 Mirjalili,Mohammad Hossein
Sonboli,Ali
author2_role author
author
dc.contributor.author.fl_str_mv Bakhtiar,Ziba
Mirjalili,Mohammad Hossein
Sonboli,Ali
dc.subject.por.fl_str_mv Callus culture
regeneration
organogenesis
pentacyclic triterpenoids
topic Callus culture
regeneration
organogenesis
pentacyclic triterpenoids
description Abstract This is the first attempt towards an efficient regeneration protocol for an endangered and valuable medicinal plant, Thymus persicus using in vitro callus induction and indirect organogenesis. Callus induction was performed on MS medium supplemented with different concentrations of NAA and 2,4-D, alone or in combination with BAP and KN. Maximum callus induction (100%) was achieved from internode explants cultured on MS medium fortified with 2.0 mg L-1 NAA and 0.5 mg L-1 KN. The highest frequency of shoot multiplication (96%) was observed with 2.0 mg L-1 BAP+1.0 mg L-1 NAA. The maximum number of rootlets (16.6 ± 1.4) was induced on half-strength MS medium with 0.5 and 1.0 mg L-1 IBA. Rooted plantlets were then successfully grown and acclimatized in the greenhouse with a 70-85% survival rate. The benefits of the protocol described here include all-year-round application, germplasm conservation, suitability for commercial production and also for the biotechnological production of pentacyclic triterpenoids.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000100048
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000100048
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332016v16n1a8
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.16 n.1 2016
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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