Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil

Detalhes bibliográficos
Autor(a) principal: Cordeiro,Sandra Zorat
Data de Publicação: 2014
Outros Autores: Simas,Naomi Kato, Henriques,Anaize Borges, Sato,Alice
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000200007
Resumo: Mandevilla guanabarica is an endemic plant from Brazil, with pharmacological and ornamental potential, both unexplored. This study established the best culture medium for in vitro plant maintenance, efficient protocol for its regeneration, and callogenesis from different explants excised from in vitro-grown plants. Woody plant medium with double boron concentration (WPM B) plus 2.27 µM thidiazuron or 0.49 µM 2-isopentenyladenine provided multiplication rates higher than 1:6. Shoots were 100% rooted on WPM B. After acclimatization, plants showed 83% survival. For callogenesis, the use of MS media supplemented with high concentrations of picloram or 2,4-dichlorophenoxyacetic acid produced, respectively, friable or compact non-morphogenic calluses from different types of explants. This micropropagation protocol allows the production of seedlings of M. guanabarica for ornamental or commercial uses, and for conservation purposes; calluses can be used to establish suspension cultures in prospecting for bioactive compounds.
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spelling Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from BrazilMorphogenesistissue cultureplant regenerationcallus inductionMandevilla guanabarica is an endemic plant from Brazil, with pharmacological and ornamental potential, both unexplored. This study established the best culture medium for in vitro plant maintenance, efficient protocol for its regeneration, and callogenesis from different explants excised from in vitro-grown plants. Woody plant medium with double boron concentration (WPM B) plus 2.27 µM thidiazuron or 0.49 µM 2-isopentenyladenine provided multiplication rates higher than 1:6. Shoots were 100% rooted on WPM B. After acclimatization, plants showed 83% survival. For callogenesis, the use of MS media supplemented with high concentrations of picloram or 2,4-dichlorophenoxyacetic acid produced, respectively, friable or compact non-morphogenic calluses from different types of explants. This micropropagation protocol allows the production of seedlings of M. guanabarica for ornamental or commercial uses, and for conservation purposes; calluses can be used to establish suspension cultures in prospecting for bioactive compounds.Crop Breeding and Applied Biotechnology2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000200007Crop Breeding and Applied Biotechnology v.14 n.2 2014reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332014v14n2a19info:eu-repo/semantics/openAccessCordeiro,Sandra ZoratSimas,Naomi KatoHenriques,Anaize BorgesSato,Aliceeng2014-06-25T00:00:00Zoai:scielo:S1984-70332014000200007Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2014-06-25T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil
title Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil
spellingShingle Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil
Cordeiro,Sandra Zorat
Morphogenesis
tissue culture
plant regeneration
callus induction
title_short Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil
title_full Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil
title_fullStr Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil
title_full_unstemmed Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil
title_sort Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil
author Cordeiro,Sandra Zorat
author_facet Cordeiro,Sandra Zorat
Simas,Naomi Kato
Henriques,Anaize Borges
Sato,Alice
author_role author
author2 Simas,Naomi Kato
Henriques,Anaize Borges
Sato,Alice
author2_role author
author
author
dc.contributor.author.fl_str_mv Cordeiro,Sandra Zorat
Simas,Naomi Kato
Henriques,Anaize Borges
Sato,Alice
dc.subject.por.fl_str_mv Morphogenesis
tissue culture
plant regeneration
callus induction
topic Morphogenesis
tissue culture
plant regeneration
callus induction
description Mandevilla guanabarica is an endemic plant from Brazil, with pharmacological and ornamental potential, both unexplored. This study established the best culture medium for in vitro plant maintenance, efficient protocol for its regeneration, and callogenesis from different explants excised from in vitro-grown plants. Woody plant medium with double boron concentration (WPM B) plus 2.27 µM thidiazuron or 0.49 µM 2-isopentenyladenine provided multiplication rates higher than 1:6. Shoots were 100% rooted on WPM B. After acclimatization, plants showed 83% survival. For callogenesis, the use of MS media supplemented with high concentrations of picloram or 2,4-dichlorophenoxyacetic acid produced, respectively, friable or compact non-morphogenic calluses from different types of explants. This micropropagation protocol allows the production of seedlings of M. guanabarica for ornamental or commercial uses, and for conservation purposes; calluses can be used to establish suspension cultures in prospecting for bioactive compounds.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000200007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000200007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332014v14n2a19
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.14 n.2 2014
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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