DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000300001 |
Resumo: | Forty-seven Japanese plum (Prunus salicina) cultivars were genotyped with eight microsatellite markers, aiming at obtaining the DNA fingerprinting profiling, distinguishing and characterizing a representative set of Japanese plum cultivars. The eight SSR loci amplified 104 alleles (8 to 21 alleles per locus, mean 13). Polymorphism Information Content (PIC) ranged from 0.680 to 0.886 (mean 0.803). The observed heterozigozity (Ho) ranged from 0.529 to 0.915 (mean 0.770). Probability of Identity (I) of each locus ranged from 0.019 to 0.113 (mean 0.054). The combined Probability of Identity was 2.66 x 1011, and the Power of Exclusion of the eight loci was 99.99976%. 57 out of 104 alleles showed frequency lower than 0.05. These low allele frequencies contributed to raise the distinguish ability of plum cultivars. These results will contribute, as excellent descriptors, to select parental for crossings, to perform early identification of segregating clones with potential to be cultivars, and to protect the cultivars. |
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Crop Breeding and Applied Biotechnology |
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DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markersCultivar protectioncultivar identificationgenetic similarity/relationshipSSR markersForty-seven Japanese plum (Prunus salicina) cultivars were genotyped with eight microsatellite markers, aiming at obtaining the DNA fingerprinting profiling, distinguishing and characterizing a representative set of Japanese plum cultivars. The eight SSR loci amplified 104 alleles (8 to 21 alleles per locus, mean 13). Polymorphism Information Content (PIC) ranged from 0.680 to 0.886 (mean 0.803). The observed heterozigozity (Ho) ranged from 0.529 to 0.915 (mean 0.770). Probability of Identity (I) of each locus ranged from 0.019 to 0.113 (mean 0.054). The combined Probability of Identity was 2.66 x 1011, and the Power of Exclusion of the eight loci was 99.99976%. 57 out of 104 alleles showed frequency lower than 0.05. These low allele frequencies contributed to raise the distinguish ability of plum cultivars. These results will contribute, as excellent descriptors, to select parental for crossings, to perform early identification of segregating clones with potential to be cultivars, and to protect the cultivars.Crop Breeding and Applied Biotechnology2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000300001Crop Breeding and Applied Biotechnology v.14 n.3 2014reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332014v14n3a21info:eu-repo/semantics/openAccessKlabunde,Gustavo Henrique FerreroDalbó,Marco AntonioNodari,Rubens Onofreeng2014-11-25T00:00:00Zoai:scielo:S1984-70332014000300001Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2014-11-25T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers |
title |
DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers |
spellingShingle |
DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers Klabunde,Gustavo Henrique Ferrero Cultivar protection cultivar identification genetic similarity/relationship SSR markers |
title_short |
DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers |
title_full |
DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers |
title_fullStr |
DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers |
title_full_unstemmed |
DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers |
title_sort |
DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers |
author |
Klabunde,Gustavo Henrique Ferrero |
author_facet |
Klabunde,Gustavo Henrique Ferrero Dalbó,Marco Antonio Nodari,Rubens Onofre |
author_role |
author |
author2 |
Dalbó,Marco Antonio Nodari,Rubens Onofre |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Klabunde,Gustavo Henrique Ferrero Dalbó,Marco Antonio Nodari,Rubens Onofre |
dc.subject.por.fl_str_mv |
Cultivar protection cultivar identification genetic similarity/relationship SSR markers |
topic |
Cultivar protection cultivar identification genetic similarity/relationship SSR markers |
description |
Forty-seven Japanese plum (Prunus salicina) cultivars were genotyped with eight microsatellite markers, aiming at obtaining the DNA fingerprinting profiling, distinguishing and characterizing a representative set of Japanese plum cultivars. The eight SSR loci amplified 104 alleles (8 to 21 alleles per locus, mean 13). Polymorphism Information Content (PIC) ranged from 0.680 to 0.886 (mean 0.803). The observed heterozigozity (Ho) ranged from 0.529 to 0.915 (mean 0.770). Probability of Identity (I) of each locus ranged from 0.019 to 0.113 (mean 0.054). The combined Probability of Identity was 2.66 x 1011, and the Power of Exclusion of the eight loci was 99.99976%. 57 out of 104 alleles showed frequency lower than 0.05. These low allele frequencies contributed to raise the distinguish ability of plum cultivars. These results will contribute, as excellent descriptors, to select parental for crossings, to perform early identification of segregating clones with potential to be cultivars, and to protect the cultivars. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000300001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332014v14n3a21 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.14 n.3 2014 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209186713960448 |