Chromosome duplication in Lolium multiflorum Lam.

Detalhes bibliográficos
Autor(a) principal: Pereira,Roselaine Cristina
Data de Publicação: 2014
Outros Autores: Ferreira,Marco Túlio Mendes, Davide,Lisete Chamma, Pasqual,Moacir, Mittelmann,Andréa, Techio,Vânia Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000400007
Resumo: Artificial chromosome duplication of diploid genotypes of Lolium multiflorum (2n=2x=14) is worthy to breeding, and aims to increase the expression of traits with agronomic interest. The purpose of this study was to obtain polyploid plants of L. multiflorum from local diploid populations in order to exploit adaptation and future verification of the effects of polyploidy in agronomic traits. Seedlings were immersed in different colchicine solutions for an exposure time of 3h and 24h. Ploidy determination was made by the DNA content and certified by chromosomes counts. The plants confirmed as tetraploids were placed in a greenhouse, and, at flowering, pollen viability was evaluated, and seeds were harvested to assess the stability of the progenies. The percentage of polyploids obtained was 20%. Pollen viability of the tetraploids generated ranged from 58% to 69%. The tetraploid plants obtained in the experiment generated 164 progenies, of which 109 presented DNA content compatible with the tetraploid level, showing stability of chromosome duplication in the filial generation.
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spelling Chromosome duplication in Lolium multiflorum Lam.Polyploidizationtetraploidforage grassplant breedingArtificial chromosome duplication of diploid genotypes of Lolium multiflorum (2n=2x=14) is worthy to breeding, and aims to increase the expression of traits with agronomic interest. The purpose of this study was to obtain polyploid plants of L. multiflorum from local diploid populations in order to exploit adaptation and future verification of the effects of polyploidy in agronomic traits. Seedlings were immersed in different colchicine solutions for an exposure time of 3h and 24h. Ploidy determination was made by the DNA content and certified by chromosomes counts. The plants confirmed as tetraploids were placed in a greenhouse, and, at flowering, pollen viability was evaluated, and seeds were harvested to assess the stability of the progenies. The percentage of polyploids obtained was 20%. Pollen viability of the tetraploids generated ranged from 58% to 69%. The tetraploid plants obtained in the experiment generated 164 progenies, of which 109 presented DNA content compatible with the tetraploid level, showing stability of chromosome duplication in the filial generation.Crop Breeding and Applied Biotechnology2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000400007Crop Breeding and Applied Biotechnology v.14 n.4 2014reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332014v14n4n39info:eu-repo/semantics/openAccessPereira,Roselaine CristinaFerreira,Marco Túlio MendesDavide,Lisete ChammaPasqual,MoacirMittelmann,AndréaTechio,Vânia Helenaeng2014-12-09T00:00:00Zoai:scielo:S1984-70332014000400007Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2014-12-09T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Chromosome duplication in Lolium multiflorum Lam.
title Chromosome duplication in Lolium multiflorum Lam.
spellingShingle Chromosome duplication in Lolium multiflorum Lam.
Pereira,Roselaine Cristina
Polyploidization
tetraploid
forage grass
plant breeding
title_short Chromosome duplication in Lolium multiflorum Lam.
title_full Chromosome duplication in Lolium multiflorum Lam.
title_fullStr Chromosome duplication in Lolium multiflorum Lam.
title_full_unstemmed Chromosome duplication in Lolium multiflorum Lam.
title_sort Chromosome duplication in Lolium multiflorum Lam.
author Pereira,Roselaine Cristina
author_facet Pereira,Roselaine Cristina
Ferreira,Marco Túlio Mendes
Davide,Lisete Chamma
Pasqual,Moacir
Mittelmann,Andréa
Techio,Vânia Helena
author_role author
author2 Ferreira,Marco Túlio Mendes
Davide,Lisete Chamma
Pasqual,Moacir
Mittelmann,Andréa
Techio,Vânia Helena
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira,Roselaine Cristina
Ferreira,Marco Túlio Mendes
Davide,Lisete Chamma
Pasqual,Moacir
Mittelmann,Andréa
Techio,Vânia Helena
dc.subject.por.fl_str_mv Polyploidization
tetraploid
forage grass
plant breeding
topic Polyploidization
tetraploid
forage grass
plant breeding
description Artificial chromosome duplication of diploid genotypes of Lolium multiflorum (2n=2x=14) is worthy to breeding, and aims to increase the expression of traits with agronomic interest. The purpose of this study was to obtain polyploid plants of L. multiflorum from local diploid populations in order to exploit adaptation and future verification of the effects of polyploidy in agronomic traits. Seedlings were immersed in different colchicine solutions for an exposure time of 3h and 24h. Ploidy determination was made by the DNA content and certified by chromosomes counts. The plants confirmed as tetraploids were placed in a greenhouse, and, at flowering, pollen viability was evaluated, and seeds were harvested to assess the stability of the progenies. The percentage of polyploids obtained was 20%. Pollen viability of the tetraploids generated ranged from 58% to 69%. The tetraploid plants obtained in the experiment generated 164 progenies, of which 109 presented DNA content compatible with the tetraploid level, showing stability of chromosome duplication in the filial generation.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000400007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332014000400007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332014v14n4n39
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.14 n.4 2014
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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