MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stress

Detalhes bibliográficos
Autor(a) principal: Silva,Jóice de Oliveira Leite
Data de Publicação: 2021
Outros Autores: Silva,Renan Gonçalves da, Nogueira,Lucas de Faria, Zingaretti,Sonia Marli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100204
Resumo: Abstract The agricultural yield of sugarcane (Saccharum spp.) is influenced by various abiotic stresses, including aluminum toxicity (Al3+). MicroRNAs (miRNAs) play a role in plant tolerance to such stresses by modulating the expression of several important target genes involved in plant growth. This study investigated the possible tolerance mechanisms of two sugarcane genotypes (CTC-2 and RB855453) under Al3+ stress through miRNA expression profiles and in silico analysis of target genes. The expression data obtained using RT-qPCR and co-expression network analysis identified two possible regulatory mechanisms in the tolerant genotype (CTC-2) under Al3+ stress. miR395 was involved in Al3+ detoxification, whereas miR160, miR6225-5p, and miR167 participated in the process of lateral root formation, conferring tolerance to the genotype. These findings might be useful for biotechnological strategies that aim for miRNA silencing or gene overexpression and provide subsidies for future genetic improvement programs aimed at the development of abiotic stress-tolerant sugarcane genotypes.
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spelling MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stressAluminum toxicityco-expression networkRT-qPCRSaccharumsmall RNAsAbstract The agricultural yield of sugarcane (Saccharum spp.) is influenced by various abiotic stresses, including aluminum toxicity (Al3+). MicroRNAs (miRNAs) play a role in plant tolerance to such stresses by modulating the expression of several important target genes involved in plant growth. This study investigated the possible tolerance mechanisms of two sugarcane genotypes (CTC-2 and RB855453) under Al3+ stress through miRNA expression profiles and in silico analysis of target genes. The expression data obtained using RT-qPCR and co-expression network analysis identified two possible regulatory mechanisms in the tolerant genotype (CTC-2) under Al3+ stress. miR395 was involved in Al3+ detoxification, whereas miR160, miR6225-5p, and miR167 participated in the process of lateral root formation, conferring tolerance to the genotype. These findings might be useful for biotechnological strategies that aim for miRNA silencing or gene overexpression and provide subsidies for future genetic improvement programs aimed at the development of abiotic stress-tolerant sugarcane genotypes.Crop Breeding and Applied Biotechnology2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100204Crop Breeding and Applied Biotechnology v.21 n.1 2021reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332021v21n1a5info:eu-repo/semantics/openAccessSilva,Jóice de Oliveira LeiteSilva,Renan Gonçalves daNogueira,Lucas de FariaZingaretti,Sonia Marlieng2021-02-09T00:00:00Zoai:scielo:S1984-70332021000100204Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2021-02-09T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stress
title MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stress
spellingShingle MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stress
Silva,Jóice de Oliveira Leite
Aluminum toxicity
co-expression network
RT-qPCR
Saccharum
small RNAs
title_short MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stress
title_full MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stress
title_fullStr MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stress
title_full_unstemmed MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stress
title_sort MicroRNAs regulate tolerance mechanisms in sugarcane (Saccharum spp.) under aluminum stress
author Silva,Jóice de Oliveira Leite
author_facet Silva,Jóice de Oliveira Leite
Silva,Renan Gonçalves da
Nogueira,Lucas de Faria
Zingaretti,Sonia Marli
author_role author
author2 Silva,Renan Gonçalves da
Nogueira,Lucas de Faria
Zingaretti,Sonia Marli
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva,Jóice de Oliveira Leite
Silva,Renan Gonçalves da
Nogueira,Lucas de Faria
Zingaretti,Sonia Marli
dc.subject.por.fl_str_mv Aluminum toxicity
co-expression network
RT-qPCR
Saccharum
small RNAs
topic Aluminum toxicity
co-expression network
RT-qPCR
Saccharum
small RNAs
description Abstract The agricultural yield of sugarcane (Saccharum spp.) is influenced by various abiotic stresses, including aluminum toxicity (Al3+). MicroRNAs (miRNAs) play a role in plant tolerance to such stresses by modulating the expression of several important target genes involved in plant growth. This study investigated the possible tolerance mechanisms of two sugarcane genotypes (CTC-2 and RB855453) under Al3+ stress through miRNA expression profiles and in silico analysis of target genes. The expression data obtained using RT-qPCR and co-expression network analysis identified two possible regulatory mechanisms in the tolerant genotype (CTC-2) under Al3+ stress. miR395 was involved in Al3+ detoxification, whereas miR160, miR6225-5p, and miR167 participated in the process of lateral root formation, conferring tolerance to the genotype. These findings might be useful for biotechnological strategies that aim for miRNA silencing or gene overexpression and provide subsidies for future genetic improvement programs aimed at the development of abiotic stress-tolerant sugarcane genotypes.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100204
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100204
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332021v21n1a5
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.21 n.1 2021
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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