Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8

Detalhes bibliográficos
Autor(a) principal: Pinheiro,Thiago Martins
Data de Publicação: 2012
Outros Autores: Araújo,Leila Garcês de, Silva-Lobo,Valácia Leme da, Prabhu,Anne Sitarama, Filippi,Marta Cristina de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332012000300001
Resumo: The rice cultivar Cica-8 exhibit differential reaction to several pathotypes of Magnaporthe oryzae. The objective of the present investigation was to determine the number of alleles involved in the expression of resistance to leaf blast and identify microsatellite markers linked to these alleles. A cross between cultivar Metica-1 and Cica-8 susceptible and resistant, respectively, to pathotype IB-1 (Py1049) was made to obtain F1, F2, BC1:1 and BC1:2 progenies. Greenhouse tests for leaf blast reaction showed that resistance is controlled by a monogenic dominant gene. For testing microsatellite markers, DNA of both resistant and susceptible parents and F1 and F2 populations was extracted. As expected for single dominant gene the F2 populations segregated at a ratio of 3:1. Of the 11 microsatellite markers tested, one marker RM 7102 was found to be closely linked to the resistant allele at a distance of 2.7 cM, in the cultivar Cica-8 to pathotype IB-1.
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spelling Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8Oryza sativaPyricularia oryzaeDNA markersmarker assisted selectionThe rice cultivar Cica-8 exhibit differential reaction to several pathotypes of Magnaporthe oryzae. The objective of the present investigation was to determine the number of alleles involved in the expression of resistance to leaf blast and identify microsatellite markers linked to these alleles. A cross between cultivar Metica-1 and Cica-8 susceptible and resistant, respectively, to pathotype IB-1 (Py1049) was made to obtain F1, F2, BC1:1 and BC1:2 progenies. Greenhouse tests for leaf blast reaction showed that resistance is controlled by a monogenic dominant gene. For testing microsatellite markers, DNA of both resistant and susceptible parents and F1 and F2 populations was extracted. As expected for single dominant gene the F2 populations segregated at a ratio of 3:1. Of the 11 microsatellite markers tested, one marker RM 7102 was found to be closely linked to the resistant allele at a distance of 2.7 cM, in the cultivar Cica-8 to pathotype IB-1.Crop Breeding and Applied Biotechnology2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332012000300001Crop Breeding and Applied Biotechnology v.12 n.3 2012reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/S1984-70332012000300001info:eu-repo/semantics/openAccessPinheiro,Thiago MartinsAraújo,Leila Garcês deSilva-Lobo,Valácia Leme daPrabhu,Anne SitaramaFilippi,Marta Cristina deeng2012-09-25T00:00:00Zoai:scielo:S1984-70332012000300001Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2012-09-25T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8
title Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8
spellingShingle Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8
Pinheiro,Thiago Martins
Oryza sativa
Pyricularia oryzae
DNA markers
marker assisted selection
title_short Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8
title_full Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8
title_fullStr Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8
title_full_unstemmed Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8
title_sort Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8
author Pinheiro,Thiago Martins
author_facet Pinheiro,Thiago Martins
Araújo,Leila Garcês de
Silva-Lobo,Valácia Leme da
Prabhu,Anne Sitarama
Filippi,Marta Cristina de
author_role author
author2 Araújo,Leila Garcês de
Silva-Lobo,Valácia Leme da
Prabhu,Anne Sitarama
Filippi,Marta Cristina de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pinheiro,Thiago Martins
Araújo,Leila Garcês de
Silva-Lobo,Valácia Leme da
Prabhu,Anne Sitarama
Filippi,Marta Cristina de
dc.subject.por.fl_str_mv Oryza sativa
Pyricularia oryzae
DNA markers
marker assisted selection
topic Oryza sativa
Pyricularia oryzae
DNA markers
marker assisted selection
description The rice cultivar Cica-8 exhibit differential reaction to several pathotypes of Magnaporthe oryzae. The objective of the present investigation was to determine the number of alleles involved in the expression of resistance to leaf blast and identify microsatellite markers linked to these alleles. A cross between cultivar Metica-1 and Cica-8 susceptible and resistant, respectively, to pathotype IB-1 (Py1049) was made to obtain F1, F2, BC1:1 and BC1:2 progenies. Greenhouse tests for leaf blast reaction showed that resistance is controlled by a monogenic dominant gene. For testing microsatellite markers, DNA of both resistant and susceptible parents and F1 and F2 populations was extracted. As expected for single dominant gene the F2 populations segregated at a ratio of 3:1. Of the 11 microsatellite markers tested, one marker RM 7102 was found to be closely linked to the resistant allele at a distance of 2.7 cM, in the cultivar Cica-8 to pathotype IB-1.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332012000300001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332012000300001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-70332012000300001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.12 n.3 2012
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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