Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Colégio Brasileiro de Cirurgiões |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912016000400235 |
Resumo: | ABSTRACT Objective: to evaluate the influence of Duodenal reflux in histological changes of the gastric mucosa of rats infected with Helicobacter pylori submitted to pyloroplasty. Methods: after two weeks of acclimation, we infected 30 male Wistar rats with Helicobacter pylori. We randomly divided them into three groups: one submitted to pyloroplasty, another to partial gastrectomy and the third, only infected, was not operated. After six months of surgery, euthanasia was carried out. Gastric fragments were studied by light microscopy to count the number of H. pylori, and to observe the histological changes (gastritis, metaplasia, dysplasia and neoplasia). We confirmed these changes by immunohistochemistry using the molecular markers PCNA and TGF-beta. Results: the animals submitted to pyloroplasty had higher percentage of colonization by H. pylori (median=58.5; gastrectomy=16.5; control=14.5). There was a positive correlation between the amount of H. pylori and the occurrence of chronic gastritis present in the antral fragments. Neoplasia occurred in 40% of rats from the group submitted to pyloroplasty. The staining with PCNA and TGF-ß confirmed the histopathological changes visualized by optical microscopy. Conclusions: the antral region was the one with the highest concentration of H. pylori, regardless of the group. There was a positive correlation between the appearance of benign disorders (chronic gastritis, metaplasia, dysplasia) and cancer in mice infected with H. pylori submitted to pyloroplasty. |
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Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pyloriStomach Neoplasms. Helicobacter pylori. Duodenogastric Reflux. RatsWistar.ABSTRACT Objective: to evaluate the influence of Duodenal reflux in histological changes of the gastric mucosa of rats infected with Helicobacter pylori submitted to pyloroplasty. Methods: after two weeks of acclimation, we infected 30 male Wistar rats with Helicobacter pylori. We randomly divided them into three groups: one submitted to pyloroplasty, another to partial gastrectomy and the third, only infected, was not operated. After six months of surgery, euthanasia was carried out. Gastric fragments were studied by light microscopy to count the number of H. pylori, and to observe the histological changes (gastritis, metaplasia, dysplasia and neoplasia). We confirmed these changes by immunohistochemistry using the molecular markers PCNA and TGF-beta. Results: the animals submitted to pyloroplasty had higher percentage of colonization by H. pylori (median=58.5; gastrectomy=16.5; control=14.5). There was a positive correlation between the amount of H. pylori and the occurrence of chronic gastritis present in the antral fragments. Neoplasia occurred in 40% of rats from the group submitted to pyloroplasty. The staining with PCNA and TGF-ß confirmed the histopathological changes visualized by optical microscopy. Conclusions: the antral region was the one with the highest concentration of H. pylori, regardless of the group. There was a positive correlation between the appearance of benign disorders (chronic gastritis, metaplasia, dysplasia) and cancer in mice infected with H. pylori submitted to pyloroplasty.Colégio Brasileiro de Cirurgiões2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912016000400235Revista do Colégio Brasileiro de Cirurgiões v.43 n.4 2016reponame:Revista do Colégio Brasileiro de Cirurgiõesinstname:Colégio Brasileiro de Cirurgiões (CBC)instacron:CBC10.1590/0100-69912016004003info:eu-repo/semantics/openAccessARAUJO,JOSÉ CARLOS RIBEIRO DECARVALHO,JORGE JOSÉ DESERRA,HUMBERTO OLIVEIRAeng2016-09-16T00:00:00Zoai:scielo:S0100-69912016000400235Revistahttp://www.scielo.br/rcbcONGhttps://old.scielo.br/oai/scielo-oai.php||revistacbc@cbc.org.br1809-45460100-6991opendoar:2016-09-16T00:00Revista do Colégio Brasileiro de Cirurgiões - Colégio Brasileiro de Cirurgiões (CBC)false |
dc.title.none.fl_str_mv |
Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori |
title |
Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori |
spellingShingle |
Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori ARAUJO,JOSÉ CARLOS RIBEIRO DE Stomach Neoplasms. Helicobacter pylori. Duodenogastric Reflux. Rats Wistar. |
title_short |
Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori |
title_full |
Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori |
title_fullStr |
Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori |
title_full_unstemmed |
Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori |
title_sort |
Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori |
author |
ARAUJO,JOSÉ CARLOS RIBEIRO DE |
author_facet |
ARAUJO,JOSÉ CARLOS RIBEIRO DE CARVALHO,JORGE JOSÉ DE SERRA,HUMBERTO OLIVEIRA |
author_role |
author |
author2 |
CARVALHO,JORGE JOSÉ DE SERRA,HUMBERTO OLIVEIRA |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
ARAUJO,JOSÉ CARLOS RIBEIRO DE CARVALHO,JORGE JOSÉ DE SERRA,HUMBERTO OLIVEIRA |
dc.subject.por.fl_str_mv |
Stomach Neoplasms. Helicobacter pylori. Duodenogastric Reflux. Rats Wistar. |
topic |
Stomach Neoplasms. Helicobacter pylori. Duodenogastric Reflux. Rats Wistar. |
description |
ABSTRACT Objective: to evaluate the influence of Duodenal reflux in histological changes of the gastric mucosa of rats infected with Helicobacter pylori submitted to pyloroplasty. Methods: after two weeks of acclimation, we infected 30 male Wistar rats with Helicobacter pylori. We randomly divided them into three groups: one submitted to pyloroplasty, another to partial gastrectomy and the third, only infected, was not operated. After six months of surgery, euthanasia was carried out. Gastric fragments were studied by light microscopy to count the number of H. pylori, and to observe the histological changes (gastritis, metaplasia, dysplasia and neoplasia). We confirmed these changes by immunohistochemistry using the molecular markers PCNA and TGF-beta. Results: the animals submitted to pyloroplasty had higher percentage of colonization by H. pylori (median=58.5; gastrectomy=16.5; control=14.5). There was a positive correlation between the amount of H. pylori and the occurrence of chronic gastritis present in the antral fragments. Neoplasia occurred in 40% of rats from the group submitted to pyloroplasty. The staining with PCNA and TGF-ß confirmed the histopathological changes visualized by optical microscopy. Conclusions: the antral region was the one with the highest concentration of H. pylori, regardless of the group. There was a positive correlation between the appearance of benign disorders (chronic gastritis, metaplasia, dysplasia) and cancer in mice infected with H. pylori submitted to pyloroplasty. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912016000400235 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912016000400235 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0100-69912016004003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Cirurgiões |
publisher.none.fl_str_mv |
Colégio Brasileiro de Cirurgiões |
dc.source.none.fl_str_mv |
Revista do Colégio Brasileiro de Cirurgiões v.43 n.4 2016 reponame:Revista do Colégio Brasileiro de Cirurgiões instname:Colégio Brasileiro de Cirurgiões (CBC) instacron:CBC |
instname_str |
Colégio Brasileiro de Cirurgiões (CBC) |
instacron_str |
CBC |
institution |
CBC |
reponame_str |
Revista do Colégio Brasileiro de Cirurgiões |
collection |
Revista do Colégio Brasileiro de Cirurgiões |
repository.name.fl_str_mv |
Revista do Colégio Brasileiro de Cirurgiões - Colégio Brasileiro de Cirurgiões (CBC) |
repository.mail.fl_str_mv |
||revistacbc@cbc.org.br |
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1754209213555408896 |