Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Colégio Brasileiro de Cirurgiões |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912014000100049 |
Resumo: | OBJECTIVE: to compare the effects of low intensity laser therapy on in vitro bacterial growth and in vivo in infected wounds, and to analyze the effectiveness of the AsGa Laser technology in in vivo wound infections. METHODS: in vitro: Staphylococcus aureus were incubated on blood agar plates, half of them being irradiated with 904 nm wavelength laser and dose of 3J/cm2 daily for seven days. In vivo: 32 male Wistar rats were divided into control group (uninfected) and Experimental Group (Infected). Half of the animals had their wounds irradiated. RESULTS: in vitro: there was no statistically significant variation between the experimental groups as for the source plates and the derived ones (p>0.05). In vivo: there was a significant increase in the deposition of type I and III collagen in the wounds of the infected and irradiated animals when assessed on the fourth day of the experiment (p=0.034). CONCLUSION: low-intensity Laser Therapy applied with a wavelength of 904nm and dose 3J/cm2 did not alter the in vitro growth of S. aureus in experimental groups; in vivo, however, it showed significant increase in the deposition of type I and III collagen in the wound of infected and irradiated animals on the fourth day of the experiment. |
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Revista do Colégio Brasileiro de Cirurgiões |
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Effects of low intensity laser in in vitro bacterial culture and in vivo infected woundsInfectionLaser therapyLaser therapy, low-levelWound healingIn vitro OBJECTIVE: to compare the effects of low intensity laser therapy on in vitro bacterial growth and in vivo in infected wounds, and to analyze the effectiveness of the AsGa Laser technology in in vivo wound infections. METHODS: in vitro: Staphylococcus aureus were incubated on blood agar plates, half of them being irradiated with 904 nm wavelength laser and dose of 3J/cm2 daily for seven days. In vivo: 32 male Wistar rats were divided into control group (uninfected) and Experimental Group (Infected). Half of the animals had their wounds irradiated. RESULTS: in vitro: there was no statistically significant variation between the experimental groups as for the source plates and the derived ones (p>0.05). In vivo: there was a significant increase in the deposition of type I and III collagen in the wounds of the infected and irradiated animals when assessed on the fourth day of the experiment (p=0.034). CONCLUSION: low-intensity Laser Therapy applied with a wavelength of 904nm and dose 3J/cm2 did not alter the in vitro growth of S. aureus in experimental groups; in vivo, however, it showed significant increase in the deposition of type I and III collagen in the wound of infected and irradiated animals on the fourth day of the experiment. Colégio Brasileiro de Cirurgiões2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912014000100049Revista do Colégio Brasileiro de Cirurgiões v.41 n.1 2014reponame:Revista do Colégio Brasileiro de Cirurgiõesinstname:Colégio Brasileiro de Cirurgiões (CBC)instacron:CBC10.1590/S0100-69912014000100010info:eu-repo/semantics/openAccessPereira,Pâmela RosaDe Paula,Josue BruginskiCielinski,JuliettePilonetto,MarceloVon Bahten,Luiz Carloseng2014-04-22T00:00:00Zoai:scielo:S0100-69912014000100049Revistahttp://www.scielo.br/rcbcONGhttps://old.scielo.br/oai/scielo-oai.php||revistacbc@cbc.org.br1809-45460100-6991opendoar:2014-04-22T00:00Revista do Colégio Brasileiro de Cirurgiões - Colégio Brasileiro de Cirurgiões (CBC)false |
dc.title.none.fl_str_mv |
Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds |
title |
Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds |
spellingShingle |
Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds Pereira,Pâmela Rosa Infection Laser therapy Laser therapy, low-level Wound healing In vitro |
title_short |
Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds |
title_full |
Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds |
title_fullStr |
Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds |
title_full_unstemmed |
Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds |
title_sort |
Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds |
author |
Pereira,Pâmela Rosa |
author_facet |
Pereira,Pâmela Rosa De Paula,Josue Bruginski Cielinski,Juliette Pilonetto,Marcelo Von Bahten,Luiz Carlos |
author_role |
author |
author2 |
De Paula,Josue Bruginski Cielinski,Juliette Pilonetto,Marcelo Von Bahten,Luiz Carlos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pereira,Pâmela Rosa De Paula,Josue Bruginski Cielinski,Juliette Pilonetto,Marcelo Von Bahten,Luiz Carlos |
dc.subject.por.fl_str_mv |
Infection Laser therapy Laser therapy, low-level Wound healing In vitro |
topic |
Infection Laser therapy Laser therapy, low-level Wound healing In vitro |
description |
OBJECTIVE: to compare the effects of low intensity laser therapy on in vitro bacterial growth and in vivo in infected wounds, and to analyze the effectiveness of the AsGa Laser technology in in vivo wound infections. METHODS: in vitro: Staphylococcus aureus were incubated on blood agar plates, half of them being irradiated with 904 nm wavelength laser and dose of 3J/cm2 daily for seven days. In vivo: 32 male Wistar rats were divided into control group (uninfected) and Experimental Group (Infected). Half of the animals had their wounds irradiated. RESULTS: in vitro: there was no statistically significant variation between the experimental groups as for the source plates and the derived ones (p>0.05). In vivo: there was a significant increase in the deposition of type I and III collagen in the wounds of the infected and irradiated animals when assessed on the fourth day of the experiment (p=0.034). CONCLUSION: low-intensity Laser Therapy applied with a wavelength of 904nm and dose 3J/cm2 did not alter the in vitro growth of S. aureus in experimental groups; in vivo, however, it showed significant increase in the deposition of type I and III collagen in the wound of infected and irradiated animals on the fourth day of the experiment. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912014000100049 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912014000100049 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-69912014000100010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Cirurgiões |
publisher.none.fl_str_mv |
Colégio Brasileiro de Cirurgiões |
dc.source.none.fl_str_mv |
Revista do Colégio Brasileiro de Cirurgiões v.41 n.1 2014 reponame:Revista do Colégio Brasileiro de Cirurgiões instname:Colégio Brasileiro de Cirurgiões (CBC) instacron:CBC |
instname_str |
Colégio Brasileiro de Cirurgiões (CBC) |
instacron_str |
CBC |
institution |
CBC |
reponame_str |
Revista do Colégio Brasileiro de Cirurgiões |
collection |
Revista do Colégio Brasileiro de Cirurgiões |
repository.name.fl_str_mv |
Revista do Colégio Brasileiro de Cirurgiões - Colégio Brasileiro de Cirurgiões (CBC) |
repository.mail.fl_str_mv |
||revistacbc@cbc.org.br |
_version_ |
1754209212652584960 |