Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000100008 |
Resumo: | Ehrlichiosis is a tick-borne disease that affects both humans and animals. The few existing reports on ehrlichiosis in Brazilian cats have been based on observation of morulae in leukocytes and, more recently, on molecular detection of Ehrlichia sp. In this study, we assessed occurrences of Ehrlichia sp. in the blood of 200 domestic cats in São Luís, Maranhão. Of the 200 animals tested, 11 (5.5%) were seropositive for Ehrlichia sp. and two (1%) were positive for Ehrlichia sp. in PCR. We also performed DNA sequence alignment to establish the identity of the parasite species infecting these animals, using the gene 16S rRNA. One cat presented infection with Ehrlichia sp. with 98% identity with E. canis, and another cat infected with Ehrlichia sp. showed 97% identity with E. chaffeensis. This is the first study on molecular detection of Ehrlichia sp. among domestic cats in São Luís, Maranhão. |
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Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, BrazilCatsEhrlichia sp.serologyPCRBrazilEhrlichiosis is a tick-borne disease that affects both humans and animals. The few existing reports on ehrlichiosis in Brazilian cats have been based on observation of morulae in leukocytes and, more recently, on molecular detection of Ehrlichia sp. In this study, we assessed occurrences of Ehrlichia sp. in the blood of 200 domestic cats in São Luís, Maranhão. Of the 200 animals tested, 11 (5.5%) were seropositive for Ehrlichia sp. and two (1%) were positive for Ehrlichia sp. in PCR. We also performed DNA sequence alignment to establish the identity of the parasite species infecting these animals, using the gene 16S rRNA. One cat presented infection with Ehrlichia sp. with 98% identity with E. canis, and another cat infected with Ehrlichia sp. showed 97% identity with E. chaffeensis. This is the first study on molecular detection of Ehrlichia sp. among domestic cats in São Luís, Maranhão.Colégio Brasileiro de Parasitologia Veterinária2012-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000100008Revista Brasileira de Parasitologia Veterinária v.21 n.1 2012reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612012000100008info:eu-repo/semantics/openAccessBraga,Maria do Socorro Costa de OliveiraAndré,Marcos RogérioFreschi,Carla RobertaTeixeira,Márcia Cristina AlvesMachado,Rosangela Zacariaseng2012-04-23T00:00:00Zoai:scielo:S1984-29612012000100008Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2012-04-23T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, Brazil |
title |
Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, Brazil |
spellingShingle |
Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, Brazil Braga,Maria do Socorro Costa de Oliveira Cats Ehrlichia sp. serology PCR Brazil |
title_short |
Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, Brazil |
title_full |
Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, Brazil |
title_fullStr |
Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, Brazil |
title_full_unstemmed |
Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, Brazil |
title_sort |
Molecular and serological detection of Ehrlichia spp. in cats on São Luís Island, Maranhão, Brazil |
author |
Braga,Maria do Socorro Costa de Oliveira |
author_facet |
Braga,Maria do Socorro Costa de Oliveira André,Marcos Rogério Freschi,Carla Roberta Teixeira,Márcia Cristina Alves Machado,Rosangela Zacarias |
author_role |
author |
author2 |
André,Marcos Rogério Freschi,Carla Roberta Teixeira,Márcia Cristina Alves Machado,Rosangela Zacarias |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Braga,Maria do Socorro Costa de Oliveira André,Marcos Rogério Freschi,Carla Roberta Teixeira,Márcia Cristina Alves Machado,Rosangela Zacarias |
dc.subject.por.fl_str_mv |
Cats Ehrlichia sp. serology PCR Brazil |
topic |
Cats Ehrlichia sp. serology PCR Brazil |
description |
Ehrlichiosis is a tick-borne disease that affects both humans and animals. The few existing reports on ehrlichiosis in Brazilian cats have been based on observation of morulae in leukocytes and, more recently, on molecular detection of Ehrlichia sp. In this study, we assessed occurrences of Ehrlichia sp. in the blood of 200 domestic cats in São Luís, Maranhão. Of the 200 animals tested, 11 (5.5%) were seropositive for Ehrlichia sp. and two (1%) were positive for Ehrlichia sp. in PCR. We also performed DNA sequence alignment to establish the identity of the parasite species infecting these animals, using the gene 16S rRNA. One cat presented infection with Ehrlichia sp. with 98% identity with E. canis, and another cat infected with Ehrlichia sp. showed 97% identity with E. chaffeensis. This is the first study on molecular detection of Ehrlichia sp. among domestic cats in São Luís, Maranhão. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000100008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000100008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612012000100008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.21 n.1 2012 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
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Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
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CBPV |
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CBPV |
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Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208914067423232 |