Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000200187 |
Resumo: | Abstract Giardia duodenalis is divided into eight assemblages (named A to H). Isolates of assemblage A are divided into four sub-assemblages (AI, AII, AIII and AIV). While isolates of sub-assemblage AII are almost exclusively detected in human hosts, isolates of assemblage B are encountered in a multitude of animal hosts and humans. Here, we isolated single cysts of G. duodenalis from a human stool sample and found that one of them had overlaps of assemblage AII and B alleles and an unexpectedly high number of variants of the beta-giardin (Bg) and GLORF-C4 (OrfC4) alleles. In addition, one of the Bg alleles of that cyst had a fragment of sub-assemblage AII interspersed with fragments of assemblage B, thus indicating that this allele may be a recombinant between sequences A and B. Our results are unprecedented and put a check on the statement that different assemblages of G. duodenalis represent species with different host specificities. |
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Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalisGiardia duodenalismicromanipulationheterozygositygenetic recombinationcystsAbstract Giardia duodenalis is divided into eight assemblages (named A to H). Isolates of assemblage A are divided into four sub-assemblages (AI, AII, AIII and AIV). While isolates of sub-assemblage AII are almost exclusively detected in human hosts, isolates of assemblage B are encountered in a multitude of animal hosts and humans. Here, we isolated single cysts of G. duodenalis from a human stool sample and found that one of them had overlaps of assemblage AII and B alleles and an unexpectedly high number of variants of the beta-giardin (Bg) and GLORF-C4 (OrfC4) alleles. In addition, one of the Bg alleles of that cyst had a fragment of sub-assemblage AII interspersed with fragments of assemblage B, thus indicating that this allele may be a recombinant between sequences A and B. Our results are unprecedented and put a check on the statement that different assemblages of G. duodenalis represent species with different host specificities.Colégio Brasileiro de Parasitologia Veterinária2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000200187Revista Brasileira de Parasitologia Veterinária v.25 n.2 2016reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612016031info:eu-repo/semantics/openAccessAguiar,Juliana MartinsSilva,Sheila OliveiraSantos,Valdir Azevedo dosTaniwaki,Sueli AkemiOliveira,Tricia Maria Ferreira de SousaFerreira,Helena LageKeid,Lara BorgesGregori,FábioSoares,Rodrigo Martinseng2016-06-15T00:00:00Zoai:scielo:S1984-29612016000200187Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2016-06-15T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis |
title |
Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis |
spellingShingle |
Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis Aguiar,Juliana Martins Giardia duodenalis micromanipulation heterozygosity genetic recombination cysts |
title_short |
Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis |
title_full |
Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis |
title_fullStr |
Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis |
title_full_unstemmed |
Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis |
title_sort |
Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis |
author |
Aguiar,Juliana Martins |
author_facet |
Aguiar,Juliana Martins Silva,Sheila Oliveira Santos,Valdir Azevedo dos Taniwaki,Sueli Akemi Oliveira,Tricia Maria Ferreira de Sousa Ferreira,Helena Lage Keid,Lara Borges Gregori,Fábio Soares,Rodrigo Martins |
author_role |
author |
author2 |
Silva,Sheila Oliveira Santos,Valdir Azevedo dos Taniwaki,Sueli Akemi Oliveira,Tricia Maria Ferreira de Sousa Ferreira,Helena Lage Keid,Lara Borges Gregori,Fábio Soares,Rodrigo Martins |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Aguiar,Juliana Martins Silva,Sheila Oliveira Santos,Valdir Azevedo dos Taniwaki,Sueli Akemi Oliveira,Tricia Maria Ferreira de Sousa Ferreira,Helena Lage Keid,Lara Borges Gregori,Fábio Soares,Rodrigo Martins |
dc.subject.por.fl_str_mv |
Giardia duodenalis micromanipulation heterozygosity genetic recombination cysts |
topic |
Giardia duodenalis micromanipulation heterozygosity genetic recombination cysts |
description |
Abstract Giardia duodenalis is divided into eight assemblages (named A to H). Isolates of assemblage A are divided into four sub-assemblages (AI, AII, AIII and AIV). While isolates of sub-assemblage AII are almost exclusively detected in human hosts, isolates of assemblage B are encountered in a multitude of animal hosts and humans. Here, we isolated single cysts of G. duodenalis from a human stool sample and found that one of them had overlaps of assemblage AII and B alleles and an unexpectedly high number of variants of the beta-giardin (Bg) and GLORF-C4 (OrfC4) alleles. In addition, one of the Bg alleles of that cyst had a fragment of sub-assemblage AII interspersed with fragments of assemblage B, thus indicating that this allele may be a recombinant between sequences A and B. Our results are unprecedented and put a check on the statement that different assemblages of G. duodenalis represent species with different host specificities. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000200187 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000200187 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612016031 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.25 n.2 2016 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208916246364160 |