Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil
Main Author: | |
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Publication Date: | 2016 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Revista Brasileira de Parasitologia Veterinária (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400418 |
Summary: | Abstract The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT) was performed on serum samples and positive samples were subjected to western blot (WB) analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6%) humans, 26/83 (31.3%) dogs, and 7/18 (38.9%) horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34) horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis. |
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Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern BrazilImmunofluorescence antibody test (IFAT)Lyme diseaseserologytick-borne diseasewestern blotAbstract The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT) was performed on serum samples and positive samples were subjected to western blot (WB) analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6%) humans, 26/83 (31.3%) dogs, and 7/18 (38.9%) horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34) horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis.Colégio Brasileiro de Parasitologia Veterinária2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400418Revista Brasileira de Parasitologia Veterinária v.25 n.4 2016reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612016085info:eu-repo/semantics/openAccessNascimento,Denise Amaral GomesVieira,Rafael Felipe da CostaVieira,Thállitha Samih Wischral JaymeToledo,Roberta dos SantosTamekuni,KatiaSantos,Nelson Jessé Rodrigues dosGonçalves,Daniela DibbVieira,Maria LuísaBiondo,Alexander WelkerVidotto,Odiloneng2017-11-06T00:00:00Zoai:scielo:S1984-29612016000400418Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2017-11-06T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
title |
Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
spellingShingle |
Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil Nascimento,Denise Amaral Gomes Immunofluorescence antibody test (IFAT) Lyme disease serology tick-borne disease western blot |
title_short |
Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
title_full |
Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
title_fullStr |
Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
title_full_unstemmed |
Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
title_sort |
Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil |
author |
Nascimento,Denise Amaral Gomes |
author_facet |
Nascimento,Denise Amaral Gomes Vieira,Rafael Felipe da Costa Vieira,Thállitha Samih Wischral Jayme Toledo,Roberta dos Santos Tamekuni,Katia Santos,Nelson Jessé Rodrigues dos Gonçalves,Daniela Dibb Vieira,Maria Luísa Biondo,Alexander Welker Vidotto,Odilon |
author_role |
author |
author2 |
Vieira,Rafael Felipe da Costa Vieira,Thállitha Samih Wischral Jayme Toledo,Roberta dos Santos Tamekuni,Katia Santos,Nelson Jessé Rodrigues dos Gonçalves,Daniela Dibb Vieira,Maria Luísa Biondo,Alexander Welker Vidotto,Odilon |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento,Denise Amaral Gomes Vieira,Rafael Felipe da Costa Vieira,Thállitha Samih Wischral Jayme Toledo,Roberta dos Santos Tamekuni,Katia Santos,Nelson Jessé Rodrigues dos Gonçalves,Daniela Dibb Vieira,Maria Luísa Biondo,Alexander Welker Vidotto,Odilon |
dc.subject.por.fl_str_mv |
Immunofluorescence antibody test (IFAT) Lyme disease serology tick-borne disease western blot |
topic |
Immunofluorescence antibody test (IFAT) Lyme disease serology tick-borne disease western blot |
description |
Abstract The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT) was performed on serum samples and positive samples were subjected to western blot (WB) analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6%) humans, 26/83 (31.3%) dogs, and 7/18 (38.9%) horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34) horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400418 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400418 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-29612016085 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.25 n.4 2016 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
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Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
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CBPV |
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CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
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Revista Brasileira de Parasitologia Veterinária (Online) |
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Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
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||zacariascbpv@fcav.unesp.br |
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1754208916586102784 |