Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000300339 |
Resumo: | This study aimed to analyze ovary histology and quantify total protein in the hemolymph of Rhipicephalus (Boophilus)microplus females treated with hexane extracts from green fruits of Melia azedarach. Eight engorged females were immersed in the extract at 0.25% concentration, and eight in water containing 5% acetone (control). The females were dissected 72 hours after treatment, and the ovaries were weighed and subjected to standard histological techniques. The total protein concentration was measured in the hemolymph of 200 females, of which 100 were treated as described above and 100 served as a control. In the treated group, ovary weight reduction and predominance of immature oocytes were observed. In addition, there were decreases in the diameters of the cytoplasm and germ vesicle of the oocytes in the treated group, compared with the controls. The protein concentration in the hemolymph was higher in the treated group than in the controls. The morphological changes observed in the treated ovaries included: presence of vacuolization; alteration of oocyte morphology, which changed from rounded to elongated; deformation of the chorion; and disorganization of the yolk granules. These results demonstrate the action ofM. azedarach fruit extracts on R.(B.) microplus oogenesis. |
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Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarachRhipicephalus (Boophilus)microplusoocytesovaryhistologyhemolymph proteinsMelia azedarachThis study aimed to analyze ovary histology and quantify total protein in the hemolymph of Rhipicephalus (Boophilus)microplus females treated with hexane extracts from green fruits of Melia azedarach. Eight engorged females were immersed in the extract at 0.25% concentration, and eight in water containing 5% acetone (control). The females were dissected 72 hours after treatment, and the ovaries were weighed and subjected to standard histological techniques. The total protein concentration was measured in the hemolymph of 200 females, of which 100 were treated as described above and 100 served as a control. In the treated group, ovary weight reduction and predominance of immature oocytes were observed. In addition, there were decreases in the diameters of the cytoplasm and germ vesicle of the oocytes in the treated group, compared with the controls. The protein concentration in the hemolymph was higher in the treated group than in the controls. The morphological changes observed in the treated ovaries included: presence of vacuolization; alteration of oocyte morphology, which changed from rounded to elongated; deformation of the chorion; and disorganization of the yolk granules. These results demonstrate the action ofM. azedarach fruit extracts on R.(B.) microplus oogenesis.Colégio Brasileiro de Parasitologia Veterinária2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000300339Revista Brasileira de Parasitologia Veterinária v.22 n.3 2013reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612013000300004info:eu-repo/semantics/openAccessSousa,Lorena Alessandra Dias deRocha,Thiago LopesSabóia-Morais,Simone Maria TeixeiraBorges,Lígia Miranda Ferreiraeng2015-07-30T00:00:00Zoai:scielo:S1984-29612013000300339Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2015-07-30T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach |
title |
Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach |
spellingShingle |
Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach Sousa,Lorena Alessandra Dias de Rhipicephalus (Boophilus)microplus oocytes ovary histology hemolymph proteins Melia azedarach |
title_short |
Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach |
title_full |
Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach |
title_fullStr |
Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach |
title_full_unstemmed |
Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach |
title_sort |
Ovary histology and quantification of hemolymph proteins of Rhipicephalus (Boophilus)microplus treated with Melia azedarach |
author |
Sousa,Lorena Alessandra Dias de |
author_facet |
Sousa,Lorena Alessandra Dias de Rocha,Thiago Lopes Sabóia-Morais,Simone Maria Teixeira Borges,Lígia Miranda Ferreira |
author_role |
author |
author2 |
Rocha,Thiago Lopes Sabóia-Morais,Simone Maria Teixeira Borges,Lígia Miranda Ferreira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Sousa,Lorena Alessandra Dias de Rocha,Thiago Lopes Sabóia-Morais,Simone Maria Teixeira Borges,Lígia Miranda Ferreira |
dc.subject.por.fl_str_mv |
Rhipicephalus (Boophilus)microplus oocytes ovary histology hemolymph proteins Melia azedarach |
topic |
Rhipicephalus (Boophilus)microplus oocytes ovary histology hemolymph proteins Melia azedarach |
description |
This study aimed to analyze ovary histology and quantify total protein in the hemolymph of Rhipicephalus (Boophilus)microplus females treated with hexane extracts from green fruits of Melia azedarach. Eight engorged females were immersed in the extract at 0.25% concentration, and eight in water containing 5% acetone (control). The females were dissected 72 hours after treatment, and the ovaries were weighed and subjected to standard histological techniques. The total protein concentration was measured in the hemolymph of 200 females, of which 100 were treated as described above and 100 served as a control. In the treated group, ovary weight reduction and predominance of immature oocytes were observed. In addition, there were decreases in the diameters of the cytoplasm and germ vesicle of the oocytes in the treated group, compared with the controls. The protein concentration in the hemolymph was higher in the treated group than in the controls. The morphological changes observed in the treated ovaries included: presence of vacuolization; alteration of oocyte morphology, which changed from rounded to elongated; deformation of the chorion; and disorganization of the yolk granules. These results demonstrate the action ofM. azedarach fruit extracts on R.(B.) microplus oogenesis. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000300339 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000300339 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612013000300004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.22 n.3 2013 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208914822397952 |