Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400383 |
Resumo: | Abstract The anthelminthic activity of the essential oil (EO) of Piper aduncum L. was tested in vitro on eggs and larvae of resistant (Embrapa2010) and susceptible (McMaster) isolates of Haemonchus contortus. The EO was obtained by steam distillation and its components identified by chromatography. EO concentrations of 12.5 to 0.02 mg/mL were used in the egg hatch test (EHT) and concentrations of 3.12 to 0.01 mg/mL in the larval development test (LDT). Inhibition concentrations (IC) were determined by the SAS Probit procedure, and significant differences assessed by ANOVA followed by Tukey’s test. In the EHT, the IC50 for the susceptible isolate was 5.72 mg/mL. In the LDT, the IC50 and IC90 were, respectively, 0.10 mg/mL and 0.34 mg/mL for the susceptible isolate, and 0.22 mg/mL and 0.51 mg/mL for the resistant isolate. The EO (dillapiole 76.2%) was highly efficacious on phase L1. Due to the higher ICs obtained for the resistant isolate, it was raised the hypothesis that dillapiole may have a mechanism of action that resembles those of other anthelmintic compounds. We further review and discuss studies, especially those conducted in Brazil, that quantified the major constituents of P. aduncum-derived EO. |
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Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compoundsIsolatesHaemonchus contortuscross resistancedillapiolesynergyAbstract The anthelminthic activity of the essential oil (EO) of Piper aduncum L. was tested in vitro on eggs and larvae of resistant (Embrapa2010) and susceptible (McMaster) isolates of Haemonchus contortus. The EO was obtained by steam distillation and its components identified by chromatography. EO concentrations of 12.5 to 0.02 mg/mL were used in the egg hatch test (EHT) and concentrations of 3.12 to 0.01 mg/mL in the larval development test (LDT). Inhibition concentrations (IC) were determined by the SAS Probit procedure, and significant differences assessed by ANOVA followed by Tukey’s test. In the EHT, the IC50 for the susceptible isolate was 5.72 mg/mL. In the LDT, the IC50 and IC90 were, respectively, 0.10 mg/mL and 0.34 mg/mL for the susceptible isolate, and 0.22 mg/mL and 0.51 mg/mL for the resistant isolate. The EO (dillapiole 76.2%) was highly efficacious on phase L1. Due to the higher ICs obtained for the resistant isolate, it was raised the hypothesis that dillapiole may have a mechanism of action that resembles those of other anthelmintic compounds. We further review and discuss studies, especially those conducted in Brazil, that quantified the major constituents of P. aduncum-derived EO.Colégio Brasileiro de Parasitologia Veterinária2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400383Revista Brasileira de Parasitologia Veterinária v.25 n.4 2016reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612016073info:eu-repo/semantics/openAccessGaínza,Yousmel AlemánFantatto,Rafaela ReginaChaves,Francisco Celio MaiaBizzo,Humberto RibeiroEsteves,Sérgio NovitaChagas,Ana Carolina de Souzaeng2017-11-06T00:00:00Zoai:scielo:S1984-29612016000400383Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2017-11-06T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds |
title |
Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds |
spellingShingle |
Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds Gaínza,Yousmel Alemán Isolates Haemonchus contortus cross resistance dillapiole synergy |
title_short |
Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds |
title_full |
Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds |
title_fullStr |
Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds |
title_full_unstemmed |
Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds |
title_sort |
Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds |
author |
Gaínza,Yousmel Alemán |
author_facet |
Gaínza,Yousmel Alemán Fantatto,Rafaela Regina Chaves,Francisco Celio Maia Bizzo,Humberto Ribeiro Esteves,Sérgio Novita Chagas,Ana Carolina de Souza |
author_role |
author |
author2 |
Fantatto,Rafaela Regina Chaves,Francisco Celio Maia Bizzo,Humberto Ribeiro Esteves,Sérgio Novita Chagas,Ana Carolina de Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gaínza,Yousmel Alemán Fantatto,Rafaela Regina Chaves,Francisco Celio Maia Bizzo,Humberto Ribeiro Esteves,Sérgio Novita Chagas,Ana Carolina de Souza |
dc.subject.por.fl_str_mv |
Isolates Haemonchus contortus cross resistance dillapiole synergy |
topic |
Isolates Haemonchus contortus cross resistance dillapiole synergy |
description |
Abstract The anthelminthic activity of the essential oil (EO) of Piper aduncum L. was tested in vitro on eggs and larvae of resistant (Embrapa2010) and susceptible (McMaster) isolates of Haemonchus contortus. The EO was obtained by steam distillation and its components identified by chromatography. EO concentrations of 12.5 to 0.02 mg/mL were used in the egg hatch test (EHT) and concentrations of 3.12 to 0.01 mg/mL in the larval development test (LDT). Inhibition concentrations (IC) were determined by the SAS Probit procedure, and significant differences assessed by ANOVA followed by Tukey’s test. In the EHT, the IC50 for the susceptible isolate was 5.72 mg/mL. In the LDT, the IC50 and IC90 were, respectively, 0.10 mg/mL and 0.34 mg/mL for the susceptible isolate, and 0.22 mg/mL and 0.51 mg/mL for the resistant isolate. The EO (dillapiole 76.2%) was highly efficacious on phase L1. Due to the higher ICs obtained for the resistant isolate, it was raised the hypothesis that dillapiole may have a mechanism of action that resembles those of other anthelmintic compounds. We further review and discuss studies, especially those conducted in Brazil, that quantified the major constituents of P. aduncum-derived EO. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400383 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400383 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-29612016073 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.25 n.4 2016 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208916569325568 |