Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturais
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-29612016073 http://hdl.handle.net/11449/169255 |
Resumo: | The anthelminthic activity of the essential oil (EO) of Piper aduncum L. was tested in vitro on eggs and larvae of resistant (Embrapa2010) and susceptible (McMaster) isolates of Haemonchus contortus. The EO was obtained by steam distillation and its components identified by chromatography. EO concentrations of 12.5 to 0.02 mg/mL were used in the egg hatch test (EHT) and concentrations of 3.12 to 0.01 mg/mL in the larval development test (LDT). Inhibition concentrations (IC) were determined by the SAS Probit procedure, and significant differences assessed by ANOVA followed by Tukey’s test. In the EHT, the IC50 for the susceptible isolate was 5.72 mg/mL. In the LDT, the IC50 and IC90 were, respectively, 0.10 mg/mL and 0.34 mg/mL for the susceptible isolate, and 0.22 mg/mL and 0.51 mg/mL for the resistant isolate. The EO (dillapiole 76.2%) was highly efficacious on phase L1. Due to the higher ICs obtained for the resistant isolate, it was raised the hypothesis that dillapiole may have a mechanism of action that resembles those of other anthelmintic compounds. We further review and discuss studies, especially those conducted in Brazil, that quantified the major constituents of P. aduncum-derived EO. |
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Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturaisPiper aduncum against Haemonchus contortus isolates: Cross resistance and the research of natural bioactive compoundsCross resistanceDillapioleHaemonchus contortusIsolatesSynergyThe anthelminthic activity of the essential oil (EO) of Piper aduncum L. was tested in vitro on eggs and larvae of resistant (Embrapa2010) and susceptible (McMaster) isolates of Haemonchus contortus. The EO was obtained by steam distillation and its components identified by chromatography. EO concentrations of 12.5 to 0.02 mg/mL were used in the egg hatch test (EHT) and concentrations of 3.12 to 0.01 mg/mL in the larval development test (LDT). Inhibition concentrations (IC) were determined by the SAS Probit procedure, and significant differences assessed by ANOVA followed by Tukey’s test. In the EHT, the IC50 for the susceptible isolate was 5.72 mg/mL. In the LDT, the IC50 and IC90 were, respectively, 0.10 mg/mL and 0.34 mg/mL for the susceptible isolate, and 0.22 mg/mL and 0.51 mg/mL for the resistant isolate. The EO (dillapiole 76.2%) was highly efficacious on phase L1. Due to the higher ICs obtained for the resistant isolate, it was raised the hypothesis that dillapiole may have a mechanism of action that resembles those of other anthelmintic compounds. We further review and discuss studies, especially those conducted in Brazil, that quantified the major constituents of P. aduncum-derived EO.Fundação de Amparo à Pesquisa do Estado do AmazonasCentro Nacional de Sanidad Agropecuaria-CENSA San José de las LajasUniversidade Estadual Paulista “Júlio de Mesquita Filho”-UNESPEmbrapa Amazônia OcidentalEmbrapa Agroindústria de AlimentosEmbrapa Pecuária SudesteUniversidade Estadual Paulista “Júlio de Mesquita Filho”-UNESPFundação de Amparo à Pesquisa do Estado do Amazonas: 062.02821/2014San José de las LajasUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Gaínza, Yousmel AlemánFantatto, Rafaela Regina [UNESP]Chaves, Francisco Celio MaiaBizzo, Humberto RibeiroEsteves, Sérgio NovitaChagas, Ana Carolina de Souza2018-12-11T16:45:06Z2018-12-11T16:45:06Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article383-393application/pdfhttp://dx.doi.org/10.1590/S1984-29612016073Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 383-393, 2016.0103-846Xhttp://hdl.handle.net/11449/16925510.1590/S1984-29612016073S1984-296120160004003832-s2.0-85006802723S1984-29612016000400383.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2023-11-11T06:08:50Zoai:repositorio.unesp.br:11449/169255Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-11T06:08:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturais Piper aduncum against Haemonchus contortus isolates: Cross resistance and the research of natural bioactive compounds |
title |
Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturais |
spellingShingle |
Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturais Gaínza, Yousmel Alemán Cross resistance Dillapiole Haemonchus contortus Isolates Synergy |
title_short |
Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturais |
title_full |
Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturais |
title_fullStr |
Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturais |
title_full_unstemmed |
Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturais |
title_sort |
Piper aduncum contra isolados de Haemonchus contortus: Resistência cruzada e a pesquisa de bioativos naturais |
author |
Gaínza, Yousmel Alemán |
author_facet |
Gaínza, Yousmel Alemán Fantatto, Rafaela Regina [UNESP] Chaves, Francisco Celio Maia Bizzo, Humberto Ribeiro Esteves, Sérgio Novita Chagas, Ana Carolina de Souza |
author_role |
author |
author2 |
Fantatto, Rafaela Regina [UNESP] Chaves, Francisco Celio Maia Bizzo, Humberto Ribeiro Esteves, Sérgio Novita Chagas, Ana Carolina de Souza |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
San José de las Lajas Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Gaínza, Yousmel Alemán Fantatto, Rafaela Regina [UNESP] Chaves, Francisco Celio Maia Bizzo, Humberto Ribeiro Esteves, Sérgio Novita Chagas, Ana Carolina de Souza |
dc.subject.por.fl_str_mv |
Cross resistance Dillapiole Haemonchus contortus Isolates Synergy |
topic |
Cross resistance Dillapiole Haemonchus contortus Isolates Synergy |
description |
The anthelminthic activity of the essential oil (EO) of Piper aduncum L. was tested in vitro on eggs and larvae of resistant (Embrapa2010) and susceptible (McMaster) isolates of Haemonchus contortus. The EO was obtained by steam distillation and its components identified by chromatography. EO concentrations of 12.5 to 0.02 mg/mL were used in the egg hatch test (EHT) and concentrations of 3.12 to 0.01 mg/mL in the larval development test (LDT). Inhibition concentrations (IC) were determined by the SAS Probit procedure, and significant differences assessed by ANOVA followed by Tukey’s test. In the EHT, the IC50 for the susceptible isolate was 5.72 mg/mL. In the LDT, the IC50 and IC90 were, respectively, 0.10 mg/mL and 0.34 mg/mL for the susceptible isolate, and 0.22 mg/mL and 0.51 mg/mL for the resistant isolate. The EO (dillapiole 76.2%) was highly efficacious on phase L1. Due to the higher ICs obtained for the resistant isolate, it was raised the hypothesis that dillapiole may have a mechanism of action that resembles those of other anthelmintic compounds. We further review and discuss studies, especially those conducted in Brazil, that quantified the major constituents of P. aduncum-derived EO. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T16:45:06Z 2018-12-11T16:45:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612016073 Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 383-393, 2016. 0103-846X http://hdl.handle.net/11449/169255 10.1590/S1984-29612016073 S1984-29612016000400383 2-s2.0-85006802723 S1984-29612016000400383.pdf |
url |
http://dx.doi.org/10.1590/S1984-29612016073 http://hdl.handle.net/11449/169255 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 4, p. 383-393, 2016. 0103-846X 10.1590/S1984-29612016073 S1984-29612016000400383 2-s2.0-85006802723 S1984-29612016000400383.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
383-393 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792961789684613120 |