Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood cultures
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Health & Biological Sciences |
Texto Completo: | https://periodicos.unichristus.edu.br/jhbs/article/view/27 |
Resumo: | Background: In Brazil, listeriosis is not a notifiable disease; thus, the incidence of Brazilian cases remains unknown. Listeria monocytogenes is not always included in automated systems, and its detection depends on the high skill level of microbiology laboratory professionals. This paper describes the characteristics of L. monocytogenes isolates fortuitously obtained from an endocarditis case in Recife, PE, Brazil. Methods: Six bacterial isolates obtained from six blood cultures from a 28-year-old male bearing a prosthetic mitral heart valve were analyzed by PCR using primers specific of L. monocytogenes to confirm a presumptive identification, determine the serotype and presence of the virulence genes (inlA, inlB, inlC, inlJ, hly, plcA, actA, prfA) in an attempt to determine the Listeria genotype by PCR-ribotyping. Results: The samples were identified as L. monocytogenes 4b. All investigated virulence genes were amplified by PCR, and the identity of the amplified segments was confirmed by sequencing. A deletion of 105 base pairs was detected in the actA gene. All of the samples generated the same PCR-ribotype pattern, clustered into a single ribotype, and were considered a single strain. Conclusion: L. monocytogenes infection should be considered in endocarditis differential diagnoses, especially among high-risk groups, due to its high pathogenicity and the environmental ubiquity. |
id |
CHRISTUS-1_32b81b81a1a6f3246d28b06587b739af |
---|---|
oai_identifier_str |
oai:ojs.unichristus.emnuvens.com.br:article/27 |
network_acronym_str |
CHRISTUS-1 |
network_name_str |
Journal of Health & Biological Sciences |
repository_id_str |
|
spelling |
Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood culturesSaúde; Ciências Biológicas; Microbiologia; Genética de Micro-organismosListeria; Listeriose; Listeria monocytogenes; Endocardite; VirulênciaQW 25.5.B2Background: In Brazil, listeriosis is not a notifiable disease; thus, the incidence of Brazilian cases remains unknown. Listeria monocytogenes is not always included in automated systems, and its detection depends on the high skill level of microbiology laboratory professionals. This paper describes the characteristics of L. monocytogenes isolates fortuitously obtained from an endocarditis case in Recife, PE, Brazil. Methods: Six bacterial isolates obtained from six blood cultures from a 28-year-old male bearing a prosthetic mitral heart valve were analyzed by PCR using primers specific of L. monocytogenes to confirm a presumptive identification, determine the serotype and presence of the virulence genes (inlA, inlB, inlC, inlJ, hly, plcA, actA, prfA) in an attempt to determine the Listeria genotype by PCR-ribotyping. Results: The samples were identified as L. monocytogenes 4b. All investigated virulence genes were amplified by PCR, and the identity of the amplified segments was confirmed by sequencing. A deletion of 105 base pairs was detected in the actA gene. All of the samples generated the same PCR-ribotype pattern, clustered into a single ribotype, and were considered a single strain. Conclusion: L. monocytogenes infection should be considered in endocarditis differential diagnoses, especially among high-risk groups, due to its high pathogenicity and the environmental ubiquity.Instituto para o Desenvolvimento da EducacaoLeal, Nilma CintraCosta, Ana Paula Rocha daVilela, Marinalda AnselmoMendes-Marques, Carina LucenaAlmeida, Alzira Maria Paiva de2013-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por ParesPeer ReviewRevisado por paresapplication/pdfhttps://periodicos.unichristus.edu.br/jhbs/article/view/2710.12662/2317-3076jhbs.v1i3.27.p116.2013Journal of Health & Biological Sciences; v. 1, n. 3 (2013): Journal of Health & Biological Sciences; 116Journal of Health & Biological Sciences; v. 1, n. 3 (2013): Journal of Health & Biological Sciences; 116Journal of Health and Biological Sciences; v. 1, n. 3 (2013): Journal of Health & Biological Sciences; 1162317-30762317-308410.12662/2317-3076jhbs.v1i3.2013reponame:Journal of Health & Biological Sciencesinstname:Centro Universitário Christus (Unichristus)instacron:CHRISTUSenghttps://periodicos.unichristus.edu.br/jhbs/article/view/27/30América do SulAmostra de conveniência - demanda espontâneaDireitos autorais 2016 Journal of Health & Biological Scienceshttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2017-06-30T21:23:41Zoai:ojs.unichristus.emnuvens.com.br:article/27Revistahttps://periodicos.unichristus.edu.br/jhbs/indexPRIhttps://periodicos.unichristus.edu.br/jhbs/oaisecretaria.jhbs@unichristus.edu.br || editor.jhbs@fchristus.edu.br2317-30762317-3084opendoar:2023-01-13T09:47:32.180817Journal of Health & Biological Sciences - Centro Universitário Christus (Unichristus)true |
dc.title.none.fl_str_mv |
Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood cultures |
title |
Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood cultures |
spellingShingle |
Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood cultures Leal, Nilma Cintra Saúde; Ciências Biológicas; Microbiologia; Genética de Micro-organismos Listeria; Listeriose; Listeria monocytogenes; Endocardite; Virulência QW 25.5.B2 |
title_short |
Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood cultures |
title_full |
Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood cultures |
title_fullStr |
Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood cultures |
title_full_unstemmed |
Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood cultures |
title_sort |
Biochemical and molecular characteristics of Listeria monocytogenes isolates from a prosthetic mitral heart valve-bearing patient´s blood cultures |
author |
Leal, Nilma Cintra |
author_facet |
Leal, Nilma Cintra Costa, Ana Paula Rocha da Vilela, Marinalda Anselmo Mendes-Marques, Carina Lucena Almeida, Alzira Maria Paiva de |
author_role |
author |
author2 |
Costa, Ana Paula Rocha da Vilela, Marinalda Anselmo Mendes-Marques, Carina Lucena Almeida, Alzira Maria Paiva de |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Leal, Nilma Cintra Costa, Ana Paula Rocha da Vilela, Marinalda Anselmo Mendes-Marques, Carina Lucena Almeida, Alzira Maria Paiva de |
dc.subject.por.fl_str_mv |
Saúde; Ciências Biológicas; Microbiologia; Genética de Micro-organismos Listeria; Listeriose; Listeria monocytogenes; Endocardite; Virulência QW 25.5.B2 |
topic |
Saúde; Ciências Biológicas; Microbiologia; Genética de Micro-organismos Listeria; Listeriose; Listeria monocytogenes; Endocardite; Virulência QW 25.5.B2 |
description |
Background: In Brazil, listeriosis is not a notifiable disease; thus, the incidence of Brazilian cases remains unknown. Listeria monocytogenes is not always included in automated systems, and its detection depends on the high skill level of microbiology laboratory professionals. This paper describes the characteristics of L. monocytogenes isolates fortuitously obtained from an endocarditis case in Recife, PE, Brazil. Methods: Six bacterial isolates obtained from six blood cultures from a 28-year-old male bearing a prosthetic mitral heart valve were analyzed by PCR using primers specific of L. monocytogenes to confirm a presumptive identification, determine the serotype and presence of the virulence genes (inlA, inlB, inlC, inlJ, hly, plcA, actA, prfA) in an attempt to determine the Listeria genotype by PCR-ribotyping. Results: The samples were identified as L. monocytogenes 4b. All investigated virulence genes were amplified by PCR, and the identity of the amplified segments was confirmed by sequencing. A deletion of 105 base pairs was detected in the actA gene. All of the samples generated the same PCR-ribotype pattern, clustered into a single ribotype, and were considered a single strain. Conclusion: L. monocytogenes infection should be considered in endocarditis differential diagnoses, especially among high-risk groups, due to its high pathogenicity and the environmental ubiquity. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por Pares Peer Review Revisado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.unichristus.edu.br/jhbs/article/view/27 10.12662/2317-3076jhbs.v1i3.27.p116.2013 |
url |
https://periodicos.unichristus.edu.br/jhbs/article/view/27 |
identifier_str_mv |
10.12662/2317-3076jhbs.v1i3.27.p116.2013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.unichristus.edu.br/jhbs/article/view/27/30 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2016 Journal of Health & Biological Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2016 Journal of Health & Biological Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
América do Sul Amostra de conveniência - demanda espontânea |
dc.publisher.none.fl_str_mv |
Instituto para o Desenvolvimento da Educacao |
publisher.none.fl_str_mv |
Instituto para o Desenvolvimento da Educacao |
dc.source.none.fl_str_mv |
Journal of Health & Biological Sciences; v. 1, n. 3 (2013): Journal of Health & Biological Sciences; 116 Journal of Health & Biological Sciences; v. 1, n. 3 (2013): Journal of Health & Biological Sciences; 116 Journal of Health and Biological Sciences; v. 1, n. 3 (2013): Journal of Health & Biological Sciences; 116 2317-3076 2317-3084 10.12662/2317-3076jhbs.v1i3.2013 reponame:Journal of Health & Biological Sciences instname:Centro Universitário Christus (Unichristus) instacron:CHRISTUS |
instname_str |
Centro Universitário Christus (Unichristus) |
instacron_str |
CHRISTUS |
institution |
CHRISTUS |
reponame_str |
Journal of Health & Biological Sciences |
collection |
Journal of Health & Biological Sciences |
repository.name.fl_str_mv |
Journal of Health & Biological Sciences - Centro Universitário Christus (Unichristus) |
repository.mail.fl_str_mv |
secretaria.jhbs@unichristus.edu.br || editor.jhbs@fchristus.edu.br |
_version_ |
1797052857057280000 |