ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Científica do CRO-RJ (Online) |
Texto Completo: | https://cro-rj.org.br/revcientifica/index.php/revista/article/view/127 |
Resumo: | Objective: To evaluate the antimicrobial activity of antibiotic pastes used in lesion sterilization and tissue repair (LSTR) technique, through a novel membrane direct contact methodology against a multispecies biofilm and to establish appropriate dilutions for this method. Methods: CTZ (chloramphenicol, tetracycline, zinc oxide) and two formulations of 3Mix pastes (ciprofloxacin, metronidazole, and minocycline), 3Mix1 and 3Mix3, were evaluated with negative (0.9% saline) and positive (chlorhexidine 0.2%) control groups. Candida albicans and Enterococcus faecalis (24-hour) biofilms (n=10) grown on cellulose membranes were directlyexposed to standardized amounts of fresh pastes and control solutions (n=2) for 24h. Membranes were immersed in 900 ?l of saline solution, and seven serial dilutions were made for each sample. Plating for each dilution (n=2) was performed on culture media for microbial colony-forming unit (CFU) counting of totalmicroorganisms, Candida spp. and Enterococcus spp. Aiming the comparison between groups, CFU quantification data were transformed into log10 CFU / mL and the Mann-Whitney test was applied (p<0.05). Results: Inhibition of CFU was observed for all pastes, with greatest effects for CTZ paste in medium selective for Candida spp. (p<0.001) and 3Mix1 in non-selective (p<0.000) and selective for Enterococcus spp. (p<0.004). Conclusion: The pastes showed antimicrobial activity against the tested multispecies biofilm, and the proposed direct contact methodology was efficient. Moreover, the dilutions used proved to be appropriatefor this methodology. |
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ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDYANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDYdente decíduotratamento de canal radicularcombinação de medicamentosagentes antibacterianostestes de sensibilidade microbianadeciduous toothroot canal treatmentdrug combinationantibacterial agentsmicrobial sensitivity testsObjective: To evaluate the antimicrobial activity of antibiotic pastes used in lesion sterilization and tissue repair (LSTR) technique, through a novel membrane direct contact methodology against a multispecies biofilm and to establish appropriate dilutions for this method. Methods: CTZ (chloramphenicol, tetracycline, zinc oxide) and two formulations of 3Mix pastes (ciprofloxacin, metronidazole, and minocycline), 3Mix1 and 3Mix3, were evaluated with negative (0.9% saline) and positive (chlorhexidine 0.2%) control groups. Candida albicans and Enterococcus faecalis (24-hour) biofilms (n=10) grown on cellulose membranes were directlyexposed to standardized amounts of fresh pastes and control solutions (n=2) for 24h. Membranes were immersed in 900 ?l of saline solution, and seven serial dilutions were made for each sample. Plating for each dilution (n=2) was performed on culture media for microbial colony-forming unit (CFU) counting of totalmicroorganisms, Candida spp. and Enterococcus spp. Aiming the comparison between groups, CFU quantification data were transformed into log10 CFU / mL and the Mann-Whitney test was applied (p<0.05). Results: Inhibition of CFU was observed for all pastes, with greatest effects for CTZ paste in medium selective for Candida spp. (p<0.001) and 3Mix1 in non-selective (p<0.000) and selective for Enterococcus spp. (p<0.004). Conclusion: The pastes showed antimicrobial activity against the tested multispecies biofilm, and the proposed direct contact methodology was efficient. Moreover, the dilutions used proved to be appropriatefor this methodology.Objetivo: Avaliar a atividade antimicrobiana de pastas antibióticas utilizadas na técnica Lesion Sterilization and Tissue Repair (LSTR), através de nova metodologia de contato direto com membrana contra um biofilme multiespécies e estabelecer diluições adequadas para avaliação. Métodos: CTZ (cloranfenicol, tetraciclina,óxido de zinco) e duas formulações de pastas 3Mix (Ciprofloxacina, Metronidazol e Minociclina), 3Mix1 e 3Mix3, foram avaliadas, além dos grupos controle, negativo (solução salina a 0,9%) e positivo (clorexidina 0,2%). Biofilmes de Candida albicans e Enterococcus faecalis cultivados sobre membranas de celulose (n=10) durante 24 h foram expostos diretamente em contato com quantidades padronizadas de pastas frescas e controles (n = 2) por 24 h. As membranas foram imersas em 900 ?L de solução salina e sete diluições seriadas foram obtidas por amostra. O plaqueamento para cada diluição (n = 2) foi realizado em meios de cultura para microrganismos totais e seletivos para Candida spp. e Enterococcus spp. para contagem de unidades formadoras de colônias (UFC). Para comparação entre grupos, os dados da contagem de UFC foram convertidos em log10 UFC / mL e o teste Mann-Whitney foi aplicado (p<0,05). Resultados: Observou-se inibição de UFC para todas as pastas, maior para CTZ no meio seletivo para Candida (p<0,001) e 3Mix1 nos demais meios (p<0,004). Conclusão: Concluiu-se que as pastas apresentaram atividade antimicrobiana contra o biofilme multiespécies testado e que a nova metodologia de contato direto proposta foi eficiente. Além disso, as diluições utilizadas mostraram-se adequadas para essa metodologia.Rio de Janeiro Dental Journal2020-10-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://cro-rj.org.br/revcientifica/index.php/revista/article/view/12710.29327/24816.5.1-4Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); Vol. 5 No. 1: January-Abril 2020; 17-23Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); v. 5 n. 1: January-Abril 2020; 17-232595-47331518-524910.29327/24816.5.1reponame:Revista Científica do CRO-RJ (Online)instname:Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ)instacron:CROporhttps://cro-rj.org.br/revcientifica/index.php/revista/article/view/127/91Copyright (c) 2020 Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal)info:eu-repo/semantics/openAccessSancas, Marianade Souza, Anne Caroline LunardiMonteiro, Amanda Souza NunnesPintor, Andréa Vaz BragaDuarte, Maysa LannesPrimo, Laura Salignac de Souza Guimarães2021-06-11T14:17:24Zoai:ojs3.cro-rj.org.br:article/127Revistahttps://cro-rj.org.br/revcientifica/index.php/revistahttps://cro-rj.org.br/revcientifica/index.php/revista/oairevista.cientifica@cro-rj.org.br || rorefa@terra.com.br2595-47331518-5249opendoar:2021-06-11T14:17:24Revista Científica do CRO-RJ (Online) - Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ)false |
dc.title.none.fl_str_mv |
ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY |
title |
ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY |
spellingShingle |
ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY Sancas, Mariana dente decíduo tratamento de canal radicular combinação de medicamentos agentes antibacterianos testes de sensibilidade microbiana deciduous tooth root canal treatment drug combination antibacterial agents microbial sensitivity tests |
title_short |
ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY |
title_full |
ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY |
title_fullStr |
ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY |
title_full_unstemmed |
ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY |
title_sort |
ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY |
author |
Sancas, Mariana |
author_facet |
Sancas, Mariana de Souza, Anne Caroline Lunardi Monteiro, Amanda Souza Nunnes Pintor, Andréa Vaz Braga Duarte, Maysa Lannes Primo, Laura Salignac de Souza Guimarães |
author_role |
author |
author2 |
de Souza, Anne Caroline Lunardi Monteiro, Amanda Souza Nunnes Pintor, Andréa Vaz Braga Duarte, Maysa Lannes Primo, Laura Salignac de Souza Guimarães |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Sancas, Mariana de Souza, Anne Caroline Lunardi Monteiro, Amanda Souza Nunnes Pintor, Andréa Vaz Braga Duarte, Maysa Lannes Primo, Laura Salignac de Souza Guimarães |
dc.subject.por.fl_str_mv |
dente decíduo tratamento de canal radicular combinação de medicamentos agentes antibacterianos testes de sensibilidade microbiana deciduous tooth root canal treatment drug combination antibacterial agents microbial sensitivity tests |
topic |
dente decíduo tratamento de canal radicular combinação de medicamentos agentes antibacterianos testes de sensibilidade microbiana deciduous tooth root canal treatment drug combination antibacterial agents microbial sensitivity tests |
description |
Objective: To evaluate the antimicrobial activity of antibiotic pastes used in lesion sterilization and tissue repair (LSTR) technique, through a novel membrane direct contact methodology against a multispecies biofilm and to establish appropriate dilutions for this method. Methods: CTZ (chloramphenicol, tetracycline, zinc oxide) and two formulations of 3Mix pastes (ciprofloxacin, metronidazole, and minocycline), 3Mix1 and 3Mix3, were evaluated with negative (0.9% saline) and positive (chlorhexidine 0.2%) control groups. Candida albicans and Enterococcus faecalis (24-hour) biofilms (n=10) grown on cellulose membranes were directlyexposed to standardized amounts of fresh pastes and control solutions (n=2) for 24h. Membranes were immersed in 900 ?l of saline solution, and seven serial dilutions were made for each sample. Plating for each dilution (n=2) was performed on culture media for microbial colony-forming unit (CFU) counting of totalmicroorganisms, Candida spp. and Enterococcus spp. Aiming the comparison between groups, CFU quantification data were transformed into log10 CFU / mL and the Mann-Whitney test was applied (p<0.05). Results: Inhibition of CFU was observed for all pastes, with greatest effects for CTZ paste in medium selective for Candida spp. (p<0.001) and 3Mix1 in non-selective (p<0.000) and selective for Enterococcus spp. (p<0.004). Conclusion: The pastes showed antimicrobial activity against the tested multispecies biofilm, and the proposed direct contact methodology was efficient. Moreover, the dilutions used proved to be appropriatefor this methodology. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://cro-rj.org.br/revcientifica/index.php/revista/article/view/127 10.29327/24816.5.1-4 |
url |
https://cro-rj.org.br/revcientifica/index.php/revista/article/view/127 |
identifier_str_mv |
10.29327/24816.5.1-4 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://cro-rj.org.br/revcientifica/index.php/revista/article/view/127/91 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Rio de Janeiro Dental Journal |
publisher.none.fl_str_mv |
Rio de Janeiro Dental Journal |
dc.source.none.fl_str_mv |
Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); Vol. 5 No. 1: January-Abril 2020; 17-23 Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); v. 5 n. 1: January-Abril 2020; 17-23 2595-4733 1518-5249 10.29327/24816.5.1 reponame:Revista Científica do CRO-RJ (Online) instname:Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ) instacron:CRO |
instname_str |
Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ) |
instacron_str |
CRO |
institution |
CRO |
reponame_str |
Revista Científica do CRO-RJ (Online) |
collection |
Revista Científica do CRO-RJ (Online) |
repository.name.fl_str_mv |
Revista Científica do CRO-RJ (Online) - Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ) |
repository.mail.fl_str_mv |
revista.cientifica@cro-rj.org.br || rorefa@terra.com.br |
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1797042306269839360 |