Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil

Detalhes bibliográficos
Autor(a) principal: Trilles, L.
Data de Publicação: 2003
Outros Autores: Lazéra, M., Wanke, B., Theelen, B., Boekhout, T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33608
Resumo: 2020-06-20
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spelling Trilles, L.Lazéra, M.Wanke, B.Theelen, B.Boekhout, T.2019-06-20T18:18:36Z2019-06-20T18:18:36Z2003TRILLES, L. et al. Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil.Medical Mycology, v. 41, p. 383-390, 2003.1369-3786https://www.arca.fiocruz.br/handle/icict/3360810.1080/13693780310001372061460-2709engOxford University PressCryptococcus gattiiCryptococcus neoformansEcologyGenotypingTreesYeastGenetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-06-20Fundação Oswaldo Cruz. lnstituto de Pesquisa Clinica Evandro Chagas. Serviqo de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. lnstituto de Pesquisa Clinica Evandro Chagas. Serviqo de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. lnstituto de Pesquisa Clinica Evandro Chagas. Serviqo de Micologia. Rio de Janeiro, RJ, Brasil.Centraalbureau voor Schimmelcultures. Utrecht, The Netherlands.Centraalbureau voor Schimmelcultures. Utrecht, The Netherlands.The genetic affiliation of a large number of isolates of the Cryptococcus neofornqans species complex from environmental sources in Brazil has been investigated using amplified fragment length polymorphism (AFLP). The strains of C. neoformans isolated from a single tree, as well as from neighbouring trees, showed high similarity values ( > 95%) of their AFLP patterns, thus suggesting considerable genetic homogeneity. The majority of isolates of C. neoforrnans belonged to AFLP genotype 1, and had serotype A and mating type a ( = C. neoformans var. grubii). Three isolates belonged to AFLP genotype 2, with serotype D and mating type a ( = C. neofornzans var. neoformans). One isolate, obtained from a building in Rio de Janeiro inhabited by pigeons, belonged to the AD hybrid AFLP genotype 3. All isolates from trees of C. neoforrnans var. gattii ( = C. gattii) belonged to AFLP genotype 6, and their banding patterns showed relatively low genetic homogeneity with a similarity value of about 76%. Isolates of this genotype occupy an environmental niche in the Americas, and they may cause disease in non-AIDS and AIDS patients as well.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33608/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Trilles_L_etal_INI_2003.pdfve_Trilles_L_etal_INI_2003.pdfapplication/pdf2722736https://www.arca.fiocruz.br/bitstream/icict/33608/2/ve_Trilles_L_etal_INI_2003.pdf003015e446379583e7ddcd53d619f3a4MD52TEXTve_Trilles_L_etal_INI_2003.pdf.txtve_Trilles_L_etal_INI_2003.pdf.txtExtracted texttext/plain27828https://www.arca.fiocruz.br/bitstream/icict/33608/3/ve_Trilles_L_etal_INI_2003.pdf.txt8dbe369fcb5bb7ad469b97b434a5a206MD53icict/336082021-03-24 16:32:33.215oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil
title Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil
spellingShingle Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil
Trilles, L.
Cryptococcus gattii
Cryptococcus neoformans
Ecology
Genotyping
Trees
Yeast
title_short Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil
title_full Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil
title_fullStr Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil
title_full_unstemmed Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil
title_sort Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil
author Trilles, L.
author_facet Trilles, L.
Lazéra, M.
Wanke, B.
Theelen, B.
Boekhout, T.
author_role author
author2 Lazéra, M.
Wanke, B.
Theelen, B.
Boekhout, T.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Trilles, L.
Lazéra, M.
Wanke, B.
Theelen, B.
Boekhout, T.
dc.subject.other.pt_BR.fl_str_mv Cryptococcus gattii
Cryptococcus neoformans
Ecology
Genotyping
Trees
Yeast
topic Cryptococcus gattii
Cryptococcus neoformans
Ecology
Genotyping
Trees
Yeast
description 2020-06-20
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2019-06-20T18:18:36Z
dc.date.available.fl_str_mv 2019-06-20T18:18:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TRILLES, L. et al. Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil.Medical Mycology, v. 41, p. 383-390, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33608
dc.identifier.issn.pt_BR.fl_str_mv 1369-3786
dc.identifier.doi.none.fl_str_mv 10.1080/1369378031000137206
dc.identifier.eissn.none.fl_str_mv 1460-2709
identifier_str_mv TRILLES, L. et al. Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil.Medical Mycology, v. 41, p. 383-390, 2003.
1369-3786
10.1080/1369378031000137206
1460-2709
url https://www.arca.fiocruz.br/handle/icict/33608
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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