Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.

Detalhes bibliográficos
Autor(a) principal: Martins Filho, Olindo Assis
Data de Publicação: 1995
Outros Autores: Pereira, Maria Elizabeth S., Carvalho, Juçara F., Cançado, Joaquim R, Brener, Zigman
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30737
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, Brasil
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spelling Martins Filho, Olindo AssisPereira, Maria Elizabeth S.Carvalho, Juçara F.Cançado, Joaquim RBrener, Zigman2018-12-21T18:03:15Z2018-12-21T18:03:15Z1995MARTINS FILHO, Olindo Assis et al. Flow cytometry:a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease. Clin Diagn Lab Immunol., v. 2, n.5, p. 569-773, 1995.1071-412Xhttps://www.arca.fiocruz.br/handle/icict/30737engAmerican Society for MicrobiologyTrypanosoma cruziDoença de chagasTrypanosoma cruziChagas diseaseFlow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, BrasilSera from patients chronically infected with Trypanosoma cruzi display antibodies that bind to epitopes of living trypomastigotes, known as lytic antibodies (LA), and are detected by a complement-mediated lysis test. Conventional serology antibodies (CSA) are also present in sera from patients with chronic infections but, in contrast to LA, are unable to recognize viable trypomastigotes. The presence of LA has been used as an important element in the criterion of cure in human Chagas' disease. Using flow cytometry technology, we introduced a new and sensitive immunomethod for the detection of anti-live trypomastigote membrane-bound antibodies. On the basis of serological tests (LA and CSA detection) and parasitological assays such as hemoculture (HE), patients were classified into the following groups: chronically infected untreated patients (NT) and treated not-cured patients (TNC), with positive HE and both LA and CSA in their sera; "dissociated" HE-negative patients (DIS), in whom LA was not detected whereas CSA were present; a group of cured HE-negative patients (CUR), who were both LA and CSA negative; and, as control, a group of non-chagasic individuals (NC). Sera from these patients were assayed by incubation with live bloodstream trypomastigotes, which were subsequently exposed to fluorescein isothiocyanate-conjugated anti-human immunoglobulin G. The parasites were then fixed, run in the cytometer, and identified on basis of their size and granularity gain adjustments. On the basis of experience with the complement-mediated lysis test, a level of 20% of parasites being fluorescein isothiocyanate fluorescence positive was used as a cutoff between effective and ineffective treatments. With this criterion, our results indicated that sera from NT and TNC patients were antibody positive whereas all sera from DIS, CUR, and NC patients did not contain membrane-bound antibodies. This new approach is a tool to easily identify anti-live T. cruzi membrane-bound antibodies that can be used to monitor the efficacy of Chagas' diseaseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30737/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALFlow Cytometry, a New Approach To Detect Anti-Live.pdfFlow Cytometry, a New Approach To Detect Anti-Live.pdfapplication/pdf3576008https://www.arca.fiocruz.br/bitstream/icict/30737/2/Flow%20Cytometry%2c%20a%20New%20Approach%20To%20Detect%20Anti-Live.pdf51c9e96b9978f33c8a2ca49742807a23MD52TEXTFlow Cytometry, a New Approach To Detect Anti-Live.pdf.txtFlow Cytometry, a New Approach To Detect Anti-Live.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.
title Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.
spellingShingle Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.
Martins Filho, Olindo Assis
Trypanosoma cruzi
Doença de chagas
Trypanosoma cruzi
Chagas disease
title_short Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.
title_full Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.
title_fullStr Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.
title_full_unstemmed Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.
title_sort Flow cytometry, a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease.
author Martins Filho, Olindo Assis
author_facet Martins Filho, Olindo Assis
Pereira, Maria Elizabeth S.
Carvalho, Juçara F.
Cançado, Joaquim R
Brener, Zigman
author_role author
author2 Pereira, Maria Elizabeth S.
Carvalho, Juçara F.
Cançado, Joaquim R
Brener, Zigman
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Martins Filho, Olindo Assis
Pereira, Maria Elizabeth S.
Carvalho, Juçara F.
Cançado, Joaquim R
Brener, Zigman
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de chagas
topic Trypanosoma cruzi
Doença de chagas
Trypanosoma cruzi
Chagas disease
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chagas disease
description Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, Brasil
publishDate 1995
dc.date.issued.fl_str_mv 1995
dc.date.accessioned.fl_str_mv 2018-12-21T18:03:15Z
dc.date.available.fl_str_mv 2018-12-21T18:03:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS FILHO, Olindo Assis et al. Flow cytometry:a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease. Clin Diagn Lab Immunol., v. 2, n.5, p. 569-773, 1995.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30737
dc.identifier.issn.pt_BR.fl_str_mv 1071-412X
identifier_str_mv MARTINS FILHO, Olindo Assis et al. Flow cytometry:a new approach to detect anti-live trypomastigote antibodies and monitor the efficacy of specific treatment in human Chagas' disease. Clin Diagn Lab Immunol., v. 2, n.5, p. 569-773, 1995.
1071-412X
url https://www.arca.fiocruz.br/handle/icict/30737
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/30737/1/license.txt
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