Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis

Detalhes bibliográficos
Autor(a) principal: Romanos, Maria T. V.
Data de Publicação: 2011
Outros Autores: Andrada-Serpa, Maria J., Mourão, Paulo A. S., Yoneshigue-Valentin, Yocie, Pereira, Mariana S., Santos, Norma, Wigg, Marcia D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36954
Resumo: 2020-11-08
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spelling Romanos, Maria T. V.Andrada-Serpa, Maria J.Mourão, Paulo A. S.Yoneshigue-Valentin, YociePereira, Mariana S.Santos, NormaWigg, Marcia D.2019-11-08T13:18:10Z2019-11-08T13:18:10Z2011ROMANOS, Maria T. V. et al. Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis. Revista Brasileira de Farmacognosia, v. 21, n. 2, p. 229-233, Mar./Apr. 2011.0102-695Xhttps://www.arca.fiocruz.br/handle/icict/3695410.1590/S0102-695X20110050000551981-528XengElsevierSulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-11-08Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa de Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Tecido Conjuntivo. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Botânica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Tecido Conjuntivo. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.A sulfated fucan from Laminaria abyssalis marine alga prevented the interaction of HTLV-1 particles, purified from the MT-2 cell line, with HeLa cells. The infection obtained using a concentrated virus suspension was detected only by amplification of the newly synthesized HTLV-1 proviral cDNA by the nestedpolymerase chain reaction (PCR). The sulfated polysaccharide was not toxic to the cells at a concentration of 100 µg/mL and prevented infection by the viral particles when added to the cell monolayers. The proviral cDNA was only detected when the sulfated polysaccharide was added to the cells three hours post-infection, indicating that the inhibitory activity occurred in the initial stages of virus-cell interaction. Our results demonstrate, for the first time, the ability of a sulfated fucan from marine algae to inhibit virus transmission through free virus particles.HTLV-1Free particlesLaminaria abyssalisMarine algaeSulfated fucanAntiviral activityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/36954/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Romanos_Maria_etal_INI_2011.pdfve_Romanos_Maria_etal_INI_2011.pdfapplication/pdf932660https://www.arca.fiocruz.br/bitstream/icict/36954/2/ve_Romanos_Maria_etal_INI_2011.pdf6ecb355c34d261d03443d87df24dad21MD52TEXTve_Romanos_Maria_etal_INI_2011.pdf.txtve_Romanos_Maria_etal_INI_2011.pdf.txtExtracted texttext/plain21929https://www.arca.fiocruz.br/bitstream/icict/36954/3/ve_Romanos_Maria_etal_INI_2011.pdf.txtfc9e5d588c2ea94400dd3e771f5758b5MD53icict/369542021-03-24 16:32:38.747oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
title Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
spellingShingle Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
Romanos, Maria T. V.
HTLV-1
Free particles
Laminaria abyssalis
Marine algae
Sulfated fucan
Antiviral activity
title_short Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
title_full Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
title_fullStr Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
title_full_unstemmed Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
title_sort Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
author Romanos, Maria T. V.
author_facet Romanos, Maria T. V.
Andrada-Serpa, Maria J.
Mourão, Paulo A. S.
Yoneshigue-Valentin, Yocie
Pereira, Mariana S.
Santos, Norma
Wigg, Marcia D.
author_role author
author2 Andrada-Serpa, Maria J.
Mourão, Paulo A. S.
Yoneshigue-Valentin, Yocie
Pereira, Mariana S.
Santos, Norma
Wigg, Marcia D.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Romanos, Maria T. V.
Andrada-Serpa, Maria J.
Mourão, Paulo A. S.
Yoneshigue-Valentin, Yocie
Pereira, Mariana S.
Santos, Norma
Wigg, Marcia D.
dc.subject.en.pt_BR.fl_str_mv HTLV-1
Free particles
Laminaria abyssalis
Marine algae
Sulfated fucan
Antiviral activity
topic HTLV-1
Free particles
Laminaria abyssalis
Marine algae
Sulfated fucan
Antiviral activity
description 2020-11-08
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2019-11-08T13:18:10Z
dc.date.available.fl_str_mv 2019-11-08T13:18:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROMANOS, Maria T. V. et al. Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis. Revista Brasileira de Farmacognosia, v. 21, n. 2, p. 229-233, Mar./Apr. 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36954
dc.identifier.issn.pt_BR.fl_str_mv 0102-695X
dc.identifier.doi.none.fl_str_mv 10.1590/S0102-695X2011005000055
dc.identifier.eissn.none.fl_str_mv 1981-528X
identifier_str_mv ROMANOS, Maria T. V. et al. Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis. Revista Brasileira de Farmacognosia, v. 21, n. 2, p. 229-233, Mar./Apr. 2011.
0102-695X
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url https://www.arca.fiocruz.br/handle/icict/36954
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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