The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes

Detalhes bibliográficos
Autor(a) principal: Avelar, Bethânia Alves de
Data de Publicação: 2011
Outros Autores: Lélis, Felipe J. N., Avelar, Renato Sathler, Weber, Mathias, Fagundes, Elaine Maria de Souza, Lopes, Miriam Teresa Paz, Martins Filho, Olindo Assis, Melo, Gustavo Eustáquio Brito Alvim de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59561
Resumo: Universidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil.
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spelling Avelar, Bethânia Alves deLélis, Felipe J. N.Avelar, Renato SathlerWeber, MathiasFagundes, Elaine Maria de SouzaLopes, Miriam Teresa PazMartins Filho, Olindo AssisMelo, Gustavo Eustáquio Brito Alvim de2023-07-13T13:25:12Z2023-07-13T13:25:12Z2011AVELAR, Bethânia Alves et al. The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4+ T lymphocytes. Revista Brasileira de Farmacognosia, v. 21, n. 4, p. 662-667, Jul./Aug. 2011.0102-695Xhttps://www.arca.fiocruz.br/handle/icict/5956110.1590/S0102-695X2011005000096engSociedade Brasileira de FarmacognosiaThe crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores. Belo Horizonte, MG, Brazil.Universidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores. Belo Horizonte, MG, Brazil.Universidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil.The plants of the Euphorbiaceae family, especially those of the genus Euphorbia, are frequently used by Brazilian folk communities to treat a wide variety of infectious, tumoral and inflammatory illnesses. Among the species of this genus, Euphorbia tirucalli L. is widely used in some Brazilian regions, such as the Jequitinhonha River Valley. There is evidence that the latex produced by E. tirucalli has antiviral and antitumor activities, but little is known about the mechanisms involved in these effects. It is likely that the mechanism for such activities involves leukocyte activation and cytokine production. In this work, we aimed to evaluate the production of type 1 (TNF-alpha and IFN-gamma) and type 2 (IL-4 and IL-10) cytokines by circulating leukocyte subsets submitted to brief stimulation with the crude latex of E. tirucalli. Peripheral blood leukocytes of twenty healthy subjects were submitted to 4 h incubation with crude E. tirucalli latex diluted in dimethylsulfoxide. After the incubation period, the cells were stained with FITC-conjugated monoclonal antibodies specific to the cell surface receptors CD4, CD8 and CD14, and to PE-conjugated monoclonal antibodies specific to the cytokines TNF-alpha, IFN-gamma, IL-4 and IL-10. The acquisition and analysis of data were performed by flow cytometry. The results showed a significant increase (p<0.05) in the percentage of CD4(+) T lymphocytes positive for the type 1 cytokines TNF-alpha and IFN-gamma. Neutrophils and CD8(+) T lymphocytes showed a mixed profile of cytokine production, characterized by an increase in the percentage of cells expressing IFN-gamma, TNF-alpha, and IL-10. The data indicate a predominant type 1 cytokine response. The findings presented suggest that the effect popularly attributed to E. tirucalli usage may be attributed to its effect on the production of TNF-alpha and IFN-gamma. However, the relationship between the in vitro and in vivo effects of E. tirucalli needs to be investigated.Euphorbia tirucalliMedicinal plantType 1 cytokinesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALArticle 14.indd.pdfArticle 14.indd.pdfapplication/pdf424420https://www.arca.fiocruz.br/bitstream/icict/59561/2/Article%2014.indd.pdf51a3b74e7b03145329b3acd92f97c704MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59561/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/595612023-07-13 10:49:26.161oai:www.arca.fiocruz.br:icict/59561Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-13T13:49:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes
title The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes
spellingShingle The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes
Avelar, Bethânia Alves de
Euphorbia tirucalli
Medicinal plant
Type 1 cytokines
title_short The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes
title_full The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes
title_fullStr The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes
title_full_unstemmed The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes
title_sort The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes
author Avelar, Bethânia Alves de
author_facet Avelar, Bethânia Alves de
Lélis, Felipe J. N.
Avelar, Renato Sathler
Weber, Mathias
Fagundes, Elaine Maria de Souza
Lopes, Miriam Teresa Paz
Martins Filho, Olindo Assis
Melo, Gustavo Eustáquio Brito Alvim de
author_role author
author2 Lélis, Felipe J. N.
Avelar, Renato Sathler
Weber, Mathias
Fagundes, Elaine Maria de Souza
Lopes, Miriam Teresa Paz
Martins Filho, Olindo Assis
Melo, Gustavo Eustáquio Brito Alvim de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Avelar, Bethânia Alves de
Lélis, Felipe J. N.
Avelar, Renato Sathler
Weber, Mathias
Fagundes, Elaine Maria de Souza
Lopes, Miriam Teresa Paz
Martins Filho, Olindo Assis
Melo, Gustavo Eustáquio Brito Alvim de
dc.subject.en.en_US.fl_str_mv Euphorbia tirucalli
Medicinal plant
Type 1 cytokines
topic Euphorbia tirucalli
Medicinal plant
Type 1 cytokines
description Universidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2023-07-13T13:25:12Z
dc.date.available.fl_str_mv 2023-07-13T13:25:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AVELAR, Bethânia Alves et al. The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4+ T lymphocytes. Revista Brasileira de Farmacognosia, v. 21, n. 4, p. 662-667, Jul./Aug. 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59561
dc.identifier.issn.en_US.fl_str_mv 0102-695X
dc.identifier.doi.none.fl_str_mv 10.1590/S0102-695X2011005000096
identifier_str_mv AVELAR, Bethânia Alves et al. The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4+ T lymphocytes. Revista Brasileira de Farmacognosia, v. 21, n. 4, p. 662-667, Jul./Aug. 2011.
0102-695X
10.1590/S0102-695X2011005000096
url https://www.arca.fiocruz.br/handle/icict/59561
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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