Analysing ambiguities in trypanosomatids taxonomy by barcoding
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42395 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários da Fiocruz. Rio de Janeiro, RJ, Brasil. |
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Boucinha, CarolinaCaetano, Amanda R.Santos, Helena L. C.Helaers, RaphaelVikkula, MiikkaBranquinha, Marta HelenaSantos, André Luis Souza dosGrellier, PhilippeMorelli, Karina Alessandrad'Avila-Levy, Claudia Masini2020-07-25T18:05:30Z2020-07-25T18:05:30Z2020BOUCINHA, Carolina et al. Analysing ambiguities in trypanosomatids taxonomy by barcoding. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, p. 1-14, 2020.0074-0276https://www.arca.fiocruz.br/handle/icict/4239510.1590/0074-027602005041678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.BarrasCodificação de barrasKinetoplasteaTaxonomiaTrypanosomatidaeBarcodeBarcodingKinetoplasteaTaxonomyTrypanosomatidaTrypanosomatidaeAnalysing ambiguities in trypanosomatids taxonomy by barcodinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários da Fiocruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários da Fiocruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários da Fiocruz. Rio de Janeiro, RJ, Brasil.University of Louvain. de Duve Institute. Laboratory of Human Molecular Genetics. Brussels, Belgium.University of Louvain. de Duve Institute. Laboratory of Human Molecular Genetics. Brussels, Belgium.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Muséum National d‘Histoire Naturelle. Unité Molécules de Communication et Adaptation des Microorganisme. Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários da Fiocruz. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Ecologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários da Fiocruz. Rio de Janeiro, RJ, Brasil / University of Louvain. de Duve Institute. Laboratory of Human Molecular Genetics. Brussels, Belgium.BACKGROUND: Biodiversity screens and phylogenetic studies are dependent on reliable DNA sequences in public databases. Biological collections possess vouchered specimens with a traceable history. Therefore, DNA sequencing of samples available at institutional collections can greatly contribute to taxonomy, and studies on evolution and biodiversity. METHODS: We sequenced part of the glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) and the SSU rRNA (V7/V8) genes from 102 trypanosomatid cultures, which are available on request at www.colprot.fiocruz.br. OBJECTIVE: The main objective of this work was to use phylogenetic inferences, using the obtained DNA sequences and those from representatives of all Trypanosomatidae genera, to generate phylogenetic trees that can simplify new isolates screenings. FINDINGS: A DNA sequence is provided for the first time for several isolates, the phylogenetic analysis allowed the classification or reclassification of several specimens, identification of candidates for new genera and species, as well as the taxonomic validation of several deposits. MAIN CONCLUSIONS: This survey aimed at presenting a list of validated species and their associated DNA sequences combined with a short historical overview of each isolate, which can support taxonomic and biodiversity research and promote culture collections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42395/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCarolina_Boucinha_etal_IOC_2020.pdfCarolina_Boucinha_etal_IOC_2020.pdfapplication/pdf694074https://www.arca.fiocruz.br/bitstream/icict/42395/2/Carolina_Boucinha_etal_IOC_2020.pdfb4ebe13ea1615bba61b75674fc34fc1aMD52TEXTCarolina_Boucinha_etal_IOC_2020.pdf.txtCarolina_Boucinha_etal_IOC_2020.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Analysing ambiguities in trypanosomatids taxonomy by barcoding |
| title |
Analysing ambiguities in trypanosomatids taxonomy by barcoding |
| spellingShingle |
Analysing ambiguities in trypanosomatids taxonomy by barcoding Boucinha, Carolina Barras Codificação de barras Kinetoplastea Taxonomia Trypanosomatidae Barcode Barcoding Kinetoplastea Taxonomy Trypanosomatida Trypanosomatidae |
| title_short |
Analysing ambiguities in trypanosomatids taxonomy by barcoding |
| title_full |
Analysing ambiguities in trypanosomatids taxonomy by barcoding |
| title_fullStr |
Analysing ambiguities in trypanosomatids taxonomy by barcoding |
| title_full_unstemmed |
Analysing ambiguities in trypanosomatids taxonomy by barcoding |
| title_sort |
Analysing ambiguities in trypanosomatids taxonomy by barcoding |
| author |
Boucinha, Carolina |
| author_facet |
Boucinha, Carolina Caetano, Amanda R. Santos, Helena L. C. Helaers, Raphael Vikkula, Miikka Branquinha, Marta Helena Santos, André Luis Souza dos Grellier, Philippe Morelli, Karina Alessandra d'Avila-Levy, Claudia Masini |
| author_role |
author |
| author2 |
Caetano, Amanda R. Santos, Helena L. C. Helaers, Raphael Vikkula, Miikka Branquinha, Marta Helena Santos, André Luis Souza dos Grellier, Philippe Morelli, Karina Alessandra d'Avila-Levy, Claudia Masini |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Boucinha, Carolina Caetano, Amanda R. Santos, Helena L. C. Helaers, Raphael Vikkula, Miikka Branquinha, Marta Helena Santos, André Luis Souza dos Grellier, Philippe Morelli, Karina Alessandra d'Avila-Levy, Claudia Masini |
| dc.subject.other.pt_BR.fl_str_mv |
Barras Codificação de barras Kinetoplastea Taxonomia Trypanosomatidae |
| topic |
Barras Codificação de barras Kinetoplastea Taxonomia Trypanosomatidae Barcode Barcoding Kinetoplastea Taxonomy Trypanosomatida Trypanosomatidae |
| dc.subject.en.pt_BR.fl_str_mv |
Barcode Barcoding Kinetoplastea Taxonomy Trypanosomatida Trypanosomatidae |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários da Fiocruz. Rio de Janeiro, RJ, Brasil. |
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2020 |
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2020-07-25T18:05:30Z |
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2020-07-25T18:05:30Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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BOUCINHA, Carolina et al. Analysing ambiguities in trypanosomatids taxonomy by barcoding. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, p. 1-14, 2020. |
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https://www.arca.fiocruz.br/handle/icict/42395 |
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0074-0276 |
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10.1590/0074-02760200504 |
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1678-8060 |
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BOUCINHA, Carolina et al. Analysing ambiguities in trypanosomatids taxonomy by barcoding. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 115, p. 1-14, 2020. 0074-0276 10.1590/0074-02760200504 1678-8060 |
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