Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins

Detalhes bibliográficos
Autor(a) principal: Figueredo-Silva, Jose
Data de Publicação: 2010
Outros Autores: Cavalcanti, Carmelita, Montenegro, Luciano Tavares, Norões, Joaquim, Dreyer, Gerusa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28405
Resumo: Universidade Estadual do Piauí. Faculdade de Ciências Médicas. Núcleo de Ensino e Pesquisa em Patologia. Piauí, Brazil.
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spelling Figueredo-Silva, JoseCavalcanti, CarmelitaMontenegro, Luciano TavaresNorões, JoaquimDreyer, Gerusa2018-08-28T14:20:36Z2018-08-28T14:20:36Z2010FIGUEREDO-SILVA, J. et al. Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins. International journal of biomedical science: IJBS, v. 6, n. 2, p. 111–119, jun. 2010.1550-9702https://www.arca.fiocruz.br/handle/icict/28405engWuchereria bancroftifilariose bancroftianaProteína Catiônica de EosinófiloPeroxidase de EosinófiloEosinófilosgranuloma filarialImuno-Histoquímicaf iarias linficasproteína básica principalWuchereria bancroftibancroftian filariasisEosinophil Cationic ProteinEosinophil Peroxidaseeosinophilsfilarial granulomaimmunohistochemistrylymphatic fiariasismajor basic proteinDiethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual do Piauí. Faculdade de Ciências Médicas. Núcleo de Ensino e Pesquisa em Patologia. Piauí, Brazil.Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami (LIKA). Recife, PE, Brasil.Universidade Federal de Pernambuco. Centro de Ciências da Saúde. Departamento de Patologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Centro de Ciências da Saúde. Departamento de Cirurgia. Serviço de Urologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Hospital das Clínicas. Núcleo de Ensino Pesquisa e Assistência em Filariose (NEPAF). Recife, PE, Brasil.Universidade Federal de Pernambuco. Hospital das Clínicas. Núcleo de Ensino Pesquisa e Assistência em Filariose (NEPAF). Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.It has been suggested, mostly using in vitro experiments, that defenses against parasites involve mainly activated eosinophils and their toxic proteins, such as major basic protein (MBP), eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO). Eosinophil degranulation has been described around degenerating onchocercal microfilariae in patients treated with diethylcarbamazine (DEC). In bancroftian filariasis, traditional histopathologic studies have shown remarkable numbers of eosinophils in granulomatous lesions associated with both DEC-induced and spontaneous death of adult Wuchereria bancrofti parasites. No immunohistochemical study targeting eosinophil degranulation has been previously performed in these granulomas, which are found mainly within intrascrotal lymphatic vessels. This investigation was undertaken in 22 (12 DEC-treated and 10 untreated) male patients in order to determine the immunohistochemical expressions of MBP, EPO and ECP in bancofitian granulomas, using the indirect method. Stained intact esosinophils, as well as granular, extra-cellular material positive for all three proteins, were found in all granulomas. The immunohistochemical patterns were similar in both DEC-treated and untreated cases, irrespective of microfilaremia, blood eosinophilia, and granuloma age. Positive intact cells were observed mostly at the periphery of the granulomas, whereas granular material predominated in central areas around dead or degenerating parasites. These results indicate that eosinophils accumulate in the granulomas and degranulate preferentially in close proximity to degenerating or dead adult parasites. In bancroftian granulomas, influx and degranulation of eosinophils are considered a consequence of parasite death, rather than its cause.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/28405/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALDiethylcarbamazine and Non-Diethylcarbamazine Related.pdfDiethylcarbamazine and Non-Diethylcarbamazine Related.pdfapplication/pdf5415795https://www.arca.fiocruz.br/bitstream/icict/28405/2/Diethylcarbamazine%20and%20Non-Diethylcarbamazine%20Related.pdfabe1e08d5cb9b4a1d72a289919b80e01MD52TEXTDiethylcarbamazine and Non-Diethylcarbamazine Related.pdf.txtDiethylcarbamazine and Non-Diethylcarbamazine Related.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins
title Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins
spellingShingle Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins
Figueredo-Silva, Jose
Wuchereria bancrofti
filariose bancroftiana
Proteína Catiônica de Eosinófilo
Peroxidase de Eosinófilo
Eosinófilos
granuloma filarial
Imuno-Histoquímica
f iarias linficas
proteína básica principal
Wuchereria bancrofti
bancroftian filariasis
Eosinophil Cationic Protein
Eosinophil Peroxidase
eosinophils
filarial granuloma
immunohistochemistry
lymphatic fiariasis
major basic protein
title_short Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins
title_full Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins
title_fullStr Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins
title_full_unstemmed Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins
title_sort Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins
author Figueredo-Silva, Jose
author_facet Figueredo-Silva, Jose
Cavalcanti, Carmelita
Montenegro, Luciano Tavares
Norões, Joaquim
Dreyer, Gerusa
author_role author
author2 Cavalcanti, Carmelita
Montenegro, Luciano Tavares
Norões, Joaquim
Dreyer, Gerusa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Figueredo-Silva, Jose
Cavalcanti, Carmelita
Montenegro, Luciano Tavares
Norões, Joaquim
Dreyer, Gerusa
dc.subject.other.pt_BR.fl_str_mv Wuchereria bancrofti
filariose bancroftiana
Proteína Catiônica de Eosinófilo
Peroxidase de Eosinófilo
Eosinófilos
granuloma filarial
Imuno-Histoquímica
f iarias linficas
proteína básica principal
topic Wuchereria bancrofti
filariose bancroftiana
Proteína Catiônica de Eosinófilo
Peroxidase de Eosinófilo
Eosinófilos
granuloma filarial
Imuno-Histoquímica
f iarias linficas
proteína básica principal
Wuchereria bancrofti
bancroftian filariasis
Eosinophil Cationic Protein
Eosinophil Peroxidase
eosinophils
filarial granuloma
immunohistochemistry
lymphatic fiariasis
major basic protein
dc.subject.en.pt_BR.fl_str_mv Wuchereria bancrofti
bancroftian filariasis
Eosinophil Cationic Protein
Eosinophil Peroxidase
eosinophils
filarial granuloma
immunohistochemistry
lymphatic fiariasis
major basic protein
description Universidade Estadual do Piauí. Faculdade de Ciências Médicas. Núcleo de Ensino e Pesquisa em Patologia. Piauí, Brazil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-08-28T14:20:36Z
dc.date.available.fl_str_mv 2018-08-28T14:20:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv FIGUEREDO-SILVA, J. et al. Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins. International journal of biomedical science: IJBS, v. 6, n. 2, p. 111–119, jun. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28405
dc.identifier.issn.pt_BR.fl_str_mv 1550-9702
identifier_str_mv FIGUEREDO-SILVA, J. et al. Diethylcarbamazine and non-diethylcarbamazine related bancroftian granuloma: an immunohistochemical study of eosinophil toxic proteins. International journal of biomedical science: IJBS, v. 6, n. 2, p. 111–119, jun. 2010.
1550-9702
url https://www.arca.fiocruz.br/handle/icict/28405
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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