Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24909 |
Resumo: | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil |
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Nascimento, Dilzamar V.Dellagostin, Odir A.Matos, Denise C. S.McIntosh, DouglasHirata Jr., RaphaelPereira, Geraldo M. B.Guaraldi, Ana Luiza de MattosArmôa, Geraldo R. G.2018-02-22T12:12:49Z2018-02-22T12:12:49Z2017NASCIMENTO, Dilzamar V. et al. Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin. American Journal of Molecular Biology, v.7, p.176-189, 2017.2161-6620https://www.arca.fiocruz.br/handle/icict/249092161-6663engScientific Research Publishing IncToxina DiftéricaCorynebacterium diphtheriaeDifteriaMycobacterium bovis BCGRecombinant BCGDiphtheria Toxin dtb GenePark Williams 8 (PW8)Corynebacterium diphtheriaerDTBPW8pUS977 VectorMycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversidade Federal de Pelotas. Campus Universitário. Núcleo de Biotecnologia. Pelotas, RS, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ. Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilDiphtheria is a fulminant bacterial disease caused by toxigenic strains of Corynebacterium diphtheriae whose local and systemic manifestations are due to the action of the diphtheria toxin (DT). The vaccine which is used to prevent diphtheria worldwide is a toxoid obtained by detoxifying DT. Although associated with high efficacy in the prevention of disease, the current anti- diphtheria vaccine, one of the components of DTP (diphtheria, tetanus and pertussis triple vaccine), may present post vaccination effects such as toxicity and reactogenicity resulting from the presence of contaminants in the vaccine that originated during the process of production and/or detoxification. Therefore, strategies to develop a less toxic and at the same time economically viable vaccine alternatives are needed to improve existing vaccines in use worldwide. In this study, the Moreau substrain of BCG which is used in Brazil as a live vaccine against human tuberculosis was genetically modified to carry and express the gene encoding for the diphtheria toxin fragment B (DTB). As such, the DNA sequence encoding the dtb gene was cloned into the pUS977 shuttle vector for cytoplasmic expression and successfully introduced into BCG cells by electroporation. Mice immunized with recombinant BCG expressing DTB showed seroconversion with the detection of specific antibodies against DTB. Also, rBCGs stably expressing DTB persisted up to 60 days in the absence of selective pressure in mice and cell viability did not change significantly during the period tested. Finally, immune sera from BALB/c mice vaccinated with rBCGpUS977dtb PW8 were preliminarily tested for their capacity of neutralizing the diphtheria toxin in the Vero Cells assay.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24909/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALgeraldo_armoa_etal_IOC_2017.pdfgeraldo_armoa_etal_IOC_2017.pdfapplication/pdf715759https://www.arca.fiocruz.br/bitstream/icict/24909/2/geraldo_armoa_etal_IOC_2017.pdf233c3857f976dd336cedb7de9789c154MD52TEXTgeraldo_armoa_etal_IOC_2017.pdf.txtgeraldo_armoa_etal_IOC_2017.pdf.txtExtracted texttext/plain40656https://www.arca.fiocruz.br/bitstream/icict/24909/3/geraldo_armoa_etal_IOC_2017.pdf.txt2232488f95f624e57e97f5ae774b2c7dMD53icict/249092023-09-05 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| dc.title.pt_BR.fl_str_mv |
Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin |
| title |
Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin |
| spellingShingle |
Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin Nascimento, Dilzamar V. Toxina Diftérica Corynebacterium diphtheriae Difteria Mycobacterium bovis BCG Recombinant BCG Diphtheria Toxin dtb Gene Park Williams 8 (PW8) Corynebacterium diphtheriae rDTBPW8 pUS977 Vector |
| title_short |
Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin |
| title_full |
Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin |
| title_fullStr |
Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin |
| title_full_unstemmed |
Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin |
| title_sort |
Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin |
| author |
Nascimento, Dilzamar V. |
| author_facet |
Nascimento, Dilzamar V. Dellagostin, Odir A. Matos, Denise C. S. McIntosh, Douglas Hirata Jr., Raphael Pereira, Geraldo M. B. Guaraldi, Ana Luiza de Mattos Armôa, Geraldo R. G. |
| author_role |
author |
| author2 |
Dellagostin, Odir A. Matos, Denise C. S. McIntosh, Douglas Hirata Jr., Raphael Pereira, Geraldo M. B. Guaraldi, Ana Luiza de Mattos Armôa, Geraldo R. G. |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nascimento, Dilzamar V. Dellagostin, Odir A. Matos, Denise C. S. McIntosh, Douglas Hirata Jr., Raphael Pereira, Geraldo M. B. Guaraldi, Ana Luiza de Mattos Armôa, Geraldo R. G. |
| dc.subject.other.pt_BR.fl_str_mv |
Toxina Diftérica Corynebacterium diphtheriae Difteria Mycobacterium bovis BCG |
| topic |
Toxina Diftérica Corynebacterium diphtheriae Difteria Mycobacterium bovis BCG Recombinant BCG Diphtheria Toxin dtb Gene Park Williams 8 (PW8) Corynebacterium diphtheriae rDTBPW8 pUS977 Vector |
| dc.subject.en.pt_BR.fl_str_mv |
Recombinant BCG Diphtheria Toxin dtb Gene Park Williams 8 (PW8) Corynebacterium diphtheriae rDTBPW8 pUS977 Vector |
| description |
Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil |
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2017 |
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2017 |
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2018-02-22T12:12:49Z |
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2018-02-22T12:12:49Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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NASCIMENTO, Dilzamar V. et al. Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin. American Journal of Molecular Biology, v.7, p.176-189, 2017. |
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https://www.arca.fiocruz.br/handle/icict/24909 |
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2161-6620 |
| dc.identifier.eissn.none.fl_str_mv |
2161-6663 |
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NASCIMENTO, Dilzamar V. et al. Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin. American Journal of Molecular Biology, v.7, p.176-189, 2017. 2161-6620 2161-6663 |
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Scientific Research Publishing Inc |
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Scientific Research Publishing Inc |
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