Biofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance

Detalhes bibliográficos
Autor(a) principal: Carvalho, Rodrigo Rezende Mires de
Data de Publicação: 2023
Outros Autores: Dias, Carla Silva, Paz, Lucas Nogueira, Fires, Thainá Melo de Lima, Figueira, Cláudio Pereira, Damasceno, Karine Araújo, Pinna, Melissa Hanzen
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/60780
Resumo: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
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spelling Carvalho, Rodrigo Rezende Mires deDias, Carla SilvaPaz, Lucas NogueiraFires, Thainá Melo de LimaFigueira, Cláudio PereiraDamasceno, Karine AraújoPinna, Melissa Hanzen2023-10-11T19:32:16Z2023-10-11T19:32:16Z2023CARVALHO, Rodrigo Rezende Mires de et al. Biofilm formation in vitro by Leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance. Heliyon, v. 9, p. 1-12, 2023.2405-8440https://www.arca.fiocruz.br/handle/icict/6078010.1016/j.heliyon.2023.e13802Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Laboratório de Doenças Bacterianas. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Laboratório de Doenças Bacterianas. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Laboratório de Doenças Bacterianas. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa de Pós-Graduação em Clínica e Cirurgia de Carnívoros Domésticos. Salvador, BA, Brasil.Ministério da Saúde. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa de Pós-Graduação em Zootecnia nos Trópicos. Laboratório de Doenças Bacterianas. Salvador, BA, Brasil.Leptospira interrogans is a biofilm-forming pathogen, however, there are few data involving Brazilian strains isolated from dogs and their antimicrobial sensitivity in planktonic and biofilm forms. The potential for biofilm formation and antimicrobial resistance in naturally infected dogs is a fundamental approach towards disease epidemiology and the establishment of consistent prophylaxis and control measures. The objective of this study was to evaluate in vitro biofilm formation of a reference strain (L. interrogans, sv. Copenhageni L1 130 - L20) and of L. interrogans isolated from dogs (C20, C29, C51, C82), with subsequent evaluation of antimicrobial susceptibility in planktonic and biofilm forms. The semi quantification of biofilm production revealed a dynamic process of development over time, with mature biofilm formation early on the seventh day of incubation. All strains were efficient for in vitro biofilm formation and, in this form, they were considerably more resistant compared to their planktonic form, with MIC90 of 1600 μg/mL for amoxicillin, 800 μg/mL for ampicillin, and >1600 μg/mL for doxycycline and ciprofloxacin. The strains studies were isolated on naturally infected dogs that might act as reservoirs and sentinels for human infections. The potential to antimicrobial resistance together with the close relation between dogs and humans indicates the need for greater actions on disease control and surveillance. Moreover, biofilm formation may contribute to the persistence of Leptospira interrogans in the host and these animals can act as chronic carriers, disseminating the agent in the environment.engElsevierLeptospira sppBiofilmeMicrodiluição em caldoResistência antimicrobianaLeptospira sppBiofilmBroth microdilutionAntimicrobial resistanceLeptospiraBiofilmesBiofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistanceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60780/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCarvalho, Rodrigo R. M. de - Biofilm formation in vitro by Leptospira interrogans strains isolated.pdfCarvalho, Rodrigo R. M. de - Biofilm formation in vitro by Leptospira interrogans strains isolated.pdfapplication/pdf8605996https://www.arca.fiocruz.br/bitstream/icict/60780/2/Carvalho%2c%20Rodrigo%20R.%20M.%20de%20-%20Biofilm%20formation%20in%20vitro%20by%20Leptospira%20interrogans%20strains%20isolated.pdffd1f1dd132be0e7b49ce2d89f8af9256MD52icict/607802023-10-11 16:32:18.179oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-10-11T19:32:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Biofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance
title Biofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance
spellingShingle Biofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance
Carvalho, Rodrigo Rezende Mires de
Leptospira spp
Biofilme
Microdiluição em caldo
Resistência antimicrobiana
Leptospira spp
Biofilm
Broth microdilution
Antimicrobial resistance
Leptospira
Biofilmes
title_short Biofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance
title_full Biofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance
title_fullStr Biofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance
title_full_unstemmed Biofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance
title_sort Biofilm formation in vitro by leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance
author Carvalho, Rodrigo Rezende Mires de
author_facet Carvalho, Rodrigo Rezende Mires de
Dias, Carla Silva
Paz, Lucas Nogueira
Fires, Thainá Melo de Lima
Figueira, Cláudio Pereira
Damasceno, Karine Araújo
Pinna, Melissa Hanzen
author_role author
author2 Dias, Carla Silva
Paz, Lucas Nogueira
Fires, Thainá Melo de Lima
Figueira, Cláudio Pereira
Damasceno, Karine Araújo
Pinna, Melissa Hanzen
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Rodrigo Rezende Mires de
Dias, Carla Silva
Paz, Lucas Nogueira
Fires, Thainá Melo de Lima
Figueira, Cláudio Pereira
Damasceno, Karine Araújo
Pinna, Melissa Hanzen
dc.subject.other.en_US.fl_str_mv Leptospira spp
Biofilme
Microdiluição em caldo
Resistência antimicrobiana
topic Leptospira spp
Biofilme
Microdiluição em caldo
Resistência antimicrobiana
Leptospira spp
Biofilm
Broth microdilution
Antimicrobial resistance
Leptospira
Biofilmes
dc.subject.en.en_US.fl_str_mv Leptospira spp
Biofilm
Broth microdilution
Antimicrobial resistance
dc.subject.decs.en_US.fl_str_mv Leptospira
Biofilmes
description Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-10-11T19:32:16Z
dc.date.available.fl_str_mv 2023-10-11T19:32:16Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Rodrigo Rezende Mires de et al. Biofilm formation in vitro by Leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance. Heliyon, v. 9, p. 1-12, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/60780
dc.identifier.issn.en_US.fl_str_mv 2405-8440
dc.identifier.doi.none.fl_str_mv 10.1016/j.heliyon.2023.e13802
identifier_str_mv CARVALHO, Rodrigo Rezende Mires de et al. Biofilm formation in vitro by Leptospira interrogans strains isolated from naturally infected dogs and their role in antimicrobial resistance. Heliyon, v. 9, p. 1-12, 2023.
2405-8440
10.1016/j.heliyon.2023.e13802
url https://www.arca.fiocruz.br/handle/icict/60780
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/60780/1/license.txt
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