Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus

Detalhes bibliográficos
Autor(a) principal: Mota, Bruno Eduardo Fernandes
Data de Publicação: 2011
Outros Autores: Romero, Nadia Gallardo, Trindade, Giliane, Keckler, M. Shannon, Karem, Kevin, Carroll, Darin, Campos, Marco Antônio da Silva, Vieira, Leda Quercia, Fonseca, Flávio Guimarães da, Ferreira, Paulo César Peregrino, Bonjardim, Claudio Antonio, Damon, Inger K., Kroon, Erna Geessien
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8979
Resumo: Universidade Federal de Minas Gerais. Departamento de Microbiologia. Laboratório de Virus Belo Horizonte, MG, Brazil
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spelling Mota, Bruno Eduardo FernandesRomero, Nadia GallardoTrindade, GilianeKeckler, M. ShannonKarem, KevinCarroll, DarinCampos, Marco Antônio da SilvaVieira, Leda QuerciaFonseca, Flávio Guimarães daFerreira, Paulo César PeregrinoBonjardim, Claudio AntonioDamon, Inger K.Kroon, Erna Geessien2014-11-26T17:38:30Z2014-11-26T17:38:30Z2011MOTA, Bruno Eduardo Fernandes et al. Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus. PLoS One, v. 6, n. 4, p. e18924, 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/897910.1371/journal.pone.0018924engPublic Library of ScienceAdverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Microbiologia. Laboratório de Virus Belo Horizonte, MG, BrazilCenters for Disease Control and Prevention. Poxvirus Program. Atlanta, GA, United States of AmericaUniversidade Federal de Minas Gerais. Departamento de Microbiologia. Laboratório de Virus Belo Horizonte, MG, BrazilCenters for Disease Control and Prevention. Poxvirus Program. Atlanta, GA, United States of AmericaCenters for Disease Control and Prevention. Poxvirus Program. Atlanta, GA, United States of AmericaCenters for Disease Control and Prevention. Poxvirus Program. Atlanta, GA, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, Minas Gerais, BrazilUniversidade Federal de Minas Gerais. Departamento de Microbiologia. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Microbiologia. Laboratório de Virologia Comparada. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Microbiologia. Laboratório de Virus Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Microbiologia. Laboratório de Virus Belo Horizonte, MG, Brazil,Centers for Disease Control and Prevention. Poxvirus Program. Atlanta, GA, United States of AmericaUniversidade Federal de Minas Gerais. Departamento de Microbiologia. Laboratório de Virus Belo Horizonte, MG, BrazilAdverse events upon smallpox vaccination with fully-replicative strains of Vaccinia virus (VACV) comprise an array of clinical manifestations that occur primarily in immunocompromised patients leading to significant host morbidity/mortality. The expansion of immune-suppressed populations and the possible release of Variola virus as a bioterrorist act have given rise to concerns over vaccination complications should more widespread vaccination be reinitiated. Our goal was to evaluate the components of the host immune system that are sufficient to prevent morbidity/mortality in a murine model of tail scarification, which mimics immunological and clinical features of smallpox vaccination in humans. Infection of C57BL/6 wild-type mice led to a strictly localized infection, with complete viral clearance by day 28 p.i. On the other hand, infection of T and B-cell deficient mice (Rag1(-/-)) produced a severe disease, with uncontrolled viral replication at the inoculation site and dissemination to internal organs. Infection of B-cell deficient animals (µMT) produced no mortality. However, viral clearance in µMT animals was delayed compared to WT animals, with detectable viral titers in tail and internal organs late in infection. Treatment of Rag1(-/-) with rabbit hyperimmune anti-vaccinia serum had a subtle effect on the morbidity/mortality of this strain, but it was effective in reduce viral titers in ovaries. Finally, NUDE athymic mice showed a similar outcome of infection as Rag1(-/-), and passive transfer of WT T cells to Rag1(-/-) animals proved fully effective in preventing morbidity/mortality. These results strongly suggest that both T and B cells are important in the immune response to primary VACV infection in mice, and that T-cells are required to control the infection at the inoculation site and providing help for B-cells to produce antibodies, which help to prevent viral dissemination. These insights might prove helpful to better identify individuals with higher risk of complications after infection with poxvirus.Adaptive Immunity/immunologyCD8-Positive T-Lymphocytes/immunologySmallpox/immunologyVaccinia/prevention & controlinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8979/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALAdverse Events Post Smallpox-Vaccination.pdfAdverse Events Post Smallpox-Vaccination.pdfapplication/pdf1077921https://www.arca.fiocruz.br/bitstream/icict/8979/2/Adverse%20Events%20Post%20Smallpox-Vaccination.pdf1b549876e3ff98badb43c683d73820adMD52TEXTAdverse Events Post Smallpox-Vaccination.pdf.txtAdverse Events Post Smallpox-Vaccination.pdf.txtExtracted texttext/plain58423https://www.arca.fiocruz.br/bitstream/icict/8979/3/Adverse%20Events%20Post%20Smallpox-Vaccination.pdf.txt7e40df360a74dba9d7fffaa06f0c9dc4MD53icict/89792019-06-19 10:13:26.773oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:13:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus
title Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus
spellingShingle Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus
Mota, Bruno Eduardo Fernandes
Adaptive Immunity/immunology
CD8-Positive T-Lymphocytes/immunology
Smallpox/immunology
Vaccinia/prevention & control
title_short Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus
title_full Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus
title_fullStr Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus
title_full_unstemmed Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus
title_sort Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus
author Mota, Bruno Eduardo Fernandes
author_facet Mota, Bruno Eduardo Fernandes
Romero, Nadia Gallardo
Trindade, Giliane
Keckler, M. Shannon
Karem, Kevin
Carroll, Darin
Campos, Marco Antônio da Silva
Vieira, Leda Quercia
Fonseca, Flávio Guimarães da
Ferreira, Paulo César Peregrino
Bonjardim, Claudio Antonio
Damon, Inger K.
Kroon, Erna Geessien
author_role author
author2 Romero, Nadia Gallardo
Trindade, Giliane
Keckler, M. Shannon
Karem, Kevin
Carroll, Darin
Campos, Marco Antônio da Silva
Vieira, Leda Quercia
Fonseca, Flávio Guimarães da
Ferreira, Paulo César Peregrino
Bonjardim, Claudio Antonio
Damon, Inger K.
Kroon, Erna Geessien
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mota, Bruno Eduardo Fernandes
Romero, Nadia Gallardo
Trindade, Giliane
Keckler, M. Shannon
Karem, Kevin
Carroll, Darin
Campos, Marco Antônio da Silva
Vieira, Leda Quercia
Fonseca, Flávio Guimarães da
Ferreira, Paulo César Peregrino
Bonjardim, Claudio Antonio
Damon, Inger K.
Kroon, Erna Geessien
dc.subject.en.pt_BR.fl_str_mv Adaptive Immunity/immunology
CD8-Positive T-Lymphocytes/immunology
Smallpox/immunology
Vaccinia/prevention & control
topic Adaptive Immunity/immunology
CD8-Positive T-Lymphocytes/immunology
Smallpox/immunology
Vaccinia/prevention & control
description Universidade Federal de Minas Gerais. Departamento de Microbiologia. Laboratório de Virus Belo Horizonte, MG, Brazil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-11-26T17:38:30Z
dc.date.available.fl_str_mv 2014-11-26T17:38:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MOTA, Bruno Eduardo Fernandes et al. Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus. PLoS One, v. 6, n. 4, p. e18924, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8979
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0018924
identifier_str_mv MOTA, Bruno Eduardo Fernandes et al. Adverse Events Post Smallpox-Vaccination: Insights from Tail Scarification Infection in Mice with Vaccinia virus. PLoS One, v. 6, n. 4, p. e18924, 2011.
1932-6203
10.1371/journal.pone.0018924
url https://www.arca.fiocruz.br/handle/icict/8979
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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