Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2003 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7884 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
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Oliveira, Camila Indiani deBafica, Andre Luiz BarbosaOliveira, FabianoFavali, Cecilia Beatriz FiuzaCorrea, TaniaFreitas, Luiz Antonio Rodrigues deNascimento, Eliane GóesCosta, Jackson Mauricio LopesBarral, Aldina Maria Prado2014-07-09T13:53:09Z2014-07-09T13:53:09Z2003OLIVEIRA, C. I. de et al. Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. Clinical Infectious Disease, v. 37, n. 11, p. e149-153, 2003.1537-6591https://www.arca.fiocruz.br/handle/icict/7884engInfectious Diseases Society of AmericaClinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilCentro de Referência para Doenças Endêmicas Pirajá da Silva. Jequié, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilWe evaluated the use of polymerase chain reaction (PCR) for diagnosis of American cutaneous leishmaniasis (ACL) in an area in Bahia, Brazil, where Leishmania braziliensis is endemic. Leishmania DNA was detected in 50 cases, yielding a positivity rate of 100%, which was higher than the rates for all of the other diagnostic methods studied--namely, the Montenegro skin test, anti-Leishmania serological testing, and microscopic examination of lesion biopsy specimens. These findings have led us to propose guidelines for the diagnosis of ACL that use PCR as the principal means of parasitological confirmation of casesLeishmania braziliensis/isolamento & purificaçãoLeishmaniose Cutânea/diagnósticoReação em Cadeia da Polimerase/métodosAdolescenteAdultoIdosoIdoso de 80 Anos ou maisAnimaisCriançaPré-EscolarPrimers do DNADNA de Protozoário/análiseFemininoHumanosLeishmania braziliensis/genéticaMasculinoMeia-IdadeSensibilidade e Especificidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALOliveira CI Clinical utility....pdfOliveira CI Clinical utility....pdfapplication/pdf108313https://www.arca.fiocruz.br/bitstream/icict/7884/1/Oliveira%20CI%20Clinical%20utility....pdfa0806eadf613e91fe4ca5607f9f9f6c3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7884/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTOliveira CI Clinical utility....pdf.txtOliveira CI Clinical utility....pdf.txtExtracted texttext/plain22623https://www.arca.fiocruz.br/bitstream/icict/7884/3/Oliveira%20CI%20Clinical%20utility....pdf.txt03c944d216298e9b7ea5c21fa6f1bbebMD53icict/78842023-03-15 14:33:16.489oai:www.arca.fiocruz.br:icict/7884TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. |
| title |
Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. |
| spellingShingle |
Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. Oliveira, Camila Indiani de Leishmania braziliensis/isolamento & purificação Leishmaniose Cutânea/diagnóstico Reação em Cadeia da Polimerase/métodos Adolescente Adulto Idoso Idoso de 80 Anos ou mais Animais Criança Pré-Escolar Primers do DNA DNA de Protozoário/análise Feminino Humanos Leishmania braziliensis/genética Masculino Meia-Idade Sensibilidade e Especificidade |
| title_short |
Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. |
| title_full |
Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. |
| title_fullStr |
Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. |
| title_full_unstemmed |
Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. |
| title_sort |
Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. |
| author |
Oliveira, Camila Indiani de |
| author_facet |
Oliveira, Camila Indiani de Bafica, Andre Luiz Barbosa Oliveira, Fabiano Favali, Cecilia Beatriz Fiuza Correa, Tania Freitas, Luiz Antonio Rodrigues de Nascimento, Eliane Góes Costa, Jackson Mauricio Lopes Barral, Aldina Maria Prado |
| author_role |
author |
| author2 |
Bafica, Andre Luiz Barbosa Oliveira, Fabiano Favali, Cecilia Beatriz Fiuza Correa, Tania Freitas, Luiz Antonio Rodrigues de Nascimento, Eliane Góes Costa, Jackson Mauricio Lopes Barral, Aldina Maria Prado |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Camila Indiani de Bafica, Andre Luiz Barbosa Oliveira, Fabiano Favali, Cecilia Beatriz Fiuza Correa, Tania Freitas, Luiz Antonio Rodrigues de Nascimento, Eliane Góes Costa, Jackson Mauricio Lopes Barral, Aldina Maria Prado |
| dc.subject.decs.pt_BR.fl_str_mv |
Leishmania braziliensis/isolamento & purificação Leishmaniose Cutânea/diagnóstico Reação em Cadeia da Polimerase/métodos Adolescente Adulto Idoso Idoso de 80 Anos ou mais Animais Criança Pré-Escolar Primers do DNA DNA de Protozoário/análise Feminino Humanos Leishmania braziliensis/genética Masculino Meia-Idade Sensibilidade e Especificidade |
| topic |
Leishmania braziliensis/isolamento & purificação Leishmaniose Cutânea/diagnóstico Reação em Cadeia da Polimerase/métodos Adolescente Adulto Idoso Idoso de 80 Anos ou mais Animais Criança Pré-Escolar Primers do DNA DNA de Protozoário/análise Feminino Humanos Leishmania braziliensis/genética Masculino Meia-Idade Sensibilidade e Especificidade |
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Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
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2003 |
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2003 |
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2014-07-09T13:53:09Z |
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2014-07-09T13:53:09Z |
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info:eu-repo/semantics/article |
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article |
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OLIVEIRA, C. I. de et al. Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. Clinical Infectious Disease, v. 37, n. 11, p. e149-153, 2003. |
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1537-6591 |
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OLIVEIRA, C. I. de et al. Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. Clinical Infectious Disease, v. 37, n. 11, p. e149-153, 2003. 1537-6591 |
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