A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.

Detalhes bibliográficos
Autor(a) principal: Santos, Ricardo Ribeiro dos
Data de Publicação: 2001
Outros Autores: Mengele Junior, José Orivaldo, Postol, Edilberto, Soares, R. A. O, Fernandez, E. Ferreira, Soares, Milena Botelho Pereira, Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5625
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Santos, Ricardo Ribeiro dosMengele Junior, José OrivaldoPostol, EdilbertoSoares, R. A. OFernandez, E. FerreiraSoares, Milena Botelho PereiraPontes-de-Carvalho, Lain Carlos2012-10-02T20:59:07Z2012-10-02T20:59:07Z2001SANTOS, R. R. dos et al. A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi. Parasite Immunology, v. 23, n. 2, p. 93-101, feb. 2001.0141-9838https://www.arca.fiocruz.br/handle/icict/5625engA heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilTo study the role of autoreactive T cells in the pathogenesis of cardiomyopathy in Chagas' disease, we generated a cell line by repeated in vitro antigenic stimulation of purified splenic CD4+ T lymphocytes from a chronically Trypanosoma cruzi-infected mouse. Cells from this line were confirmed to be CD4+ CD8- and proliferated upon stimulation with soluble heart antigens from different animal species, as well as with T. cruzi antigen, in the presence of syngeneic feeder cells. In vitro antigen stimulation of the cell line produced a Th1 cytokine profile, with high levels of IFNgamma and IL-2 and absence of IL-4, IL-5 and IL-10. The cell line also terminated the beating of fetal heart clusters in vitro when cocultured with irradiated syngeneic normal spleen cells. In situ injection of the cell line into well established heart transplants also induced the cessation of heart beating. Finally, adoptive transfer of the cell line to heart-immunized or T. cruzi-infected BALB/c nude mice caused intense heart inflammation.T-cell lineTrypanosoma cruziAutoreactivityCarditisChagas' diseaseLinfócitos T CD4-Positivos/imunologiaDoença de Chagas/imunologiaTrypanosoma cruziAnimaisLinhagem CelularDoença CrônicaTécnicas de CoculturaModelos Animais de DoençasRejeição de EnxertoTransplante de CoraçãoInterferon gama/análiseInterleucina-2/análiseCamundongosCamundongos Endogâmicos BALB CCamundongos Endogâmicos C57BLCamundongos Endogâmicos DBACamundongos NusMiocardite/imunologiaMiocárdio/citologiaMiocárdio/imunologiaCoelhosRatosBaço/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdfRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdfapplication/pdf365996https://www.arca.fiocruz.br/bitstream/icict/5625/1/Ribeiro-Dos_Santos%20R%20A%20heart%20specific%20CD4%2b%20T-cell....pdf63005200e720e052c9d7923012979ecaMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5625/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdf.txtRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdf.txtExtracted texttext/plain34457https://www.arca.fiocruz.br/bitstream/icict/5625/5/Ribeiro-Dos_Santos%20R%20A%20heart%20specific%20CD4%2b%20T-cell....pdf.txtbed27b2b20c21f2323d871ecfcc5ab4fMD55THUMBNAILRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdf.jpgRibeiro-Dos_Santos R A heart specific CD4+ T-cell....pdf.jpgGenerated Thumbnailimage/jpeg2059https://www.arca.fiocruz.br/bitstream/icict/5625/4/Ribeiro-Dos_Santos%20R%20A%20heart%20specific%20CD4%2b%20T-cell....pdf.jpgacc1ad708572342568b64ee9d878ae34MD54icict/56252023-03-15 14:33:12.689oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:12Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
spellingShingle A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
Santos, Ricardo Ribeiro dos
T-cell line
Trypanosoma cruzi
Autoreactivity
Carditis
Chagas' disease
Linfócitos T CD4-Positivos/imunologia
Doença de Chagas/imunologia
Trypanosoma cruzi
Animais
Linhagem Celular
Doença Crônica
Técnicas de Cocultura
Modelos Animais de Doenças
Rejeição de Enxerto
Transplante de Coração
Interferon gama/análise
Interleucina-2/análise
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos DBA
Camundongos Nus
Miocardite/imunologia
Miocárdio/citologia
Miocárdio/imunologia
Coelhos
Ratos
Baço/imunologia
title_short A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title_full A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title_fullStr A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title_full_unstemmed A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
title_sort A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
author Santos, Ricardo Ribeiro dos
author_facet Santos, Ricardo Ribeiro dos
Mengele Junior, José Orivaldo
Postol, Edilberto
Soares, R. A. O
Fernandez, E. Ferreira
Soares, Milena Botelho Pereira
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Mengele Junior, José Orivaldo
Postol, Edilberto
Soares, R. A. O
Fernandez, E. Ferreira
Soares, Milena Botelho Pereira
Pontes-de-Carvalho, Lain Carlos
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Ricardo Ribeiro dos
Mengele Junior, José Orivaldo
Postol, Edilberto
Soares, R. A. O
Fernandez, E. Ferreira
Soares, Milena Botelho Pereira
Pontes-de-Carvalho, Lain Carlos
dc.subject.en.pt_BR.fl_str_mv T-cell line
Trypanosoma cruzi
Autoreactivity
Carditis
Chagas' disease
topic T-cell line
Trypanosoma cruzi
Autoreactivity
Carditis
Chagas' disease
Linfócitos T CD4-Positivos/imunologia
Doença de Chagas/imunologia
Trypanosoma cruzi
Animais
Linhagem Celular
Doença Crônica
Técnicas de Cocultura
Modelos Animais de Doenças
Rejeição de Enxerto
Transplante de Coração
Interferon gama/análise
Interleucina-2/análise
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos DBA
Camundongos Nus
Miocardite/imunologia
Miocárdio/citologia
Miocárdio/imunologia
Coelhos
Ratos
Baço/imunologia
dc.subject.decs.pt_BR.fl_str_mv Linfócitos T CD4-Positivos/imunologia
Doença de Chagas/imunologia
Trypanosoma cruzi
Animais
Linhagem Celular
Doença Crônica
Técnicas de Cocultura
Modelos Animais de Doenças
Rejeição de Enxerto
Transplante de Coração
Interferon gama/análise
Interleucina-2/análise
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos DBA
Camundongos Nus
Miocardite/imunologia
Miocárdio/citologia
Miocárdio/imunologia
Coelhos
Ratos
Baço/imunologia
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2012-10-02T20:59:07Z
dc.date.available.fl_str_mv 2012-10-02T20:59:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, R. R. dos et al. A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi. Parasite Immunology, v. 23, n. 2, p. 93-101, feb. 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5625
dc.identifier.issn.none.fl_str_mv 0141-9838
identifier_str_mv SANTOS, R. R. dos et al. A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi. Parasite Immunology, v. 23, n. 2, p. 93-101, feb. 2001.
0141-9838
url https://www.arca.fiocruz.br/handle/icict/5625
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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