Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis

Detalhes bibliográficos
Autor(a) principal: Oliveira, Manoel Marques Evangelista
Data de Publicação: 2020
Outros Autores: Muniz, Mauro de Medeiros, Almeida-Paes, Rodrigo, Zancope-Oliveira, Rosely Maria, Freitas, Andrea D'Avila, Lima, Marco A., Gutierrez-Galhardo, Maria Clara, Freitas, Dayvison Francis Saraiva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43097
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.
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spelling Oliveira, Manoel Marques EvangelistaMuniz, Mauro de MedeirosAlmeida-Paes, RodrigoZancope-Oliveira, Rosely MariaFreitas, Andrea D'AvilaLima, Marco A.Gutierrez-Galhardo, Maria ClaraFreitas, Dayvison Francis Saraiva2020-08-31T22:54:17Z2020-08-31T22:54:17Z2020OLIVEIRA, Manoel Marques Evangelista et al. Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis. PLoS Neglected Tropical Diseases, v. 14, n. 7, p. 1-4, 2020.1935-2727https://www.arca.fiocruz.br/handle/icict/4309710.1371/journal.pntd.0008196engPublic Library of ScienceCerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Serviço Médico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Cases of meningoencephalitis have increased in the zoonotic hyperendemia of sporotrichosis in the state of Rio de Janeiro, Brazil. The gold standard for the diagnosis of sporotrichosis is the isolation of Sporothrix spp. from clinical specimens, not always possible from the cerebrospinal fluid (CSF) of these patients, since fungus recovery in this specimen is difficult in most cases. This limitation led us to pursue a new approach on central nervous system (CNS) sporotrichosis diagnosis based on existing molecular methodologies for the detection of Sporothrix spp. in skin samples. Kano and colleagues (2003) designed species-specific primers for polymerase chain reaction (PCR) based on Sporothrix schenckii sensu lato chitin synthase 1 (CHS1) gene sequence and applied it in skin biopsy paraffin block. Hu and colleagues (2003) used a nested PCR in human clinical samples and samples from infected mice, with the 18S rRNA gene sequence as target. The assay was successfully used to detect S. schenckii DNA from strains from different areas of the world. However, Mendoza and colleagues compared the previously described nested PCR with conventional diagnostic methods, and the molecular methodology presented lower efficacy. Liu and colleagues, using the primer pair S2-R2 targeting the CHS1 gene in the PCR of biopsy tissue, verified positive results in 25 out of 30 cases (83.3%). The nested PCR targeting the partial sequence of the 18S rRNA gene was the best choice in terms of sensitivity due to a low fungal burden in CSF.Cerebrospinal FluidSporothrixSporotrichosisPolymerase Chain ReactionCentral Nervous SystemFungiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/43097/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALCerecrospinal_Dayvison_Freitas_etal_INI_2020.pdfCerecrospinal_Dayvison_Freitas_etal_INI_2020.pdfapplication/pdf390842https://www.arca.fiocruz.br/bitstream/icict/43097/2/Cerecrospinal_Dayvison_Freitas_etal_INI_2020.pdf9f217652557ca37eb3f6a53c6b6d2240MD52TEXTCerecrospinal_Dayvison_Freitas_etal_INI_2020.pdf.txtCerecrospinal_Dayvison_Freitas_etal_INI_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis
title Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis
spellingShingle Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis
Oliveira, Manoel Marques Evangelista
Cerebrospinal Fluid
Sporothrix
Sporotrichosis
Polymerase Chain Reaction
Central Nervous System
Fungi
title_short Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis
title_full Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis
title_fullStr Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis
title_full_unstemmed Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis
title_sort Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis
author Oliveira, Manoel Marques Evangelista
author_facet Oliveira, Manoel Marques Evangelista
Muniz, Mauro de Medeiros
Almeida-Paes, Rodrigo
Zancope-Oliveira, Rosely Maria
Freitas, Andrea D'Avila
Lima, Marco A.
Gutierrez-Galhardo, Maria Clara
Freitas, Dayvison Francis Saraiva
author_role author
author2 Muniz, Mauro de Medeiros
Almeida-Paes, Rodrigo
Zancope-Oliveira, Rosely Maria
Freitas, Andrea D'Avila
Lima, Marco A.
Gutierrez-Galhardo, Maria Clara
Freitas, Dayvison Francis Saraiva
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Manoel Marques Evangelista
Muniz, Mauro de Medeiros
Almeida-Paes, Rodrigo
Zancope-Oliveira, Rosely Maria
Freitas, Andrea D'Avila
Lima, Marco A.
Gutierrez-Galhardo, Maria Clara
Freitas, Dayvison Francis Saraiva
dc.subject.en.pt_BR.fl_str_mv Cerebrospinal Fluid
Sporothrix
Sporotrichosis
Polymerase Chain Reaction
Central Nervous System
Fungi
topic Cerebrospinal Fluid
Sporothrix
Sporotrichosis
Polymerase Chain Reaction
Central Nervous System
Fungi
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-31T22:54:17Z
dc.date.available.fl_str_mv 2020-08-31T22:54:17Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Manoel Marques Evangelista et al. Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis. PLoS Neglected Tropical Diseases, v. 14, n. 7, p. 1-4, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43097
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0008196
identifier_str_mv OLIVEIRA, Manoel Marques Evangelista et al. Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis. PLoS Neglected Tropical Diseases, v. 14, n. 7, p. 1-4, 2020.
1935-2727
10.1371/journal.pntd.0008196
url https://www.arca.fiocruz.br/handle/icict/43097
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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