Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/43097 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil. |
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Oliveira, Manoel Marques EvangelistaMuniz, Mauro de MedeirosAlmeida-Paes, RodrigoZancope-Oliveira, Rosely MariaFreitas, Andrea D'AvilaLima, Marco A.Gutierrez-Galhardo, Maria ClaraFreitas, Dayvison Francis Saraiva2020-08-31T22:54:17Z2020-08-31T22:54:17Z2020OLIVEIRA, Manoel Marques Evangelista et al. Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis. PLoS Neglected Tropical Diseases, v. 14, n. 7, p. 1-4, 2020.1935-2727https://www.arca.fiocruz.br/handle/icict/4309710.1371/journal.pntd.0008196engPublic Library of ScienceCerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Serviço Médico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Cases of meningoencephalitis have increased in the zoonotic hyperendemia of sporotrichosis in the state of Rio de Janeiro, Brazil. The gold standard for the diagnosis of sporotrichosis is the isolation of Sporothrix spp. from clinical specimens, not always possible from the cerebrospinal fluid (CSF) of these patients, since fungus recovery in this specimen is difficult in most cases. This limitation led us to pursue a new approach on central nervous system (CNS) sporotrichosis diagnosis based on existing molecular methodologies for the detection of Sporothrix spp. in skin samples. Kano and colleagues (2003) designed species-specific primers for polymerase chain reaction (PCR) based on Sporothrix schenckii sensu lato chitin synthase 1 (CHS1) gene sequence and applied it in skin biopsy paraffin block. Hu and colleagues (2003) used a nested PCR in human clinical samples and samples from infected mice, with the 18S rRNA gene sequence as target. The assay was successfully used to detect S. schenckii DNA from strains from different areas of the world. However, Mendoza and colleagues compared the previously described nested PCR with conventional diagnostic methods, and the molecular methodology presented lower efficacy. Liu and colleagues, using the primer pair S2-R2 targeting the CHS1 gene in the PCR of biopsy tissue, verified positive results in 25 out of 30 cases (83.3%). The nested PCR targeting the partial sequence of the 18S rRNA gene was the best choice in terms of sensitivity due to a low fungal burden in CSF.Cerebrospinal FluidSporothrixSporotrichosisPolymerase Chain ReactionCentral Nervous SystemFungiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/43097/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALCerecrospinal_Dayvison_Freitas_etal_INI_2020.pdfCerecrospinal_Dayvison_Freitas_etal_INI_2020.pdfapplication/pdf390842https://www.arca.fiocruz.br/bitstream/icict/43097/2/Cerecrospinal_Dayvison_Freitas_etal_INI_2020.pdf9f217652557ca37eb3f6a53c6b6d2240MD52TEXTCerecrospinal_Dayvison_Freitas_etal_INI_2020.pdf.txtCerecrospinal_Dayvison_Freitas_etal_INI_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis |
title |
Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis |
spellingShingle |
Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis Oliveira, Manoel Marques Evangelista Cerebrospinal Fluid Sporothrix Sporotrichosis Polymerase Chain Reaction Central Nervous System Fungi |
title_short |
Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis |
title_full |
Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis |
title_fullStr |
Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis |
title_full_unstemmed |
Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis |
title_sort |
Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis |
author |
Oliveira, Manoel Marques Evangelista |
author_facet |
Oliveira, Manoel Marques Evangelista Muniz, Mauro de Medeiros Almeida-Paes, Rodrigo Zancope-Oliveira, Rosely Maria Freitas, Andrea D'Avila Lima, Marco A. Gutierrez-Galhardo, Maria Clara Freitas, Dayvison Francis Saraiva |
author_role |
author |
author2 |
Muniz, Mauro de Medeiros Almeida-Paes, Rodrigo Zancope-Oliveira, Rosely Maria Freitas, Andrea D'Avila Lima, Marco A. Gutierrez-Galhardo, Maria Clara Freitas, Dayvison Francis Saraiva |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Manoel Marques Evangelista Muniz, Mauro de Medeiros Almeida-Paes, Rodrigo Zancope-Oliveira, Rosely Maria Freitas, Andrea D'Avila Lima, Marco A. Gutierrez-Galhardo, Maria Clara Freitas, Dayvison Francis Saraiva |
dc.subject.en.pt_BR.fl_str_mv |
Cerebrospinal Fluid Sporothrix Sporotrichosis Polymerase Chain Reaction Central Nervous System Fungi |
topic |
Cerebrospinal Fluid Sporothrix Sporotrichosis Polymerase Chain Reaction Central Nervous System Fungi |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-08-31T22:54:17Z |
dc.date.available.fl_str_mv |
2020-08-31T22:54:17Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Manoel Marques Evangelista et al. Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis. PLoS Neglected Tropical Diseases, v. 14, n. 7, p. 1-4, 2020. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/43097 |
dc.identifier.issn.pt_BR.fl_str_mv |
1935-2727 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0008196 |
identifier_str_mv |
OLIVEIRA, Manoel Marques Evangelista et al. Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis. PLoS Neglected Tropical Diseases, v. 14, n. 7, p. 1-4, 2020. 1935-2727 10.1371/journal.pntd.0008196 |
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https://www.arca.fiocruz.br/handle/icict/43097 |
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eng |
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eng |
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Public Library of Science |
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Public Library of Science |
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