A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni

Detalhes bibliográficos
Autor(a) principal: Mourão, Marina de Moraes
Data de Publicação: 2013
Outros Autores: Bitar, Mainá, Lobo, Francisco Pereira, Peconick, Ana Paula, Grynberg, Priscila, Prosdocimi, Francisco, Waisberg, Michael, Cerqueira, Gustavo Coutinho, Macedo, Andréa Mara, Machado, Carlos Renato, Yoshino, Timothy, Franco, Glória Regina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59466
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil.
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spelling Mourão, Marina de MoraesBitar, MaináLobo, Francisco PereiraPeconick, Ana PaulaGrynberg, PriscilaProsdocimi, FranciscoWaisberg, MichaelCerqueira, Gustavo CoutinhoMacedo, Andréa MaraMachado, Carlos RenatoYoshino, TimothyFranco, Glória Regina2023-07-07T14:25:31Z2023-07-07T14:25:31Z2013MOURÃO, Marina de Moraes et al. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013.0074-0276https://www.arca.fiocruz.br/handle/icict/5946610.1590/0074-0276108062013006engInstituto Oswaldo CruzA directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil.Embrapa Informática Aplicada. Laboratório de Bioinformática Aplicada. Campinas, SP, Brasil.Universidade Federal de Lavras. Setor de Medicina Veterinária Preventiva. Lavras, MG, Brasil.Universidade Federal de Minas Gerais. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Department of Pathology. University of Virginia. Charlottesville, VA, USA.Broad Institute of Massachusetts. Institute of Technology and Harvard. Cambridge, MA, USA.Universidade Federal de Minas Gerais. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil.Department of Pathobiological Sciences. University of Wisconsin. Madison, WI, USA.Universidade Federal de Minas Gerais. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brasil.Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula.Spliced leaderTrans-splicingRNA interferenceSchistosoma mansoniinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL0074-0276-mioc-108-06-707.pdf0074-0276-mioc-108-06-707.pdfapplication/pdf1108242https://www.arca.fiocruz.br/bitstream/icict/59466/2/0074-0276-mioc-108-06-707.pdfeb6a36bd2725717d8e10537d3db39073MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59466/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/594662023-07-07 11:34:28.334oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-07T14:34:28Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
spellingShingle A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
Mourão, Marina de Moraes
Spliced leader
Trans-splicing
RNA interference
Schistosoma mansoni
title_short A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title_full A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title_fullStr A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title_full_unstemmed A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title_sort A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
author Mourão, Marina de Moraes
author_facet Mourão, Marina de Moraes
Bitar, Mainá
Lobo, Francisco Pereira
Peconick, Ana Paula
Grynberg, Priscila
Prosdocimi, Francisco
Waisberg, Michael
Cerqueira, Gustavo Coutinho
Macedo, Andréa Mara
Machado, Carlos Renato
Yoshino, Timothy
Franco, Glória Regina
author_role author
author2 Bitar, Mainá
Lobo, Francisco Pereira
Peconick, Ana Paula
Grynberg, Priscila
Prosdocimi, Francisco
Waisberg, Michael
Cerqueira, Gustavo Coutinho
Macedo, Andréa Mara
Machado, Carlos Renato
Yoshino, Timothy
Franco, Glória Regina
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mourão, Marina de Moraes
Bitar, Mainá
Lobo, Francisco Pereira
Peconick, Ana Paula
Grynberg, Priscila
Prosdocimi, Francisco
Waisberg, Michael
Cerqueira, Gustavo Coutinho
Macedo, Andréa Mara
Machado, Carlos Renato
Yoshino, Timothy
Franco, Glória Regina
dc.subject.en.en_US.fl_str_mv Spliced leader
Trans-splicing
RNA interference
Schistosoma mansoni
topic Spliced leader
Trans-splicing
RNA interference
Schistosoma mansoni
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2023-07-07T14:25:31Z
dc.date.available.fl_str_mv 2023-07-07T14:25:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MOURÃO, Marina de Moraes et al. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59466
dc.identifier.issn.en_US.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-0276108062013006
identifier_str_mv MOURÃO, Marina de Moraes et al. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013.
0074-0276
10.1590/0074-0276108062013006
url https://www.arca.fiocruz.br/handle/icict/59466
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
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