Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor

Drummond, Marcela Gonçalves; Silva, Carlos Eduardo Calzavara; D’Astolfo, Diego S.; Cardoso, Fernanda Caldas; Rajão, Matheus Andrade; Mourão, Marina de Moraes; Gava, Elisandra; Oliveira, Sergio Costa; Macedo, Andrea Mara; Machado, Carlos Renato; Pena, Sergio Danilo Junho; Kitten, Gregory Thomas; Franco, Gloria Regina

Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor

Detalhes bibliográficos
Autor(a) principal:	Drummond, Marcela Gonçalves
Data de Publicação:	2009
Outros Autores:	Silva, Carlos Eduardo Calzavara, D’Astolfo, Diego S., Cardoso, Fernanda Caldas, Rajão, Matheus Andrade, Mourão, Marina de Moraes, Gava, Elisandra, Oliveira, Sergio Costa, Macedo, Andrea Mara, Machado, Carlos Renato, Pena, Sergio Danilo Junho, Kitten, Gregory Thomas, Franco, Gloria Regina
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/58819
Resumo:	Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte,MG, Brazil

Metadados do item

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network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Drummond, Marcela GonçalvesSilva, Carlos Eduardo CalzavaraD’Astolfo, Diego S.Cardoso, Fernanda CaldasRajão, Matheus AndradeMourão, Marina de MoraesGava, ElisandraOliveira, Sergio CostaMacedo, Andrea MaraMachado, Carlos RenatoPena, Sergio Danilo JunhoKitten, Gregory ThomasFranco, Gloria Regina2023-05-30T18:33:59Z2023-05-30T18:33:59Z2009DRUMMOND, Marcela Gonçalves et al. Molecular characterization of the Schistosoma mansoni zinc finger protein SmZF1 as a transcription fator. PLoS Negl Trop Dis., v. 3, n. 11, e547, 2009. doi: 10.1371/journal.pntd.0000547.1935-2735https://www.arca.fiocruz.br/handle/icict/5881910.1371/journal.pntd.0000547engPublic Library of ScienceMolecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte,MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte,MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil,Centro de Investigaciones en Bioquımica Clınica e Inmunologıa. Departamento de Bioquımica Clınica. Facultad de Ciencias Quımicas. Universidad Nacional de Cordoba. Cordoba, ArgentinaUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Imunologia de Doencas Infecciosas. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Imunologia de Doencas Infecciosas. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte,MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte,MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Laboratorio do Desenvolvimento do Coracão e Matriz Extracelular. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte, MG, BrazilBackground: During its development, the parasite Schistosoma mansoni is exposed to different environments and undergoes many morphological and physiological transformations as a result of profound changes in gene expression. Characterization of proteins involved in the regulation of these processes is of importance for the understanding of schistosome biology. Proteins containing zinc finger motifs usually participate in regulatory processes and are considered the major class of transcription factors in eukaryotes. It has already been shown, by EMSA (Eletrophoretic Mobility Shift Assay), that SmZF1, a S. mansoni zinc finger (ZF) protein, specifically binds both DNA and RNA oligonucleotides. This suggests that this protein might act as a transcription factor in the parasite. Methodology/Principal Findings: In this study we extended the characterization of SmZF1 by determining its subcellular localization and by verifying its ability to regulate gene transcription. We performed immunohistochemistry assays using adult male and female worms, cercariae and schistosomula to analyze the distribution pattern of SmZF1 and verified that the protein is mainly detected in the cells nuclei of all tested life cycle stages except for adult female worms. Also, SmZF1 was heterologously expressed in mammalian COS-7 cells to produce the recombinant protein YFP-SmZF1, which was mainly detected in the nucleus of the cells by confocal microscopy and Western blot assays. To evaluate the ability of this protein to regulate gene transcription, cells expressing YFP-SmZF1 were tested in a luciferase reporter system. In this system, the luciferase gene is downstream of a minimal promoter, upstream of which a DNA region containing four copies of the SmZF1 putative best binding site (D1-3DNA) was inserted. SmZF1 increased the reporter gene transcription by two fold (p <= 0.003) only when its specific binding site was present. Conclusion: Taken together, these results strongly support the hypothesis that SmZF1 acts as a transcription factor in S. mansoniinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALpntd.0000547 1..10.pdfpntd.0000547 1..10.pdfapplication/pdf343917https://www.arca.fiocruz.br/bitstream/icict/58819/2/pntd.0000547%201..10.pdf785ec2177198314efc092cd011075673MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58819/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/588192023-05-30 15:34:01.254oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-30T18:34:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv	Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor
title	Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor
spellingShingle	Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor Drummond, Marcela Gonçalves
title_short	Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor
title_full	Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor
title_fullStr	Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor
title_full_unstemmed	Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor
title_sort	Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor
author	Drummond, Marcela Gonçalves
author_facet	Drummond, Marcela Gonçalves Silva, Carlos Eduardo Calzavara D’Astolfo, Diego S. Cardoso, Fernanda Caldas Rajão, Matheus Andrade Mourão, Marina de Moraes Gava, Elisandra Oliveira, Sergio Costa Macedo, Andrea Mara Machado, Carlos Renato Pena, Sergio Danilo Junho Kitten, Gregory Thomas Franco, Gloria Regina
author_role	author
author2	Silva, Carlos Eduardo Calzavara D’Astolfo, Diego S. Cardoso, Fernanda Caldas Rajão, Matheus Andrade Mourão, Marina de Moraes Gava, Elisandra Oliveira, Sergio Costa Macedo, Andrea Mara Machado, Carlos Renato Pena, Sergio Danilo Junho Kitten, Gregory Thomas Franco, Gloria Regina
author2_role	author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Drummond, Marcela Gonçalves Silva, Carlos Eduardo Calzavara D’Astolfo, Diego S. Cardoso, Fernanda Caldas Rajão, Matheus Andrade Mourão, Marina de Moraes Gava, Elisandra Oliveira, Sergio Costa Macedo, Andrea Mara Machado, Carlos Renato Pena, Sergio Danilo Junho Kitten, Gregory Thomas Franco, Gloria Regina
description	Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Genetica Bioquımica. Belo Horizonte,MG, Brazil
publishDate	2009
dc.date.issued.fl_str_mv	2009
dc.date.accessioned.fl_str_mv	2023-05-30T18:33:59Z
dc.date.available.fl_str_mv	2023-05-30T18:33:59Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	DRUMMOND, Marcela Gonçalves et al. Molecular characterization of the Schistosoma mansoni zinc finger protein SmZF1 as a transcription fator. PLoS Negl Trop Dis., v. 3, n. 11, e547, 2009. doi: 10.1371/journal.pntd.0000547.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/58819
dc.identifier.issn.en_US.fl_str_mv	1935-2735
dc.identifier.doi.none.fl_str_mv	10.1371/journal.pntd.0000547
identifier_str_mv	DRUMMOND, Marcela Gonçalves et al. Molecular characterization of the Schistosoma mansoni zinc finger protein SmZF1 as a transcription fator. PLoS Negl Trop Dis., v. 3, n. 11, e547, 2009. doi: 10.1371/journal.pntd.0000547. 1935-2735 10.1371/journal.pntd.0000547
url	https://www.arca.fiocruz.br/handle/icict/58819
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Public Library of Science
publisher.none.fl_str_mv	Public Library of Science
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/58819/2/pntd.0000547%201..10.pdf https://www.arca.fiocruz.br/bitstream/icict/58819/1/license.txt
bitstream.checksum.fl_str_mv	785ec2177198314efc092cd011075673 8a4605be74aa9ea9d79846c1fba20a33
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
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Molecular Characterization of the Schistosoma mansoni Zinc Finger Protein SmZF1 as a Transcription Factor

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