A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/2745 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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Teixeira, Márcia Cristina AquinoOliveira, Geraldo Gileno de SáSilvany, Marco Antonio AraujoNeves, Neuza Maria AlcântaraSoares, Milena Botelho PereiraSantos, Ricardo Ribeiro dosJerônimo, Selma Maria BezerraCosta, Carlos Henrique NerydosSantos, Washington Luis ConradoEichinger, DanielPontes-de-Carvalho, Lain Carlos2011-07-04T16:24:39Z2011-07-04T16:24:39Z2006TEIXEIRA, M. C. A. et al. A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries. Biologicals, v.35, n.1, p.51-54, 2007.1045-1056https://www.arca.fiocruz.br/handle/icict/274510.1016/j.biologicals.2006.01.005engAntígeno RecombinanteBiblioteca de cDNASorodiagnósticoSensibilidade ImunoensaioLeishmania InfantumLeishmaniose VisceralRecombinant AntigencDNA LibrarySerodiagnosisImmunoassay SensitivityLeishmania InfantumVisceral LeishmaniasisBiblioteca GênicaTestes SorológicosImunoensaioLeishmania InfantumLeishmaniose VisceralA strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic librariesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFaculdade de Tecnologia e Ciências - FTC. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFaculdade de Tecnologia e Ciências - FTC. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Rio Grande do Norte. Natal, RN, BrasilUniversidade Federal do Piauí. Teresina. Piauí, PI, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilNew York University Medical Center. New York, NI, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilDifferent individuals, when infected with the same parasite, rarely produce antibodies against the same set of antigens. Indeed, unless a particular antigen happens to be recognized by antibodies in all individuals, the use of a single antigen in the serodiagnosis of parasitic diseases leads, invariably, to false-negative results. A straightforward method for pin-pointing, in genetic libraries, the precise antigens that would increase serodiagnostic assay sensitivities is presented. The method is based on the utilization of sera that produced false-negative results against previously available antigens. Employing this false-negative serum-selection methodology for the identification of new Leishmania infantum recombinant antigens (rAgs), the sensitivity of a dipstick assay for anti-Leishmania antibodies in a panel of sera from patients with visceral leishmaniasis was increased from 83.3% to 98.1%, without affecting its specificity, by the inclusion of a fifth and a sixth L. infantum rAg.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALTeixeira_Oliveira_Silvany_etal.pdfTeixeira_Oliveira_Silvany_etal.pdfapplication/pdf386287https://www.arca.fiocruz.br/bitstream/icict/2745/1/Teixeira_Oliveira_Silvany_etal.pdfaa50d6f913a6dafb47b4a4763f491bd8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2745/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTTeixeira_Oliveira_Silvany_etal.pdf.txtTeixeira_Oliveira_Silvany_etal.pdf.txtExtracted texttext/plain19687https://www.arca.fiocruz.br/bitstream/icict/2745/5/Teixeira_Oliveira_Silvany_etal.pdf.txt93ef2f313eb7a92818e17a39329d008eMD55THUMBNAILTeixeira_Oliveira_Silvany_etal.pdf.jpgTeixeira_Oliveira_Silvany_etal.pdf.jpgGenerated Thumbnailimage/jpeg2159https://www.arca.fiocruz.br/bitstream/icict/2745/4/Teixeira_Oliveira_Silvany_etal.pdf.jpge6298215e654224eada7cba15c972d84MD54icict/27452023-03-15 14:33:19.817oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries |
| title |
A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries |
| spellingShingle |
A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries Teixeira, Márcia Cristina Aquino Antígeno Recombinante Biblioteca de cDNA Sorodiagnóstico Sensibilidade Imunoensaio Leishmania Infantum Leishmaniose Visceral Recombinant Antigen cDNA Library Serodiagnosis Immunoassay Sensitivity Leishmania Infantum Visceral Leishmaniasis Biblioteca Gênica Testes Sorológicos Imunoensaio Leishmania Infantum Leishmaniose Visceral |
| title_short |
A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries |
| title_full |
A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries |
| title_fullStr |
A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries |
| title_full_unstemmed |
A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries |
| title_sort |
A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries |
| author |
Teixeira, Márcia Cristina Aquino |
| author_facet |
Teixeira, Márcia Cristina Aquino Oliveira, Geraldo Gileno de Sá Silvany, Marco Antonio Araujo Neves, Neuza Maria Alcântara Soares, Milena Botelho Pereira Santos, Ricardo Ribeiro dos Jerônimo, Selma Maria Bezerra Costa, Carlos Henrique Nery dosSantos, Washington Luis Conrado Eichinger, Daniel Pontes-de-Carvalho, Lain Carlos |
| author_role |
author |
| author2 |
Oliveira, Geraldo Gileno de Sá Silvany, Marco Antonio Araujo Neves, Neuza Maria Alcântara Soares, Milena Botelho Pereira Santos, Ricardo Ribeiro dos Jerônimo, Selma Maria Bezerra Costa, Carlos Henrique Nery dosSantos, Washington Luis Conrado Eichinger, Daniel Pontes-de-Carvalho, Lain Carlos |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Teixeira, Márcia Cristina Aquino Oliveira, Geraldo Gileno de Sá Silvany, Marco Antonio Araujo Neves, Neuza Maria Alcântara Soares, Milena Botelho Pereira Santos, Ricardo Ribeiro dos Jerônimo, Selma Maria Bezerra Costa, Carlos Henrique Nery dosSantos, Washington Luis Conrado Eichinger, Daniel Pontes-de-Carvalho, Lain Carlos |
| dc.subject.other.pt_BR.fl_str_mv |
Antígeno Recombinante Biblioteca de cDNA Sorodiagnóstico Sensibilidade Imunoensaio Leishmania Infantum Leishmaniose Visceral |
| topic |
Antígeno Recombinante Biblioteca de cDNA Sorodiagnóstico Sensibilidade Imunoensaio Leishmania Infantum Leishmaniose Visceral Recombinant Antigen cDNA Library Serodiagnosis Immunoassay Sensitivity Leishmania Infantum Visceral Leishmaniasis Biblioteca Gênica Testes Sorológicos Imunoensaio Leishmania Infantum Leishmaniose Visceral |
| dc.subject.en.pt_BR.fl_str_mv |
Recombinant Antigen cDNA Library Serodiagnosis Immunoassay Sensitivity Leishmania Infantum Visceral Leishmaniasis |
| dc.subject.decs.pt_BR.fl_str_mv |
Biblioteca Gênica Testes Sorológicos Imunoensaio Leishmania Infantum Leishmaniose Visceral |
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Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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2006 |
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2006 |
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2011-07-04T16:24:39Z |
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2011-07-04T16:24:39Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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TEIXEIRA, M. C. A. et al. A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries. Biologicals, v.35, n.1, p.51-54, 2007. |
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1045-1056 |
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10.1016/j.biologicals.2006.01.005 |
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TEIXEIRA, M. C. A. et al. A strategy for identifying serodiagnostically relevant antigens of Leishmania or other pathogens in genetic libraries. Biologicals, v.35, n.1, p.51-54, 2007. 1045-1056 10.1016/j.biologicals.2006.01.005 |
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