Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus

Detalhes bibliográficos
Autor(a) principal: Vieira, Debora Ferreira Barreto
Data de Publicação: 2017
Outros Autores: Jácome, Fernanda Cunha, Silva, Marcos Alexandre Nunes da, Caldas, Gabriela Cardoso, Filippis, Ana Maria Bispo de, Sequeira, Patrícia Carvalho de, Souza, Elen Mello de, Andrade, Audrien Alves, Manso, Pedro Paulo de Abreu, Trindade, Gisela Freitas, Lima, Sheila Maria Barbosa, Barth, Ortrud Monika
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23162
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
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spelling Vieira, Debora Ferreira BarretoJácome, Fernanda CunhaSilva, Marcos Alexandre Nunes daCaldas, Gabriela CardosoFilippis, Ana Maria Bispo deSequeira, Patrícia Carvalho deSouza, Elen Mello deAndrade, Audrien AlvesManso, Pedro Paulo de AbreuTrindade, Gisela FreitasLima, Sheila Maria BarbosaBarth, Ortrud Monika2017-11-14T13:12:48Z2017-11-14T13:12:48Z2017VIEIRA, Debora Ferreira Barreto; et al. Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus. Plos One, v.12, n.9, e0184397, 18p, Sept. 2107.1932-6203https://www.arca.fiocruz.br/handle/icict/2316210.1371/journal.pone.0184397engPublic Library of ScienceZika VirusBrasilCélulas VeroPesquisaZika virusBrazilVero cellsC6/36 cellsstructural investigationStructural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil .Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.Zika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) and African green monkey kidney epithelial cells (Vero cells) were inoculated with a ZIKV sample isolated from a Brazilian patient, and the infection was characterized by immunofluorescence staining, phase contrast light microscopy, transmission electron microscopy and real-time RT-PCR. The infection was observed in both cell lineages, and ZIKV particles were observed inside lysosomes, the rough endoplasmic reticulum and viroplasm-like structures. The susceptibility of C6/36 and Vero cells to ZIKV infection was demonstrated. Moreover, this study showed that part of the replicative cycle may occur within viroplasm-like structures, which has not been previously demonstrated in other flaviviruses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83142https://www.arca.fiocruz.br/bitstream/icict/23162/1/license.txt37a74d92f1f1fd437ab11671160e0230MD51ORIGINALve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017 .pdfve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017 .pdfapplication/pdf32395268https://www.arca.fiocruz.br/bitstream/icict/23162/2/ve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017%20.pdfa29aeece74c9bb979c75face28995a5cMD52TEXTve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017 .pdf.txtve_Barreto-Vieira_Debora_Ferreira_etal_IOC_2017 .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
spellingShingle Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
Vieira, Debora Ferreira Barreto
Zika Virus
Brasil
Células Vero
Pesquisa
Zika virus
Brazil
Vero cells
C6/36 cells
structural investigation
title_short Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title_full Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title_fullStr Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title_full_unstemmed Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
title_sort Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
author Vieira, Debora Ferreira Barreto
author_facet Vieira, Debora Ferreira Barreto
Jácome, Fernanda Cunha
Silva, Marcos Alexandre Nunes da
Caldas, Gabriela Cardoso
Filippis, Ana Maria Bispo de
Sequeira, Patrícia Carvalho de
Souza, Elen Mello de
Andrade, Audrien Alves
Manso, Pedro Paulo de Abreu
Trindade, Gisela Freitas
Lima, Sheila Maria Barbosa
Barth, Ortrud Monika
author_role author
author2 Jácome, Fernanda Cunha
Silva, Marcos Alexandre Nunes da
Caldas, Gabriela Cardoso
Filippis, Ana Maria Bispo de
Sequeira, Patrícia Carvalho de
Souza, Elen Mello de
Andrade, Audrien Alves
Manso, Pedro Paulo de Abreu
Trindade, Gisela Freitas
Lima, Sheila Maria Barbosa
Barth, Ortrud Monika
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Debora Ferreira Barreto
Jácome, Fernanda Cunha
Silva, Marcos Alexandre Nunes da
Caldas, Gabriela Cardoso
Filippis, Ana Maria Bispo de
Sequeira, Patrícia Carvalho de
Souza, Elen Mello de
Andrade, Audrien Alves
Manso, Pedro Paulo de Abreu
Trindade, Gisela Freitas
Lima, Sheila Maria Barbosa
Barth, Ortrud Monika
dc.subject.other.pt_BR.fl_str_mv Zika Virus
Brasil
Células Vero
Pesquisa
topic Zika Virus
Brasil
Células Vero
Pesquisa
Zika virus
Brazil
Vero cells
C6/36 cells
structural investigation
dc.subject.en.pt_BR.fl_str_mv Zika virus
Brazil
Vero cells
C6/36 cells
structural investigation
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-11-14T13:12:48Z
dc.date.available.fl_str_mv 2017-11-14T13:12:48Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIEIRA, Debora Ferreira Barreto; et al. Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus. Plos One, v.12, n.9, e0184397, 18p, Sept. 2107.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23162
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0184397
identifier_str_mv VIEIRA, Debora Ferreira Barreto; et al. Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus. Plos One, v.12, n.9, e0184397, 18p, Sept. 2107.
1932-6203
10.1371/journal.pone.0184397
url https://www.arca.fiocruz.br/handle/icict/23162
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/23162/1/license.txt
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