Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer

Detalhes bibliográficos
Autor(a) principal: Fischer, Juliana de Saldanha da Gama
Data de Publicação: 2014
Outros Autores: Carvalho, Paulo Costa, Canedo, Nathalie H. S., Gonçalves, Katia Maria da S., Aquino, Priscila Ferreira de, Almeida, Barbara, Pannain, Vera L. N., Carvalho, Maria da Gloria da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16043
Resumo: CNPq, FAPERJ, Fundação do Câncer
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spelling Fischer, Juliana de Saldanha da GamaCarvalho, Paulo CostaCanedo, Nathalie H. S.Gonçalves, Katia Maria da S.Aquino, Priscila Ferreira deAlmeida, BarbaraPannain, Vera L. N.Carvalho, Maria da Gloria da Costa2016-10-03T13:54:57Z2016-10-03T13:54:57Z2014FISCHER, Juliana de Saldanha da Gama et al. Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer. Austin Proteomics, v. 1, n. 1, p. 1-7, 2014.2471-0164https://www.arca.fiocruz.br/handle/icict/16043CNPq, FAPERJ, Fundação do CâncerFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Among the many challenges in identifying novel tumoral diagnostic, prognostic, and personal therapy biomarkers we highlight the challenge of dealing with a single tumor’s molecular complexity and heterogeneity. Formalin-fixed paraffin-embedded (FFPE) tissue specimens are commonly used by pathologists for diagnosing cancer; this material comprises a valuable resource for molecular studies addressing tumoral molecular profiles. Here we demonstrate the effectiveness of shotgun proteomics in assessing the cellular heterogeneity using FFPE tissues slides under three different scenarios: 1) Comparing astrocytoma grade I versus glioblastoma, 2) Assessing different morphological areas from the same histological slide of a glioblastoma and comparing the identifications with those from a fresh tissue, 3) Comparing two different histopathological profiles in the same patient’s liver: cirrhosis and cancer. Briefly, contents of FFPE slides were scraped, washed three times with xylene and then rehydrated in a grade ethanol series [100%, 90%(v/v), 70 % (v/v)]. A total of 100 µL of 0.2% (w/v) RapiGestTM in 50mM ammonium bicarbonate was then added to each sample. Tryptic digests were separated by reversed-phase capillary liquid chromatography coupled to nano-electrospray high-resolution mass spectrometry for identification. The samples were analyzed in technical replicates by LC-MS/MS on an Orbitrap XL. Our datasets enabled elucidating key tumoral regions allowing a better comprehension of such specific tumor areas; the identifications and raw data are available at http://max.ioc.fiocruz.br/julif/ffpecases.engAustin Publishing GroupGlioblastomaFormaldehydeGlioblastomaLiver NeoplasmsCirrhosisShotgun proteomicsFormaldehídoNeoplasias HepáticasFormaldeídoNeoplasias HepáticasApplications of formalin fixed paraffin-embedded tissue proteomics in the study of cancerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/16043/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALApplications of Formalin Fixed Paraffin.pdfApplications of Formalin Fixed 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dc.title.pt_BR.fl_str_mv Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer
title Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer
spellingShingle Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer
Fischer, Juliana de Saldanha da Gama
Glioblastoma
Formaldehyde
Glioblastoma
Liver Neoplasms
Cirrhosis
Shotgun proteomics
Formaldehído
Neoplasias Hepáticas
Formaldeído
Neoplasias Hepáticas
title_short Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer
title_full Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer
title_fullStr Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer
title_full_unstemmed Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer
title_sort Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer
author Fischer, Juliana de Saldanha da Gama
author_facet Fischer, Juliana de Saldanha da Gama
Carvalho, Paulo Costa
Canedo, Nathalie H. S.
Gonçalves, Katia Maria da S.
Aquino, Priscila Ferreira de
Almeida, Barbara
Pannain, Vera L. N.
Carvalho, Maria da Gloria da Costa
author_role author
author2 Carvalho, Paulo Costa
Canedo, Nathalie H. S.
Gonçalves, Katia Maria da S.
Aquino, Priscila Ferreira de
Almeida, Barbara
Pannain, Vera L. N.
Carvalho, Maria da Gloria da Costa
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fischer, Juliana de Saldanha da Gama
Carvalho, Paulo Costa
Canedo, Nathalie H. S.
Gonçalves, Katia Maria da S.
Aquino, Priscila Ferreira de
Almeida, Barbara
Pannain, Vera L. N.
Carvalho, Maria da Gloria da Costa
dc.subject.other.pt_BR.fl_str_mv Glioblastoma
topic Glioblastoma
Formaldehyde
Glioblastoma
Liver Neoplasms
Cirrhosis
Shotgun proteomics
Formaldehído
Neoplasias Hepáticas
Formaldeído
Neoplasias Hepáticas
dc.subject.en.pt_BR.fl_str_mv Formaldehyde
Glioblastoma
Liver Neoplasms
Cirrhosis
Shotgun proteomics
dc.subject.es.es.fl_str_mv Formaldehído
Neoplasias Hepáticas
dc.subject.decs.pt_BR.fl_str_mv Formaldeído
Neoplasias Hepáticas
description CNPq, FAPERJ, Fundação do Câncer
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2016-10-03T13:54:57Z
dc.date.available.fl_str_mv 2016-10-03T13:54:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FISCHER, Juliana de Saldanha da Gama et al. Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer. Austin Proteomics, v. 1, n. 1, p. 1-7, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16043
dc.identifier.issn.pt_BR.fl_str_mv 2471-0164
identifier_str_mv FISCHER, Juliana de Saldanha da Gama et al. Applications of formalin fixed paraffin-embedded tissue proteomics in the study of cancer. Austin Proteomics, v. 1, n. 1, p. 1-7, 2014.
2471-0164
url https://www.arca.fiocruz.br/handle/icict/16043
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Austin Publishing Group
publisher.none.fl_str_mv Austin Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/16043/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/16043/2/Applications%20of%20Formalin%20Fixed%20Paraffin.pdf
https://www.arca.fiocruz.br/bitstream/icict/16043/3/Applications%20of%20Formalin%20Fixed%20Paraffin.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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