Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/32656 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. |
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Santos, Fabiana Cristina FulcoLira, Laís Ariane de SiqueiraMontenegro, Rosana de AlbuquerqueLima, Juliana Figueirêdo da CostaLima, Andrea SantosSchindler, Haiana CharifkerMontenegro, Lílian Maria Lapa2019-04-22T13:30:48Z2019-04-22T13:30:48Z2018SANTOS, F. C. F. et al. Performance of the IS6110-TaqMan® Assay in the Diagnosis of Extrapulmonary Tuberculosis from Different Biological Samples. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 3, p. 331-337, jun. 2018.1678-9849https://www.arca.fiocruz.br/handle/icict/3265610.1590/0037-8682-0372-2017engPerformance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Introduction: This study evaluated the performance of the IS6110-TaqMan® assay in different types of biological samples and tissues for laboratory diagnosis of extrapulmonary tuberculosis. Methods: 143 biological samples and tissues from patients with suspected extrapulmonary tuberculosis from the health services of Recife/Pernambuco/Brazil were evaluated with the IS6110-TaqMan® assay. Results: The sensitivities of the IS6110-TaqMan® assay calculated for blood, urine, both blood and urine samples, tissue biopsies, extrapulmonary body fluid samples, and all samples from patients calculated together were 55.9%, 33.3%, 68.8%, 43.8%, 29.6%, and 73.7%, respectively, and the specificities were 80%, 100%, 78.6%, 100%, 100%, and 84.2%, respectively. Conclusions: The accuracy of qPCR was high in various clinical sample types. The analysis of more than one type of clinical sample collected from the same patient with extrapulmonary tuberculosis enhances the diagnostic power of the IS6110-TaqMan® assay when compared with the use of only one clinical sample.Mycobacterium tuberculosis complexIS6110-TaqMan® assayMolecular diagnosisExtrapulmonary tuberculosisDNA bacteriano / análiseDNA Bacteriano / genéticaDNA bacteriano / isolamento e purificaçãoMétodo Duplo-cegoHumanosMycobacterium tuberculosis / genéticaMycobacterium tuberculosis / isolamento & purificaçãoEstudos prospectivosReação em Cadeia da Polimerase em Tempo RealReprodutibilidade dos resultadosSensibilidade e EspecificidadeTuberculose / diagnósticoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples |
| title |
Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples |
| spellingShingle |
Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples Santos, Fabiana Cristina Fulco Mycobacterium tuberculosis complex IS6110-TaqMan® assay Molecular diagnosis Extrapulmonary tuberculosis DNA bacteriano / análise DNA Bacteriano / genética DNA bacteriano / isolamento e purificação Método Duplo-cego Humanos Mycobacterium tuberculosis / genética Mycobacterium tuberculosis / isolamento & purificação Estudos prospectivos Reação em Cadeia da Polimerase em Tempo Real Reprodutibilidade dos resultados Sensibilidade e Especificidade Tuberculose / diagnóstico |
| title_short |
Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples |
| title_full |
Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples |
| title_fullStr |
Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples |
| title_full_unstemmed |
Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples |
| title_sort |
Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples |
| author |
Santos, Fabiana Cristina Fulco |
| author_facet |
Santos, Fabiana Cristina Fulco Lira, Laís Ariane de Siqueira Montenegro, Rosana de Albuquerque Lima, Juliana Figueirêdo da Costa Lima, Andrea Santos Schindler, Haiana Charifker Montenegro, Lílian Maria Lapa |
| author_role |
author |
| author2 |
Lira, Laís Ariane de Siqueira Montenegro, Rosana de Albuquerque Lima, Juliana Figueirêdo da Costa Lima, Andrea Santos Schindler, Haiana Charifker Montenegro, Lílian Maria Lapa |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Fabiana Cristina Fulco Lira, Laís Ariane de Siqueira Montenegro, Rosana de Albuquerque Lima, Juliana Figueirêdo da Costa Lima, Andrea Santos Schindler, Haiana Charifker Montenegro, Lílian Maria Lapa |
| dc.subject.en.pt_BR.fl_str_mv |
Mycobacterium tuberculosis complex IS6110-TaqMan® assay Molecular diagnosis Extrapulmonary tuberculosis |
| topic |
Mycobacterium tuberculosis complex IS6110-TaqMan® assay Molecular diagnosis Extrapulmonary tuberculosis DNA bacteriano / análise DNA Bacteriano / genética DNA bacteriano / isolamento e purificação Método Duplo-cego Humanos Mycobacterium tuberculosis / genética Mycobacterium tuberculosis / isolamento & purificação Estudos prospectivos Reação em Cadeia da Polimerase em Tempo Real Reprodutibilidade dos resultados Sensibilidade e Especificidade Tuberculose / diagnóstico |
| dc.subject.decs.pt_BR.fl_str_mv |
DNA bacteriano / análise DNA Bacteriano / genética DNA bacteriano / isolamento e purificação Método Duplo-cego Humanos Mycobacterium tuberculosis / genética Mycobacterium tuberculosis / isolamento & purificação Estudos prospectivos Reação em Cadeia da Polimerase em Tempo Real Reprodutibilidade dos resultados Sensibilidade e Especificidade Tuberculose / diagnóstico |
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Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. |
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2018 |
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2018 |
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2019-04-22T13:30:48Z |
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2019-04-22T13:30:48Z |
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info:eu-repo/semantics/article |
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SANTOS, F. C. F. et al. Performance of the IS6110-TaqMan® Assay in the Diagnosis of Extrapulmonary Tuberculosis from Different Biological Samples. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 3, p. 331-337, jun. 2018. |
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https://www.arca.fiocruz.br/handle/icict/32656 |
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1678-9849 |
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10.1590/0037-8682-0372-2017 |
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SANTOS, F. C. F. et al. Performance of the IS6110-TaqMan® Assay in the Diagnosis of Extrapulmonary Tuberculosis from Different Biological Samples. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 3, p. 331-337, jun. 2018. 1678-9849 10.1590/0037-8682-0372-2017 |
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