Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.

Detalhes bibliográficos
Autor(a) principal: Assis, Gabriela Maíra Pereira de
Data de Publicação: 2016
Outros Autores: Alvarenga, Denise Anete Madureira de, Costa, Daniela Camargos, Souza Junior, Júlio César de, Hirano, Zelinda Maria Braga, Kano, Flora Satiko, Sousa, Taís Nóbrega de, Brito, Cristiana Ferreira Alves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20058
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil
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spelling Assis, Gabriela Maíra Pereira deAlvarenga, Denise Anete Madureira deCosta, Daniela CamargosSouza Junior, Júlio César deHirano, Zelinda Maria BragaKano, Flora SatikoSousa, Taís Nóbrega deBrito, Cristiana Ferreira Alves de2017-07-12T17:30:23Z2017-07-12T17:30:23Z2016ASSIS, Gabriela Maíra Pereira de et al. Detection of Plasmodium in faeces of the New World primate Alouatta clamitans. Mem. Inst. Oswaldo Cruz, v. 111, n. 9, p. 570-576, 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/2005810.1590/0074-02760160222engInstituto Oswaldo CruzPlasmodium vivaxNew World primatesAlouatta clamitanssimian malariaPlasmodium simiumDetection of Plasmodium in faeces of the New World primate Alouatta clamitans.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilUniversidade Regional de Blumenau. Blumenau, SC, Brasil/Centro de Pesquisas Biológicas de Indaial. Indaial, SC, BrasilUniversidade Regional de Blumenau. Blumenau, SC, Brasil/Centro de Pesquisas Biológicas de Indaial. Indaial, SC, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, BrasilPlasmodium falciparum and Plasmodium vivax have evolved with host switches between non-human primates (NHPs) and humans. Studies on the infection dynamics of Plasmodium species in NHPs will improve our understanding of the evolution of these parasites; however, such studies are hampered by the difficulty of handling animals in the field. The aim of this study was to detect genomic DNA of Plasmodium species from the faeces of New World monkeys. Faecal samples from 23 Alouatta clamitans from the Centre for Biological Research of Indaial (Santa Catarina, Brazil) were collected. Extracted DNA from faecal samples was used for molecular diagnosis of malaria by nested polymerase chain reaction. One natural infection with Plasmodium simium was identified by amplification of DNA extracted from the faeces of A. clamitans. Extracted DNA from a captive NHP was also used for parasite genotyping. The detection limit of the technique was evaluated in vitro using an artificial mixture of cultured P. falciparum in NHP faeces and determined to be 6.5 parasites/µL. Faecal samples of New World primates can be used to detect malaria infections in field surveys and also to monitor the genetic variability of parasites and dynamics of infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/20058/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALAlves_Gabriela_Detection_CPqRR_2016.pdfAlves_Gabriela_Detection_CPqRR_2016.pdfapplication/pdf6706059https://www.arca.fiocruz.br/bitstream/icict/20058/2/Alves_Gabriela_Detection_CPqRR_2016.pdf9d1144949e778e5acdf00f50af02e6a3MD52TEXTAlves_Gabriela_Detection_CPqRR_2016.pdf.txtAlves_Gabriela_Detection_CPqRR_2016.pdf.txtExtracted texttext/plain133https://www.arca.fiocruz.br/bitstream/icict/20058/3/Alves_Gabriela_Detection_CPqRR_2016.pdf.txt917e02183ff19a9448dc4c8af9e56015MD53icict/200582019-06-19 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dc.title.pt_BR.fl_str_mv Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.
title Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.
spellingShingle Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.
Assis, Gabriela Maíra Pereira de
Plasmodium vivax
New World primates
Alouatta clamitans
simian malaria
Plasmodium simium
title_short Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.
title_full Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.
title_fullStr Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.
title_full_unstemmed Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.
title_sort Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.
author Assis, Gabriela Maíra Pereira de
author_facet Assis, Gabriela Maíra Pereira de
Alvarenga, Denise Anete Madureira de
Costa, Daniela Camargos
Souza Junior, Júlio César de
Hirano, Zelinda Maria Braga
Kano, Flora Satiko
Sousa, Taís Nóbrega de
Brito, Cristiana Ferreira Alves de
author_role author
author2 Alvarenga, Denise Anete Madureira de
Costa, Daniela Camargos
Souza Junior, Júlio César de
Hirano, Zelinda Maria Braga
Kano, Flora Satiko
Sousa, Taís Nóbrega de
Brito, Cristiana Ferreira Alves de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Assis, Gabriela Maíra Pereira de
Alvarenga, Denise Anete Madureira de
Costa, Daniela Camargos
Souza Junior, Júlio César de
Hirano, Zelinda Maria Braga
Kano, Flora Satiko
Sousa, Taís Nóbrega de
Brito, Cristiana Ferreira Alves de
dc.subject.other.pt_BR.fl_str_mv Plasmodium vivax
topic Plasmodium vivax
New World primates
Alouatta clamitans
simian malaria
Plasmodium simium
dc.subject.en.pt_BR.fl_str_mv New World primates
Alouatta clamitans
simian malaria
Plasmodium simium
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-07-12T17:30:23Z
dc.date.available.fl_str_mv 2017-07-12T17:30:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ASSIS, Gabriela Maíra Pereira de et al. Detection of Plasmodium in faeces of the New World primate Alouatta clamitans. Mem. Inst. Oswaldo Cruz, v. 111, n. 9, p. 570-576, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20058
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760160222
identifier_str_mv ASSIS, Gabriela Maíra Pereira de et al. Detection of Plasmodium in faeces of the New World primate Alouatta clamitans. Mem. Inst. Oswaldo Cruz, v. 111, n. 9, p. 570-576, 2016.
0074-0276
10.1590/0074-02760160222
url https://www.arca.fiocruz.br/handle/icict/20058
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/20058/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/20058/2/Alves_Gabriela_Detection_CPqRR_2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/20058/3/Alves_Gabriela_Detection_CPqRR_2016.pdf.txt
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