Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24919 |
Resumo: | NIH grant U01AI088752 (AIK). |
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Aquino, Carolina LessaLindow, Janet CRandall, ArloWunder Junior, Elsio AugustoPablo, JozelynNakajima, RieJasinskas, AlgisCruz, Jaqueline SilvaDamião, Alcineia OliveiraNery Junior, Nívison Ruy RochaRibeiro, Guilherme de SousaCosta, FedericoHagan, José EReis, Mitermayer Galvão dosKo, Albert IcksangMedeiros, Marco AlbertoFelgner, Philip L2018-02-22T17:09:43Z2018-02-22T17:09:43Z2017AQUINO, C. L. et al. Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis. PLoS Neglected Tropical Diseases , v. 11, n. 1, p. e0005349, 2017.1935-2727https://www.arca.fiocruz.br/handle/icict/2491910.1371/journal.pntd.0005349NIH grant U01AI088752 (AIK).Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USAAntigen Discovery Inc. Irvine, CA, USAFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USAUniversity of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USAUniversity of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USAUniversity of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USAFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Institute of Collective Health. Salvador, BA, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Institute of Collective Health. Salvador, BA, BrazilYale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USAFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, USAFundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, BrasilUniversity of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, CA, USALeptospirosis is an important zoonotic disease worldwide. Humans usually present a mild non-specific febrile illness, but a proportion of them develop more severe outcomes, such as multi-organ failure, lung hemorrhage and death. Such complications are thought to depend on several factors, including the host immunity. Protective immunity is associated with humoral immune response, but little is known about the immune response mounted during naturally-acquired Leptospira infection. Here, we used protein microarray chip to profile the antibody responses of patients with severe and mild leptospirosis against the complete Leptospira interrogans serovar Copenhageni predicted ORFeome. We discovered a limited number of immunodominant antigens, with 36 antigens specific to patients, of which 11 were potential serodiagnostic antigens, identified at acute phase, and 33 were potential subunit vaccine targets, detected after recovery. Moreover, we found distinct antibody profiles in patients with different clinical outcomes: in the severe group, overall IgM responses do not change and IgG responses increase over time, while both IgM and IgG responses remain stable in the mild patient group. Analyses of individual patients' responses showed that >74% of patients in the severe group had significant IgG increases over time compared to 29% of patients in the mild group. Additionally, 90% of IgM responses did not change over time in the mild group, compared to ~51% in the severe group.engPublic Library of ScienceLeptospiroseLeptospira InterrogansProteomeAnticorpos, BacterianaAdultoAdolescenteLeptospirosisLeptospira interrogansProteomeAntibodies, BacterialAdultAdolescentDistinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24919/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAquino CL Distinct antibody responses of patients ....pdfAquino CL Distinct antibody responses of patients 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| dc.title.pt_BR.fl_str_mv |
Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis |
| title |
Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis |
| spellingShingle |
Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis Aquino, Carolina Lessa Leptospirose Leptospira Interrogans Proteome Anticorpos, Bacteriana Adulto Adolescente Leptospirosis Leptospira interrogans Proteome Antibodies, Bacterial Adult Adolescent |
| title_short |
Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis |
| title_full |
Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis |
| title_fullStr |
Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis |
| title_full_unstemmed |
Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis |
| title_sort |
Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis |
| author |
Aquino, Carolina Lessa |
| author_facet |
Aquino, Carolina Lessa Lindow, Janet C Randall, Arlo Wunder Junior, Elsio Augusto Pablo, Jozelyn Nakajima, Rie Jasinskas, Algis Cruz, Jaqueline Silva Damião, Alcineia Oliveira Nery Junior, Nívison Ruy Rocha Ribeiro, Guilherme de Sousa Costa, Federico Hagan, José E Reis, Mitermayer Galvão dos Ko, Albert Icksang Medeiros, Marco Alberto Felgner, Philip L |
| author_role |
author |
| author2 |
Lindow, Janet C Randall, Arlo Wunder Junior, Elsio Augusto Pablo, Jozelyn Nakajima, Rie Jasinskas, Algis Cruz, Jaqueline Silva Damião, Alcineia Oliveira Nery Junior, Nívison Ruy Rocha Ribeiro, Guilherme de Sousa Costa, Federico Hagan, José E Reis, Mitermayer Galvão dos Ko, Albert Icksang Medeiros, Marco Alberto Felgner, Philip L |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Aquino, Carolina Lessa Lindow, Janet C Randall, Arlo Wunder Junior, Elsio Augusto Pablo, Jozelyn Nakajima, Rie Jasinskas, Algis Cruz, Jaqueline Silva Damião, Alcineia Oliveira Nery Junior, Nívison Ruy Rocha Ribeiro, Guilherme de Sousa Costa, Federico Hagan, José E Reis, Mitermayer Galvão dos Ko, Albert Icksang Medeiros, Marco Alberto Felgner, Philip L |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospirose Leptospira Interrogans Proteome Anticorpos, Bacteriana Adulto Adolescente |
| topic |
Leptospirose Leptospira Interrogans Proteome Anticorpos, Bacteriana Adulto Adolescente Leptospirosis Leptospira interrogans Proteome Antibodies, Bacterial Adult Adolescent |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospirosis Leptospira interrogans Proteome Antibodies, Bacterial Adult Adolescent |
| description |
NIH grant U01AI088752 (AIK). |
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2017 |
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2017 |
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2018-02-22T17:09:43Z |
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2018-02-22T17:09:43Z |
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info:eu-repo/semantics/article |
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AQUINO, C. L. et al. Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis. PLoS Neglected Tropical Diseases , v. 11, n. 1, p. e0005349, 2017. |
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https://www.arca.fiocruz.br/handle/icict/24919 |
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1935-2727 |
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10.1371/journal.pntd.0005349 |
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AQUINO, C. L. et al. Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis. PLoS Neglected Tropical Diseases , v. 11, n. 1, p. e0005349, 2017. 1935-2727 10.1371/journal.pntd.0005349 |
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