IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis

Cunha, Antonio Ricardo Khouri; Bafica, Andre Luiz Barbosa; Silva, Maria da Purificação Pereira; Noronha, Almério Libório Lopes de; Kolb, Jean Pierre; Wietzerbin, Juana; Barral, Aldina Maria Prado; Barral Netto, Manoel; Weyenbergh, Johan Van

IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis

Detalhes bibliográficos
Autor(a) principal:	Cunha, Antonio Ricardo Khouri
Data de Publicação:	2009
Outros Autores:	Bafica, Andre Luiz Barbosa, Silva, Maria da Purificação Pereira, Noronha, Almério Libório Lopes de, Kolb, Jean Pierre, Wietzerbin, Juana, Barral, Aldina Maria Prado, Barral Netto, Manoel, Weyenbergh, Johan Van
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/1893
Resumo:	Laboratório Integrado de Microbiologia e Imunoregulação. Centro de Pesquisas Gonçalo Moniz - FIOCRUZ, Bahia, Brasil

Metadados do item

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spelling	Cunha, Antonio Ricardo KhouriBafica, Andre Luiz BarbosaSilva, Maria da Purificação PereiraNoronha, Almério Libório Lopes deKolb, Jean PierreWietzerbin, JuanaBarral, Aldina Maria PradoBarral Netto, ManoelWeyenbergh, Johan Van2011-04-13T00:00:14Z2011-04-13T00:00:14Z2009KHOURI, R. et al. IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis. Journal of Immunology, v. 182, n. 4, p. 2525-2531, 2009.0022-1767https://www.arca.fiocruz.br/handle/icict/189310.4049/jimmunol.0802860engIFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratório Integrado de Microbiologia e Imunoregulação. Centro de Pesquisas Gonçalo Moniz - FIOCRUZ, Bahia, BrasilLaboratório de Imunoparasitologia. Centro de Pesquisas Gonçalo Moniz - FIOCRUZ, Bahia, BrasilLaboratório Integrado de Microbiologia e Imunoregulação. Centro de Pesquisas Gonçalo Moniz - FIOCRUZ, Bahia, BrasilLaboratório Integrado de Microbiologia e Imunoregulação. Centro de Pesquisas Gonçalo Moniz - FIOCRUZ, Bahia, BrasilInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche en Santé 872, Centre de Recherche des Cordeliers, Paris, FrançaINSERM, Unité 365, Institut Curie, Paris, FrançaLaboratório de Imunoparasitologia. Centro de Pesquisas Gonçalo Moniz - FIOCRUZ, Bahia, BrasilLaboratório Integrado de Microbiologia e Imunoregulação. Centro de Pesquisas Gonçalo Moniz - FIOCRUZ, Bahia, BrasilLaboratório Integrado de Microbiologia e Imunoregulação. Centro de Pesquisas Gonçalo Moniz - FIOCRUZ, Bahia, BrasilType I IFNs (IFN-alpha/beta) have only recently gained considerable attention as immunomodulators in nonviral infectious diseases. IFN-beta has been shown to protect, in a NO-dependent manner, against murine Old World leishmaniasis caused by Leishmania major, but data in New World leishmaniasis are lacking. We found that IFN-beta dose-dependently increases parasite burden in Leishmania amazonensis- as well as Leishmania braziliensis-infected human macrophages, independent of endogenous or exogenous NO. However, IFN-beta significantly reduced superoxide release in Leishmania-infected as well as uninfected human macrophages. This decrease in superoxide production was paralleled by a significant IFN-beta-mediated increase in superoxide dismutase 1 (SOD1) protein levels. Additionally, IFN-beta inhibition of leishmanicidal activity was mimicked by SOD1 and antagonized by either pharmacological or small interfering RNA-mediated inhibition of SOD1. Finally, pronounced SOD1 expression in situ was demonstrated in biopsies from New World cutaneous leishmaniasis patients. These findings reveal a hitherto unknown IFN-beta/SOD1 axis in Leishmania infection and suggest that inhibition of SOD-associated pathways could serve as strategy in the treatment of L. amazonensis as well as L. braziliensis infection, major human pathogens.Imunoistoquímica Interferon betaLeishmaniose CutâneaMacrófagosSuperóxido DismutaseSuperóxidos/IMinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALIFN-b Impairs Superoxide-Dependent Parasite.pdfIFN-b Impairs Superoxide-Dependent Parasite.pdfapplication/pdf1016343https://www.arca.fiocruz.br/bitstream/icict/1893/1/IFN-b%20Impairs%20Superoxide-Dependent%20Parasite.pdf09496d8f56dbc991ddc5527a635213adMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/1893/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTIFN-b Impairs Superoxide-Dependent Parasite.pdf.txtIFN-b Impairs Superoxide-Dependent Parasite.pdf.txtExtracted texttext/plain44962https://www.arca.fiocruz.br/bitstream/icict/1893/5/IFN-b%20Impairs%20Superoxide-Dependent%20Parasite.pdf.txt59e2cdd6c8fff80f8b269f7d62857979MD55THUMBNAILIFN-b Impairs Superoxide-Dependent Parasite.pdf.jpgIFN-b Impairs Superoxide-Dependent Parasite.pdf.jpgGenerated Thumbnailimage/jpeg2180https://www.arca.fiocruz.br/bitstream/icict/1893/4/IFN-b%20Impairs%20Superoxide-Dependent%20Parasite.pdf.jpg074f469e31a5bfe6bcbf53f7c28aafb8MD54icict/18932023-03-15 14:33:23.908oai:www.arca.fiocruz.br:icict/1893Tk9OLUVYQ0xVU0lWRSBESVNUUklCVVRJT04gTElDRU5TRQoKQnkgc2lnbmluZyBhbmQgc3VibWl0dGluZyB0aGlzIGxpY2Vuc2UsIHlvdSAodGhlIGF1dGhvcihzKSBvciBjb3B5cmlnaHQKb3duZXIpIGdyYW50cyB0byBEU3BhY2UgVW5pdmVyc2l0eSAoRFNVKSB0aGUgbm9uLWV4Y2x1c2l2ZSByaWdodCB0byByZXByb2R1Y2UsCnRyYW5zbGF0ZSAoYXMgZGVmaW5lZCBiZWxvdyksIGFuZC9vciBkaXN0cmlidXRlIHlvdXIgc3VibWlzc2lvbiAoaW5jbHVkaW5nCnRoZSBhYnN0cmFjdCkgd29ybGR3aWRlIGluIHByaW50IGFuZCBlbGVjdHJvbmljIGZvcm1hdCBhbmQgaW4gYW55IG1lZGl1bSwKaW5jbHVkaW5nIGJ1dCBub3QgbGltaXRlZCB0byBhdWRpbyBvciB2aWRlby4KCllvdSBhZ3JlZSB0aGF0IERTVSBtYXksIHdpdGhvdXQgY2hhbmdpbmcgdGhlIGNvbnRlbnQsIHRyYW5zbGF0ZSB0aGUKc3VibWlzc2lvbiB0byBhbnkgbWVkaXVtIG9yIGZvcm1hdCBmb3IgdGhlIHB1cnBvc2Ugb2YgcHJlc2VydmF0aW9uLgoKWW91IGFsc28gYWdyZWUgdGhhdCBEU1UgbWF5IGtlZXAgbW9yZSB0aGFuIG9uZSBjb3B5IG9mIHRoaXMgc3VibWlzc2lvbiBmb3IKcHVycG9zZXMgb2Ygc2VjdXJpdHksIGJhY2stdXAgYW5kIHByZXNlcnZhdGlvbi4KCllvdSByZXByZXNlbnQgdGhhdCB0aGUgc3VibWlzc2lvbiBpcyB5b3VyIG9yaWdpbmFsIHdvcmssIGFuZCB0aGF0IHlvdSBoYXZlCnRoZSByaWdodCB0byBncmFudCB0aGUgcmlnaHRzIGNvbnRhaW5lZCBpbiB0aGlzIGxpY2Vuc2UuIFlvdSBhbHNvIHJlcHJlc2VudAp0aGF0IHlvdXIgc3VibWlzc2lvbiBkb2VzIG5vdCwgdG8gdGhlIGJlc3Qgb2YgeW91ciBrbm93bGVkZ2UsIGluZnJpbmdlIHVwb24KYW55b25lJ3MgY29weXJpZ2h0LgoKSWYgdGhlIHN1Ym1pc3Npb24gY29udGFpbnMgbWF0ZXJpYWwgZm9yIHdoaWNoIHlvdSBkbyBub3QgaG9sZCBjb3B5cmlnaHQsCnlvdSByZXByZXNlbnQgdGhhdCB5b3UgaGF2ZSBvYnRhaW5lZCB0aGUgdW5yZXN0cmljdGVkIHBlcm1pc3Npb24gb2YgdGhlCmNvcHlyaWdodCBvd25lciB0byBncmFudCBEU1UgdGhlIHJpZ2h0cyByZXF1aXJlZCBieSB0aGlzIGxpY2Vuc2UsIGFuZCB0aGF0CnN1Y2ggdGhpcmQtcGFydHkgb3duZWQgbWF0ZXJpYWwgaXMgY2xlYXJseSBpZGVudGlmaWVkIGFuZCBhY2tub3dsZWRnZWQKd2l0aGluIHRoZSB0ZXh0IG9yIGNvbnRlbnQgb2YgdGhlIHN1Ym1pc3Npb24uCgpJRiBUSEUgU1VCTUlTU0lPTiBJUyBCQVNFRCBVUE9OIFdPUksgVEhBVCBIQVMgQkVFTiBTUE9OU09SRUQgT1IgU1VQUE9SVEVECkJZIEFOIEFHRU5DWSBPUiBPUkdBTklaQVRJT04gT1RIRVIgVEhBTiBEU1UsIFlPVSBSRVBSRVNFTlQgVEhBVCBZT1UgSEFWRQpGVUxGSUxMRUQgQU5ZIFJJR0hUIE9GIFJFVklFVyBPUiBPVEhFUiBPQkxJR0FUSU9OUyBSRVFVSVJFRCBCWSBTVUNICkNPTlRSQUNUIE9SIEFHUkVFTUVOVC4KCkRTVSB3aWxsIGNsZWFybHkgaWRlbnRpZnkgeW91ciBuYW1lKHMpIGFzIHRoZSBhdXRob3Iocykgb3Igb3duZXIocykgb2YgdGhlCnN1Ym1pc3Npb24sIGFuZCB3aWxsIG5vdCBtYWtlIGFueSBhbHRlcmF0aW9uLCBvdGhlciB0aGFuIGFzIGFsbG93ZWQgYnkgdGhpcwpsaWNlbnNlLCB0byB5b3VyIHN1Ym1pc3Npb24uCg==Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis
title	IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis
spellingShingle	IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis Cunha, Antonio Ricardo Khouri Imunoistoquímica Interferon beta Leishmaniose Cutânea Macrófagos Superóxido Dismutase Superóxidos/IM
title_short	IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis
title_full	IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis
title_fullStr	IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis
title_full_unstemmed	IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis
title_sort	IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis
author	Cunha, Antonio Ricardo Khouri
author_facet	Cunha, Antonio Ricardo Khouri Bafica, Andre Luiz Barbosa Silva, Maria da Purificação Pereira Noronha, Almério Libório Lopes de Kolb, Jean Pierre Wietzerbin, Juana Barral, Aldina Maria Prado Barral Netto, Manoel Weyenbergh, Johan Van
author_role	author
author2	Bafica, Andre Luiz Barbosa Silva, Maria da Purificação Pereira Noronha, Almério Libório Lopes de Kolb, Jean Pierre Wietzerbin, Juana Barral, Aldina Maria Prado Barral Netto, Manoel Weyenbergh, Johan Van
author2_role	author author author author author author author author
dc.contributor.author.fl_str_mv	Cunha, Antonio Ricardo Khouri Bafica, Andre Luiz Barbosa Silva, Maria da Purificação Pereira Noronha, Almério Libório Lopes de Kolb, Jean Pierre Wietzerbin, Juana Barral, Aldina Maria Prado Barral Netto, Manoel Weyenbergh, Johan Van
dc.subject.decs.pt_BR.fl_str_mv	Imunoistoquímica Interferon beta Leishmaniose Cutânea Macrófagos Superóxido Dismutase Superóxidos/IM
topic	Imunoistoquímica Interferon beta Leishmaniose Cutânea Macrófagos Superóxido Dismutase Superóxidos/IM
description	Laboratório Integrado de Microbiologia e Imunoregulação. Centro de Pesquisas Gonçalo Moniz - FIOCRUZ, Bahia, Brasil
publishDate	2009
dc.date.issued.fl_str_mv	2009
dc.date.accessioned.fl_str_mv	2011-04-13T00:00:14Z
dc.date.available.fl_str_mv	2011-04-13T00:00:14Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	KHOURI, R. et al. IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis. Journal of Immunology, v. 182, n. 4, p. 2525-2531, 2009.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/1893
dc.identifier.issn.none.fl_str_mv	0022-1767
dc.identifier.doi.none.fl_str_mv	10.4049/jimmunol.0802860
identifier_str_mv	KHOURI, R. et al. IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis. Journal of Immunology, v. 182, n. 4, p. 2525-2531, 2009. 0022-1767 10.4049/jimmunol.0802860
url	https://www.arca.fiocruz.br/handle/icict/1893
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/1893/1/IFN-b%20Impairs%20Superoxide-Dependent%20Parasite.pdf https://www.arca.fiocruz.br/bitstream/icict/1893/2/license.txt https://www.arca.fiocruz.br/bitstream/icict/1893/5/IFN-b%20Impairs%20Superoxide-Dependent%20Parasite.pdf.txt https://www.arca.fiocruz.br/bitstream/icict/1893/4/IFN-b%20Impairs%20Superoxide-Dependent%20Parasite.pdf.jpg
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IFN-beta impairs superoxide-dependent parasite killing in human macrophages: evidence for a deleterious role of SOD1 in cutaneous leishmaniasis

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