Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/43711 |
Resumo: | FIOCRUZ and CNPq/PROEP (grant number 400910/2013-6). |
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Santos, Emanuelle de SouzaFrança, Luciana Souza de AragãoMeira, Cássio SantanaCerqueira, Jéssica VieiraVasconcelos, Juliana FragaNonaka, Carolina Kymie VasquesPontes-de-Carvalho, Lain CarlosSoares, Milena Botelho Pereira2020-09-30T11:45:32Z2020-09-30T11:45:32Z2020SANTOS, Emanuelle de Souza et al. Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease. Frontiers in Immunology, p. 1-10, 07 Apr. 2020.1664-3224https://www.arca.fiocruz.br/handle/icict/4371110.3389/fimmu.2020.00488FIOCRUZ and CNPq/PROEP (grant number 400910/2013-6).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Chronic Chagas disease cardiomyopathy (CCC) is the most frequent and severe form of this parasitic disease. CCC is caused by a progressive inflammation in the heart, resulting in alterations that can culminate in heart failure and death. The use of dendritic cells (DCs) appears as an option for the development of treatments due to their important role in regulating immune responses. Here, we investigated whether tolerogenic cells (tDCs) could interfere with the progression of CCC in an experimental model of Chagas disease. The tDCs were generated and characterized as CD11b+ CD11c+ cells, low expression of MHC-II, CD86, CD80, and CD40, and increased expression of PD-L. These cells produced low levels of IL-6 and IL-12p70 and higher levels of IL-10, compared to mature DCs (mDCs). Interestingly, tDCs inhibited lymphoproliferation andmarkedly increased the population of FoxP3+ Treg cells in vitro, compared to mature DCs. In a mouse model of CCC, treatment with tDCs reduced heart inflammation and fibrosis. Furthermore, tDCs treatment reduced the gene expression of pro-inflammatory cytokines (Ifng and Il12) and of genes related to cardiac remodeling (Col1a2 and Lgals3), while increasing the gene expression of IL-10. Finally, administration of tDCs, increased the percentage of Treg cells in the hearts and spleens of chagasic mice. Ours results show that tolerogenic dendritic cells have therapeutic potential on CCC, inhibiting disease progression.engFrontiers MediaCélulas de antígenoDoença de ChagasInflamaçãoFibroseTrypanosoma cruziCardiomiopatiaAntigen presenting cellsChagas diseaseInflammationFibrosisTrypanosoma cruziCardiomyopathyTolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43711/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos, Emanujela de Souza. Tolerogenic....pdfSantos, Emanujela de Souza. 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| dc.title.pt_BR.fl_str_mv |
Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease |
| title |
Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease |
| spellingShingle |
Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease Santos, Emanuelle de Souza Células de antígeno Doença de Chagas Inflamação Fibrose Trypanosoma cruzi Cardiomiopatia Antigen presenting cells Chagas disease Inflammation Fibrosis Trypanosoma cruzi Cardiomyopathy |
| title_short |
Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease |
| title_full |
Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease |
| title_fullStr |
Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease |
| title_full_unstemmed |
Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease |
| title_sort |
Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease |
| author |
Santos, Emanuelle de Souza |
| author_facet |
Santos, Emanuelle de Souza França, Luciana Souza de Aragão Meira, Cássio Santana Cerqueira, Jéssica Vieira Vasconcelos, Juliana Fraga Nonaka, Carolina Kymie Vasques Pontes-de-Carvalho, Lain Carlos Soares, Milena Botelho Pereira |
| author_role |
author |
| author2 |
França, Luciana Souza de Aragão Meira, Cássio Santana Cerqueira, Jéssica Vieira Vasconcelos, Juliana Fraga Nonaka, Carolina Kymie Vasques Pontes-de-Carvalho, Lain Carlos Soares, Milena Botelho Pereira |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Emanuelle de Souza França, Luciana Souza de Aragão Meira, Cássio Santana Cerqueira, Jéssica Vieira Vasconcelos, Juliana Fraga Nonaka, Carolina Kymie Vasques Pontes-de-Carvalho, Lain Carlos Soares, Milena Botelho Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Células de antígeno Doença de Chagas Inflamação Fibrose Trypanosoma cruzi Cardiomiopatia |
| topic |
Células de antígeno Doença de Chagas Inflamação Fibrose Trypanosoma cruzi Cardiomiopatia Antigen presenting cells Chagas disease Inflammation Fibrosis Trypanosoma cruzi Cardiomyopathy |
| dc.subject.en.pt_BR.fl_str_mv |
Antigen presenting cells Chagas disease Inflammation Fibrosis Trypanosoma cruzi Cardiomyopathy |
| description |
FIOCRUZ and CNPq/PROEP (grant number 400910/2013-6). |
| publishDate |
2020 |
| dc.date.accessioned.fl_str_mv |
2020-09-30T11:45:32Z |
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2020-09-30T11:45:32Z |
| dc.date.issued.fl_str_mv |
2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SANTOS, Emanuelle de Souza et al. Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease. Frontiers in Immunology, p. 1-10, 07 Apr. 2020. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/43711 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1664-3224 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.3389/fimmu.2020.00488 |
| identifier_str_mv |
SANTOS, Emanuelle de Souza et al. Tolerogenic Dendritic Cells Reduce Cardiac Inflammation and Fibrosis in Chronic Chagas Disease. Frontiers in Immunology, p. 1-10, 07 Apr. 2020. 1664-3224 10.3389/fimmu.2020.00488 |
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https://www.arca.fiocruz.br/handle/icict/43711 |
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eng |
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openAccess |
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Frontiers Media |
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Frontiers Media |
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