Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK

Detalhes bibliográficos
Autor(a) principal: Bottrel, R. L
Data de Publicação: 2001
Outros Autores: Dutra, Walderez Ornelas, Martins, Fabrícia Andrade, Gontijo, B, Carvalho Filho, Edgar Marcelino, Barral Netto, Manoel, Barral, Aldina Maria Prado, Almeida, Roque Pacheco de, Mayrink, Wilson, Locksley, R, Gollob, Kenneth J
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7442
Resumo: Universidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, Brasil
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spelling Bottrel, R. LDutra, Walderez OrnelasMartins, Fabrícia AndradeGontijo, BCarvalho Filho, Edgar MarcelinoBarral Netto, ManoelBarral, Aldina Maria PradoAlmeida, Roque Pacheco deMayrink, WilsonLocksley, RGollob, Kenneth J2014-03-25T12:59:44Z2014-03-25T12:59:44Z2001BOTTREL, R. L. et al. Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK. Infection and Immunity, v. 69, n. 5, p.3232-3239, 2001.0019-9567https://www.arca.fiocruz.br/handle/icict/744210.1128/IAI.69.5.3232–3239.2001engAmerican Society for MicrobiologyFlow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACKinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Department of Morphology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, BrasilUniversidade Federal da Bahia. Medical Center. Salvador, BA, BrasilUniversidade Federal da Bahia. Medical Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Medical Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Medical Center. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Department of Parasitology. Belo Horizonte, MG, BrasilUCSF Medical Center. San Francisco, CaliforniaUniversidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, BrasilLeishmaniasis, caused by infection with the protozoan parasite Leishmania, affects millions of individuals worldwide, causing serious morbidity and mortality. This study directly determined the frequency of cells producing key immunoregulatory cytokines in response to the recombinant antigen Leishmania homolog of receptors for activated kinase C (LACK) and soluble leishmania antigen (SLA), and it determined relative contributions of these antigens to the overall cytokine profile in individuals infected for the first time with Leishmania braziliensis. All individuals presented with the cutaneous clinical form of leishmaniasis and were analyzed for proliferative responses to LACK antigen and SLA, frequency of lymphocyte subpopulations (analyzed ex vivo), and antigen-induced (LACK and SLA) cytokine production at the single-cell level (determined by flow cytometry). The following were determined. (i) The Th1-type response previously seen in patients with cutaneous leishmaniasis is due to gamma interferon (IFN-g) production by several different sources, listed in order of contribution: CD41 T lymphocytes, CD42, CD82 lymphocytes, and CD81 T lymphocytes. (ii) SLA induced a higher frequency of lymphocytes producing IFN-g and tumor necrosis factor alpha (TNF-a) than did LACK. (iii) LACK induced an activation of monocyte populations as reflected by an increased percentage of CD14-positive cells. (iv) Neither SLA nor LACK induced detectable frequencies of cells producing interleukin-4 (IL-4) or IL-5. These data demonstrated a multifaceted immune response to SLA in human leishmaniasis involving Th1 CD41 T lymphocytes (IFN-g1 and IL-102/IL-42), Tc1 CD81 T cells (IFN-g1, and IL-102/IL-42), and a high frequency of TNF-a-producing lymphocytes. Moreover, it was determined that the recombinant antigen LACK acts as a weak inducer of Th1-type lymphocyte responses compared to SLA.Antígenos de Protozoários/imunologiaCitocinas/biossínteseCitometria de FluxoLeishmaniose Cutânea/imunologiaProteínas de Protozoários/imunologiaHumanosCélulas Th1/imunologiaInterferon gama/biossínteseInterleucina-10/biossínteseProteínas Recombinantes/imunologiaFator de Necrose Tumoral alfa/biossínteseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBottrel R L Flow Cytometric....pdfBottrel R L Flow Cytometric....pdfapplication/pdf154360https://www.arca.fiocruz.br/bitstream/icict/7442/1/Bottrel%20R%20L%20Flow%20Cytometric....pdfbff78f52e426df1d05e0ec03222f782aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7442/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBottrel R L Flow Cytometric....pdf.txtBottrel R L Flow Cytometric....pdf.txtExtracted texttext/plain39950https://www.arca.fiocruz.br/bitstream/icict/7442/5/Bottrel%20R%20L%20Flow%20Cytometric....pdf.txtb139c1dcd0296d69a12f7f7a1da3ba15MD55THUMBNAILBottrel R L Flow Cytometric....pdf.jpgBottrel R L Flow Cytometric....pdf.jpgGenerated Thumbnailimage/jpeg2230https://www.arca.fiocruz.br/bitstream/icict/7442/4/Bottrel%20R%20L%20Flow%20Cytometric....pdf.jpg5e482c03c52f3063899547a168a0b8e0MD54icict/74422023-03-15 14:33:56.626oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:56Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
title Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
spellingShingle Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
Bottrel, R. L
Antígenos de Protozoários/imunologia
Citocinas/biossíntese
Citometria de Fluxo
Leishmaniose Cutânea/imunologia
Proteínas de Protozoários/imunologia
Humanos
Células Th1/imunologia
Interferon gama/biossíntese
Interleucina-10/biossíntese
Proteínas Recombinantes/imunologia
Fator de Necrose Tumoral alfa/biossíntese
title_short Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
title_full Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
title_fullStr Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
title_full_unstemmed Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
title_sort Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK
author Bottrel, R. L
author_facet Bottrel, R. L
Dutra, Walderez Ornelas
Martins, Fabrícia Andrade
Gontijo, B
Carvalho Filho, Edgar Marcelino
Barral Netto, Manoel
Barral, Aldina Maria Prado
Almeida, Roque Pacheco de
Mayrink, Wilson
Locksley, R
Gollob, Kenneth J
author_role author
author2 Dutra, Walderez Ornelas
Martins, Fabrícia Andrade
Gontijo, B
Carvalho Filho, Edgar Marcelino
Barral Netto, Manoel
Barral, Aldina Maria Prado
Almeida, Roque Pacheco de
Mayrink, Wilson
Locksley, R
Gollob, Kenneth J
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bottrel, R. L
Dutra, Walderez Ornelas
Martins, Fabrícia Andrade
Gontijo, B
Carvalho Filho, Edgar Marcelino
Barral Netto, Manoel
Barral, Aldina Maria Prado
Almeida, Roque Pacheco de
Mayrink, Wilson
Locksley, R
Gollob, Kenneth J
dc.subject.decs.pt_BR.fl_str_mv Antígenos de Protozoários/imunologia
Citocinas/biossíntese
Citometria de Fluxo
Leishmaniose Cutânea/imunologia
Proteínas de Protozoários/imunologia
Humanos
Células Th1/imunologia
Interferon gama/biossíntese
Interleucina-10/biossíntese
Proteínas Recombinantes/imunologia
Fator de Necrose Tumoral alfa/biossíntese
topic Antígenos de Protozoários/imunologia
Citocinas/biossíntese
Citometria de Fluxo
Leishmaniose Cutânea/imunologia
Proteínas de Protozoários/imunologia
Humanos
Células Th1/imunologia
Interferon gama/biossíntese
Interleucina-10/biossíntese
Proteínas Recombinantes/imunologia
Fator de Necrose Tumoral alfa/biossíntese
description Universidade Federal de Minas Gerais. Department of Biochemistry-Immunology. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Medical Center. Belo Horizonte, MG, Brasil
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2014-03-25T12:59:44Z
dc.date.available.fl_str_mv 2014-03-25T12:59:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BOTTREL, R. L. et al. Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK. Infection and Immunity, v. 69, n. 5, p.3232-3239, 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7442
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.69.5.3232–3239.2001
identifier_str_mv BOTTREL, R. L. et al. Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK. Infection and Immunity, v. 69, n. 5, p.3232-3239, 2001.
0019-9567
10.1128/IAI.69.5.3232–3239.2001
url https://www.arca.fiocruz.br/handle/icict/7442
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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