Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10444 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil |
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Soares, Juliana Baptista Rocha CorrêaMonteiro, Clarissa Menezes MayaCunha, Paula do Rego Barros BittencourtAtella, Georgia CorreaLara, Flávio AlvesAvila, Joana da Costa Pinto d´Menezes, Diego SilvaSantos, Marcos André Vannier dosOliveira, Pedro Lagerblad deEgan, Timothy JOliveira, Marcus Fernandes de2015-05-20T18:26:38Z2015-05-20T18:26:38Z2007SOARES, J. B. R.C. Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni. FEBS Letters,v. 581, n. 9, p. 1742-1750, 2007.0014-5793https://www.arca.fiocruz.br/handle/icict/1044410.1016/j.febslet.2007.03.054engElsevierExtracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacteriose. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biomorfologia Parasitária. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrasilUniversity of Cape Town. Department of Chemistry. Cape Tow, SudáfricaUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrasilHemozoin (Hz) is a heme crystal produced upon hemoglobin digestion as the main mechanism of heme disposal in several hematophagous organisms. Here, we show that, in the helminth Schistosoma mansoni, Hz formation occurs in extracellular lipid droplets (LDs). Transmission electron microscopy of adult worms revealed the presence of numerous electronlucent round structures similar to LDs in gut lumen, where multicrystalline Hz assemblies were found associated to their surfaces. Female regurgitates promoted Hz formation in vitro in reactions partially inhibited by boiling. Fractionation of regurgitates showed that Hz crystallization activity was essentially concentrated on lower density fractions, which have small amounts of pre-formed Hz crystals, suggesting that hydrophilic– hydrophobic interfaces, and not Hz itself, play a key catalytic role in Hz formation in S. mansoni. Thus, these data demonstrate that LDs present in the gut lumen of S. mansoni support Hz formation possibly by allowing association of heme to the lipid–water interface of these structuresHemozoinHemeLipid dropletsSchistosoma mansoniHemoproteínasLípidosSchistosoma mansoniHemozoininfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10444/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALSoares JBC Extracellular lipid droplets.....pdfSoares JBC Extracellular lipid droplets.....pdfapplication/pdf964569https://www.arca.fiocruz.br/bitstream/icict/10444/2/Soares%20JBC%20Extracellular%20lipid%20droplets.....pdf2df34c7554a7f22d1d32e41a2f95415bMD52TEXTSoares JBC Extracellular lipid droplets.....pdf.txtSoares JBC Extracellular lipid droplets.....pdf.txtExtracted texttext/plain47863https://www.arca.fiocruz.br/bitstream/icict/10444/3/Soares%20JBC%20Extracellular%20lipid%20droplets.....pdf.txt96b6817dec4c5315cd7cb9b3ad02ed38MD53icict/104442023-03-15 14:33:25.315oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni |
| title |
Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni |
| spellingShingle |
Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni Soares, Juliana Baptista Rocha Corrêa Hemozoin Heme Lipid droplets Schistosoma mansoni Hemoproteínas Lípidos Schistosoma mansoni Hemozoin |
| title_short |
Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni |
| title_full |
Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni |
| title_fullStr |
Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni |
| title_full_unstemmed |
Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni |
| title_sort |
Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni |
| author |
Soares, Juliana Baptista Rocha Corrêa |
| author_facet |
Soares, Juliana Baptista Rocha Corrêa Monteiro, Clarissa Menezes Maya Cunha, Paula do Rego Barros Bittencourt Atella, Georgia Correa Lara, Flávio Alves Avila, Joana da Costa Pinto d´ Menezes, Diego Silva Santos, Marcos André Vannier dos Oliveira, Pedro Lagerblad de Egan, Timothy J Oliveira, Marcus Fernandes de |
| author_role |
author |
| author2 |
Monteiro, Clarissa Menezes Maya Cunha, Paula do Rego Barros Bittencourt Atella, Georgia Correa Lara, Flávio Alves Avila, Joana da Costa Pinto d´ Menezes, Diego Silva Santos, Marcos André Vannier dos Oliveira, Pedro Lagerblad de Egan, Timothy J Oliveira, Marcus Fernandes de |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Soares, Juliana Baptista Rocha Corrêa Monteiro, Clarissa Menezes Maya Cunha, Paula do Rego Barros Bittencourt Atella, Georgia Correa Lara, Flávio Alves Avila, Joana da Costa Pinto d´ Menezes, Diego Silva Santos, Marcos André Vannier dos Oliveira, Pedro Lagerblad de Egan, Timothy J Oliveira, Marcus Fernandes de |
| dc.subject.en.pt_BR.fl_str_mv |
Hemozoin Heme Lipid droplets Schistosoma mansoni |
| topic |
Hemozoin Heme Lipid droplets Schistosoma mansoni Hemoproteínas Lípidos Schistosoma mansoni Hemozoin |
| dc.subject.decs.pt_BR.fl_str_mv |
Hemoproteínas Lípidos Schistosoma mansoni Hemozoin |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil |
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2007 |
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2007 |
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2015-05-20T18:26:38Z |
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2015-05-20T18:26:38Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SOARES, J. B. R.C. Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni. FEBS Letters,v. 581, n. 9, p. 1742-1750, 2007. |
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https://www.arca.fiocruz.br/handle/icict/10444 |
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0014-5793 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.febslet.2007.03.054 |
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SOARES, J. B. R.C. Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni. FEBS Letters,v. 581, n. 9, p. 1742-1750, 2007. 0014-5793 10.1016/j.febslet.2007.03.054 |
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Elsevier |
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