Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model

Detalhes bibliográficos
Autor(a) principal: Jácome, Fernanda Cunha
Data de Publicação: 2021
Outros Autores: Caldas, Gabriela Cardoso, Rasinhas, Arthur da Costa, Almeida, Ana LuisaTeixeira de, Souza, Daniel Dias Coutinho de, Paulino, Amanda Carlos, Leonardo, Raphael, Barth, Ortrud Monika, Santos, Flavia Barreto dos, Vieira, Débora Ferreira Barreto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/47366
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.
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spelling Jácome, Fernanda CunhaCaldas, Gabriela CardosoRasinhas, Arthur da CostaAlmeida, Ana LuisaTeixeira deSouza, Daniel Dias Coutinho dePaulino, Amanda CarlosLeonardo, RaphaelBarth, Ortrud MonikaSantos, Flavia Barreto dosVieira, Débora Ferreira Barreto2021-05-25T19:07:31Z2021-05-25T19:07:31Z2021JÁCOME, Fernanda Cunha et al. Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model. Scientific Reports, v. 11, n. 9723, 18p, 2021.2045-2322https://www.arca.fiocruz.br/handle/icict/4736610.1038/s41598-021-88502-2engNatureLinhagens DENV-2FígadoModelo murino imunocompetenteAnálise comparativaComparative analysisLiver diseaseModelo murino imunocompetenteDENV‑2 lineagesComparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Dengue (DEN) is the most prevalent arbovirus among humans, and four billion people live at risk of infection. The clinical manifestations of DEN are variable, and the disease may present subclinically or asymptomatically. A quarter of patients develop classical dengue (CD) or severe dengue (SD), which is potentially lethal and involves vascular permeability changes, severe hemorrhage and organ damage. The involvement of the liver is a fairly common feature in DEN, and alterations range from asymptomatic elevation of transaminases to acute liver failure. Since its introduction in Brazil in 1990, two strains of Dengue virus (DENV) serotype 2 (DENV-2) have been detected: Lineage I, which is responsible for an outbreak in 1991, and Lineage II, which caused an epidemic greater than the previous one and had a diferent epidemiological profle. To date, studies on diferent strains of the same serotype/genotype and their association with disease severity are scarce. In addition, one of the greatest challenges regarding the study of DEN pathogenesis and the development of drug and vaccine therapies is the absence of an animal model that reproduces the disease as it occurs in humans. The main goals of this study were to assess BALB/c mouse susceptibility experimentally infected by two distinct DENV-2 strains and characterize possible diferences in the clinical signs and alterations induced in the liver resulting from those infections. Mice infected by the two DENV-2 lineages gained less weight than uninfected mice; however, their livers were slightly heavier. Increased AST and AST levels were observed in infected mice, and the number of platelets increased in the frst 72 h of infection and subsequently decreased. Mice infected with both lineages presented leukocytosis but at diferent times of infection. The histopathological changes induced by both lineages were similar and comparable to the changes observed in DEN fatal cases. The viral genome was detected in two liver samples. The results demonstrate the susceptibility of BALB/c mice to both DENV-2 lineages and suggest that the changes induced by those strains are similar, although for some parameters, they are manifested at diferent times of infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47366/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFernandaC_Jacome_etal_IOC_2021..pdfFernandaC_Jacome_etal_IOC_2021..pdfapplication/pdf2919948https://www.arca.fiocruz.br/bitstream/icict/47366/2/FernandaC_Jacome_etal_IOC_2021..pdf11e8514eb51b783276af49dc215203f9MD52TEXTFernandaC_Jacome_etal_IOC_2021..pdf.txtFernandaC_Jacome_etal_IOC_2021..pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model
title Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model
spellingShingle Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model
Jácome, Fernanda Cunha
Linhagens DENV-2
Fígado
Modelo murino imunocompetente
Análise comparativa
Comparative analysis
Liver disease
Modelo murino imunocompetente
DENV‑2 lineages
title_short Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model
title_full Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model
title_fullStr Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model
title_full_unstemmed Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model
title_sort Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model
author Jácome, Fernanda Cunha
author_facet Jácome, Fernanda Cunha
Caldas, Gabriela Cardoso
Rasinhas, Arthur da Costa
Almeida, Ana LuisaTeixeira de
Souza, Daniel Dias Coutinho de
Paulino, Amanda Carlos
Leonardo, Raphael
Barth, Ortrud Monika
Santos, Flavia Barreto dos
Vieira, Débora Ferreira Barreto
author_role author
author2 Caldas, Gabriela Cardoso
Rasinhas, Arthur da Costa
Almeida, Ana LuisaTeixeira de
Souza, Daniel Dias Coutinho de
Paulino, Amanda Carlos
Leonardo, Raphael
Barth, Ortrud Monika
Santos, Flavia Barreto dos
Vieira, Débora Ferreira Barreto
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jácome, Fernanda Cunha
Caldas, Gabriela Cardoso
Rasinhas, Arthur da Costa
Almeida, Ana LuisaTeixeira de
Souza, Daniel Dias Coutinho de
Paulino, Amanda Carlos
Leonardo, Raphael
Barth, Ortrud Monika
Santos, Flavia Barreto dos
Vieira, Débora Ferreira Barreto
dc.subject.other.pt_BR.fl_str_mv Linhagens DENV-2
Fígado
Modelo murino imunocompetente
Análise comparativa
topic Linhagens DENV-2
Fígado
Modelo murino imunocompetente
Análise comparativa
Comparative analysis
Liver disease
Modelo murino imunocompetente
DENV‑2 lineages
dc.subject.en.pt_BR.fl_str_mv Comparative analysis
Liver disease
Modelo murino imunocompetente
DENV‑2 lineages
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-05-25T19:07:31Z
dc.date.available.fl_str_mv 2021-05-25T19:07:31Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv JÁCOME, Fernanda Cunha et al. Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model. Scientific Reports, v. 11, n. 9723, 18p, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47366
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-021-88502-2
identifier_str_mv JÁCOME, Fernanda Cunha et al. Comparative analysis of liver involvement caused by two DENV‑2 lineages using an immunocompetent murine model. Scientific Reports, v. 11, n. 9723, 18p, 2021.
2045-2322
10.1038/s41598-021-88502-2
url https://www.arca.fiocruz.br/handle/icict/47366
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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