Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24803 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil. |
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Souza, Hugo Amorim dos Santos deCorrea, Edmar Henrique CostaBianco Junior, CesareAndrade, Márcia Cristina RibeiroLima Junior, Josué da CostaRiccio, Lilian Rose PrattDaniel-Ribeiro, Cláudio TadeuTotino, Paulo Renato Rivas2018-02-11T14:10:00Z2018-02-11T14:10:00Z2017SOUZA, Hugo Amorim dos Santos de; et al. Detection of signal regulatory protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human monoclonal antibody. Frontiers in Immunology, v.8, Article 1814, 7p. Dec. 2017.1664-3224https://www.arca.fiocruz.br/handle/icict/2480310.3389/fimmu.2017.01814engFrontiers Mediaprimatas não-humanosCitometria de fluxoresposta imuneproteína reguladora de sinal αnon-human primatesSaimiri sciureusimmune responsesignal regulatory protein αflow cytometryDetection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibodyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Ciência e Tecnologia em Biomodelos. Serviço de Primatologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Non-human primates (NHP) are suitable models for studying different aspects of the human system, including pathogenesis and protective immunity to many diseases. However, the lack of specific immunological reagents for neo-tropical monkeys, such as Saimiri sciureus, is still a major factor limiting studies in these models. An alternative strategy to circumvent this obstacle has been the selection of immunological reagents directed to humans, which present cross-reactivity with NHP molecules. In this context and considering the key role of inhibitory immunoreceptors-such as the signal regulatory protein α (SIRPα)-in the regulation of immune responses, in the present study, we attempted to evaluate the ability of anti-human SIRPα monoclonal antibodies to recognize SIRPα in antigen-presenting S. sciureus peripheral blood mononuclear cells (PBMC). As shown by flow cytometry analysis, the profile of anti-SIRPα staining as well as the levels of SIRPα-positive cells in PBMC from S. sciureus were similar to those observed in human PBMC. Furthermore, using anti-SIRPα monoclonal antibody, it was possible to detect a decrease of the SIRPα levels on surface of S. sciureus cells after in vitro stimulation with lipopolysaccharides. Finally, using computed-based analysis, we observed a high degree of conservation of SIRPα across six species of primates and the presence of shared epitopes in the extracellular domain between humans and Saimiri genus that could be targeted by antibodies. In conclusion, we have identified a commercially available anti-human monoclonal antibody that is able to detect SIRPα of S. sciureus monkeys and that, therefore, can facilitate the study of the immunomodulatory role of SIRPα when S. sciureus is used as a model.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24803/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALhugo_amorim_etal_IOC_2017.pdfhugo_amorim_etal_IOC_2017.pdfapplication/pdf1492323https://www.arca.fiocruz.br/bitstream/icict/24803/2/hugo_amorim_etal_IOC_2017.pdf838a70625a19b6bca9102033971bae50MD52TEXThugo_amorim_etal_IOC_2017.pdf.txthugo_amorim_etal_IOC_2017.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
| title |
Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
| spellingShingle |
Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody Souza, Hugo Amorim dos Santos de primatas não-humanos Citometria de fluxo resposta imune proteína reguladora de sinal α non-human primates Saimiri sciureus immune response signal regulatory protein α flow cytometry |
| title_short |
Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
| title_full |
Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
| title_fullStr |
Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
| title_full_unstemmed |
Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
| title_sort |
Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
| author |
Souza, Hugo Amorim dos Santos de |
| author_facet |
Souza, Hugo Amorim dos Santos de Correa, Edmar Henrique Costa Bianco Junior, Cesare Andrade, Márcia Cristina Ribeiro Lima Junior, Josué da Costa Riccio, Lilian Rose Pratt Daniel-Ribeiro, Cláudio Tadeu Totino, Paulo Renato Rivas |
| author_role |
author |
| author2 |
Correa, Edmar Henrique Costa Bianco Junior, Cesare Andrade, Márcia Cristina Ribeiro Lima Junior, Josué da Costa Riccio, Lilian Rose Pratt Daniel-Ribeiro, Cláudio Tadeu Totino, Paulo Renato Rivas |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Souza, Hugo Amorim dos Santos de Correa, Edmar Henrique Costa Bianco Junior, Cesare Andrade, Márcia Cristina Ribeiro Lima Junior, Josué da Costa Riccio, Lilian Rose Pratt Daniel-Ribeiro, Cláudio Tadeu Totino, Paulo Renato Rivas |
| dc.subject.other.pt_BR.fl_str_mv |
primatas não-humanos Citometria de fluxo resposta imune proteína reguladora de sinal α |
| topic |
primatas não-humanos Citometria de fluxo resposta imune proteína reguladora de sinal α non-human primates Saimiri sciureus immune response signal regulatory protein α flow cytometry |
| dc.subject.en.pt_BR.fl_str_mv |
non-human primates Saimiri sciureus immune response signal regulatory protein α flow cytometry |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil. |
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2017 |
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2017 |
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2018-02-11T14:10:00Z |
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2018-02-11T14:10:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SOUZA, Hugo Amorim dos Santos de; et al. Detection of signal regulatory protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human monoclonal antibody. Frontiers in Immunology, v.8, Article 1814, 7p. Dec. 2017. |
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https://www.arca.fiocruz.br/handle/icict/24803 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2017.01814 |
| identifier_str_mv |
SOUZA, Hugo Amorim dos Santos de; et al. Detection of signal regulatory protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human monoclonal antibody. Frontiers in Immunology, v.8, Article 1814, 7p. Dec. 2017. 1664-3224 10.3389/fimmu.2017.01814 |
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